#Coronavirus Disease Research #References (by AMEDEO, January 12 '25)

 

BMJ

1. KAST KA, Sidelnik SA, Nejad SH, Suzuki J, et al
   Management of alcohol withdrawal syndromes in general hospital settings.
   BMJ. 2025;388:e080461.
   PubMed         Abstract available
   
   

   
   Clin Infect Dis

2. XIANG W, Steinbeis F, Dhindsa K, Kurth F, et al
   Predicting the risk of intensive care unit admission in patients with COVID-19 presenting in the emergency room: Development and evaluation of the CROSS score.
   Clin Infect Dis. 2025 Jan 10:ciaf006. doi: 10.1093.
   PubMed         Abstract available
   
   

   
   Infect Control Hosp Epidemiol

3. NAKASHITA M, Kurosawa K, Fukusumi M, Irie F, et al
   Improving the ability of psychiatric hospitals to respond to infectious disease outbreaks: lessons learned from the COVID-19 outbreak response in Ibaraki Prefecture, Japan.
   Infect Control Hosp Epidemiol. 2025 Jan 8:1-2. doi: 10.1017/ice.2024.
   PubMed        
   
   
4. VAN REST A, Clarke A, Gounder P, Nie PK, et al
   COVID-19 outbreaks in nursing homes in Los Angeles County, March 2020-April 2022.
   Infect Control Hosp Epidemiol. 2025 Jan 7:1-7. doi: 10.1017/ice.2024.
   PubMed         Abstract available
   
   

   
   J Infect

5. ONG DS, Harris M, Hart JD, Russell FM, et al
   Lack of correlation between school reopening and trends in adult COVID-19 hospitalisations and death rates during the Delta and early Omicron periods: an ecological analysis of five countries.
   J Infect. 2025 Jan 6:106390. doi: 10.1016/j.jinf.2024.106390.
   PubMed         Abstract available
   
   
6. JORDA A, Prager M, Pracher L, Haselwanter P, et al
   Immunogenicity, Safety, and Reactogenicity of Concomitant Administration of the Novavax Vaccine against Omicron XBB.1.5 (NVX-CoV2601) and a 20-valent Pneumococcal Conjugate Vaccine in Adults Aged >/=60 Years: A Randomised, Double-blind, Placebo-contro
   J Infect. 2025 Jan 3:106405. doi: 10.1016/j.jinf.2024.106405.
   PubMed         Abstract available
   
   

   
   J Med Virol

7. ZHANG S, Kong X, Zhen Q, Wei Y, et al
   Dynamic Changes and Trends of SARS-CoV-2 Antibodies Induced by Infection and Vaccination Across Multiple Time Points.
   J Med Virol. 2025;97:e70161.
   PubMed         Abstract available
   
   
8. KO C, Cheng CC, Mistretta D, Ambike S, et al
   SARS-CoV-2 Productively Infects Human Hepatocytes and Induces Cell Death.
   J Med Virol. 2025;97:e70156.
   PubMed         Abstract available
   
   

   
   J Virol

9. GRIMES SL, Heaton BE, Anderson ML, Burke K, et al
   The coronavirus nsp14 exoribonuclease interface with the cofactor nsp10 is essential for efficient virus replication and enzymatic activity.
   J Virol. 2025 Jan 10:e0170824. doi: 10.1128/jvi.01708.
   PubMed         Abstract available
   
   
10. ARORA P, Zhang L, Nehlmeier I, Kempf A, et al
    Host cell lectins ASGR1 and DC-SIGN jointly with TMEM106B confer ACE2 independence and imdevimab resistance to SARS-CoV-2 pseudovirus with spike mutation E484D.
    J Virol. 2025 Jan 10:e0123024. doi: 10.1128/jvi.01230.
    PubMed         Abstract available
    
    
11. HU H, Leng C, Shu Y, Peng L, et al
    Structural insights into hybridoma-derived neutralizing monoclonal antibodies against Omicron BA.5 and XBB.1.16 variants of SARS-CoV-2.
    J Virol. 2025 Jan 7:e0130724. doi: 10.1128/jvi.01307.
    PubMed         Abstract available
    
    
12. GUNAWARDENE CD, Wong L-YR
    Betacoronavirus internal protein: role in immune evasion and viral pathogenesis.
    J Virol. 2025 Jan 6:e0135324. doi: 10.1128/jvi.01353.
    PubMed         Abstract available
    
    

    
    Life Sci

13. CHOPRA A, Franko N, Chow EJ
    Navigating neurologic post-COVID-19 conditions in adults: Management strategies for cognitive dysfunction, headaches and neuropathies.
    Life Sci. 2025;362:123374.
    PubMed         Abstract available
    
    

    
    Zhonghua Jie He He Hu Xi Za Zhi

14. SONG LC, Xie LX
    [Clinical update in critical care of pulmonary medicine 2024].
    Zhonghua Jie He He Hu Xi Za Zhi. 2025;48:84-89.
    PubMed         Abstract available
    
    
15. LI SN, Ni WT, Li R, Chen YW, et al
    [Clinical characteristics of immunocompromised patients infected with COVID-19].
    Zhonghua Jie He He Hu Xi Za Zhi. 2025;48:35-42.
    PubMed         Abstract available
    
    
16. 
    [Chinese expert consensus on the diagnosis and treatment of pneumonia in the elderly (2024 Edition)].
    Zhonghua Jie He He Hu Xi Za Zhi. 2025;48:18-34.
    PubMed         Abstract available

#Influenza and Other Respiratory Viruses Research #References (by AMEDEO, January 12 '25)

 

Arch Virol

1. SAKUMA S, Mine J, Uchida Y, Kumagai A, et al
   Long-term immune responses induced by low-dose infection with high pathogenicity avian influenza viruses can protect mallards from reinfection with a heterologous strain.
   Arch Virol. 2025;170:33.
   PubMed         Abstract available
   
   
2. ARVIA R, Rocca A, Casciato B, Stincarelli MA, et al
   High-resolution melting analysis for detection of nucleotide mutation markers in the polymerase-acidic (PA) gene of influenza virus that are associated with baloxavir marboxil resistance.
   Arch Virol. 2025;170:29.
   PubMed         Abstract available
   
   
3. ASHRAF MA, Raza MA, Imran A, Amjad MN, et al
   Next-generation vaccines for influenza B virus: advancements and challenges.
   Arch Virol. 2025;170:25.
   PubMed         Abstract available
   
   
4. REHMAN Z, Edington K, Jamal Z, Kritz-Wilson A, et al
   The introduction of the SARS-CoV-2 BA.4 lineage into Pakistan.
   Arch Virol. 2025;170:26.
   PubMed         Abstract available
   
   

   
   Biochemistry

5. LEE E, Rauscher S
   The Conformational Space of the SARS-CoV-2 Main Protease Active Site Loops Is Determined by Ligand Binding and Interprotomer Allostery.
   Biochemistry. 2025;64:32-46.
   PubMed         Abstract available
   
   

   
   BMJ

6. KAST KA, Sidelnik SA, Nejad SH, Suzuki J, et al
   Management of alcohol withdrawal syndromes in general hospital settings.
   BMJ. 2025;388:e080461.
   PubMed         Abstract available
   
   

   
   Epidemiol Infect

7. CHANDA MM, Shivachandra SB, Mishra A, Punnoose P, et al
   Unique duck rearing practice in irrigated rice paddy fields driving recurrent H5N1 Avian Influenza outbreaks in two districts of Kerala, India.
   Epidemiol Infect. 2025 Jan 7:1-36. doi: 10.1017/S0950268824001882.
   PubMed        
   
   
8. HUIBERTS AJ, Oosting IJ, de Melker HE, van de Wijgert JHHM, et al
   The effect of SARS-CoV-2 infection and COVID-19 vaccination during pregnancy on neonatal outcomes.
   Epidemiol Infect. 2024;153:e5.
   PubMed         Abstract available
   
   

   
   J Gen Virol

9. RANDALL RE, Young D, Pisliakova M, Andrejeva J, et al
   Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody.
   J Gen Virol. 2025;106.
   PubMed         Abstract available
   
   

   
   J Infect

10. MOK CKP, Tang YS, Tan CW, Chong KC, et al
    Comparison of safety and immunogenicity in the elderly after receiving either Comirnaty or Spikevax monovalent XBB1.5 COVID-19 vaccine.
    J Infect. 2024;90:106374.
    PubMed         Abstract available
    
    

    
    J Infect Dis

11. BILLINGS WZ, Ge Y, Knight JH, Hemme H, et al
    High dose inactivated influenza vaccine inconsistently improves heterologous antibody responses in an elderly human cohort.
    J Infect Dis. 2025 Jan 8:jiaf003. doi: 10.1093.
    PubMed         Abstract available
    
    

    
    J Virol

12. FOX CR, Yousef NN, Varudkar N, Shiffer EM, et al
    Resistance to complement-mediated lysis of parainfluenza virus 5-infected cells is acquired after transition from acute to persistent infection.
    J Virol. 2025 Jan 10:e0189524. doi: 10.1128/jvi.01895.
    PubMed         Abstract available
    
    
13. PERRY SS, Brice DC, Sakr AA, Kandeil A, et al
    Modulation of cytokeratin and cytokine/chemokine expression following influenza virus infection of differentiated human tonsillar epithelial cells.
    J Virol. 2025 Jan 10:e0146024. doi: 10.1128/jvi.01460.
    PubMed         Abstract available
    
    

    
    PLoS Comput Biol

14. ABRAHAM AA, Tan ZC, Shrestha P, Bozich ER, et al
    A multivalent binding model infers antibody Fc species from systems serology.
    PLoS Comput Biol. 2024;20:e1012663.
    PubMed         Abstract available
    
    
15. FAJARDO-FONTIVEROS O, Mattei M, Burgio G, Granell C, et al
    Machine learning mathematical models for incidence estimation during pandemics.
    PLoS Comput Biol. 2024;20:e1012687.
    PubMed         Abstract available
    
    
16. ZHANG XY, Yu LL, Wang WY, Sun GQ, et al
    Estimating the time-varying effective reproduction number via Cycle Threshold-based Transformer.
    PLoS Comput Biol. 2024;20:e1012694.
    PubMed         Abstract available
    
    
17. ZUNKER H, Schmieding R, Kerkmann D, Schengen A, et al
    Novel travel time aware metapopulation models and multi-layer waning immunity for late-phase epidemic and endemic scenarios.
    PLoS Comput Biol. 2024;20:e1012630.
    PubMed         Abstract available
    
    

    
    PLoS One

18. GOLROKHIAN-SANI AA, Morcos M, Philippi A, Al-Rawi R, et al
    Temporal trends in mental health terminology in Alzheimer's disease clinical trials.
    PLoS One. 2024;19:e0310264.
    PubMed         Abstract available
    
    
19. QUINN KL, Stukel TA, Detsky A, Chung H, et al
    Use of virtual care near the end of life before and during the COVID-19 pandemic: A population-based cohort study.
    PLoS One. 2025;20:e0313766.
    PubMed         Abstract available
    
    
20. EVEREST L, Henderson J, Ma C, Prebeg M, et al
    Relationship between mental health and substance abuse on COVID-19 vaccine hesitancy in youth: A mixed methods longitudinal cohort study.
    PLoS One. 2025;20:e0313157.
    PubMed         Abstract available
    
    
21. DZOGAN M, Lacko R, Hajduova Z
    Empirical research of foreign direct investments efficiency in the European Union on the edge of pandemic outbreak.
    PLoS One. 2025;20:e0313161.
    PubMed         Abstract available
    
    
22. TURK F
    Traumatic stress and post-traumatic growth in individuals who have had Covid-19: The mediating effect of resilience and moderating effect of psychological flexibility.
    PLoS One. 2024;19:e0310495.
    PubMed         Abstract available
    
    
23. AMEDEWONU EA, Aryeetey GC, Godi A, Sackeyfio J, et al
    Coping strategies of COVID-19 recovered patients at the Ghana Infectious Disease Centre.
    PLoS One. 2025;20:e0310921.
    PubMed         Abstract available
    
    
24. THONGDEE M, Chaiwattanarungruengpaisan S, Ketchim N, Sangkachai N, et al
    Evidence of avian and human influenza A virus infection in farmed Siamese crocodiles (Crocodylus siamensis) in Thailand.
    PLoS One. 2025;20:e0317035.
    PubMed         Abstract available
    
    
25. HERGOTT M, Andreski M, Rovers J
    Vaccine hesitancy among health paraprofessionals: A mixed methods study.
    PLoS One. 2025;20:e0312708.
    PubMed         Abstract available
    
    
26. SATO Y, Kawachi I, Saijo Y, Yoshioka E, et al
    Correlates of COVID-19 conspiracy theory beliefs in Japan: A cross-sectional study of 28,175 residents.
    PLoS One. 2024;19:e0310673.
    PubMed         Abstract available
    
    
27. PHILLIPS G, Racine E, Naughton AM, Lane J, et al
    Understanding uptake of the COVID-19 vaccination among the homeless: A mixed methods evaluation.
    PLoS One. 2025;20:e0312617.
    PubMed         Abstract available
    
    
28. KRISTENSEN K, Boodram B, Avila W, Pineros J, et al
    Perceptions of access to harm reduction services during the COVID-19 pandemic among people who inject drugs in metropolitan Chicago.
    PLoS One. 2025;20:e0293238.
    PubMed         Abstract available
    
    
29. BRATCHER A, Jones JM, Meyer WA 3rd, Waheed R, et al
    Anti-nucleocapsid SARS-CoV-2 antibody seroprevalence in previously infected persons with immunocompromising conditions-United States, 2020-2022.
    PLoS One. 2025;20:e0313620.
    PubMed         Abstract available
    
    
30. LAARMAN C, Hahne SJ, de Melker HE, Knol MJ, et al
    SARS-CoV-2 risk factors among symptomatic vaccinated adults attending community testing locations in the Netherlands from June 2021 till February 2022.
    PLoS One. 2024;19:e0311229.
    PubMed         Abstract available
    
    
31. KUMAR V, Golzarri-Arroyo L, Roth S, Imperiale TF, et al
    Effect of the COVID-19 pandemic on colorectal cancer screening in two university-affiliated health care systems.
    PLoS One. 2025;20:e0317057.
    PubMed         Abstract available
    
    
32. WAGNER K, Reinhardt Z, Negash S, Weber L, et al
    University students' health-related quality of life and its determinants. Results from a cross-sectional survey during the COVID-19 pandemic.
    PLoS One. 2025;20:e0310378.
    PubMed         Abstract available
    
    
33. MOHAMMAD KM, Akhi AA, Kamrujjaman M
    Bifurcation analysis of an influenza A (H1N1) model with treatment and vaccination.
    PLoS One. 2025;20:e0315280.
    PubMed         Abstract available
    
    
34. PARRY-NWEYE E, Liu Z, Dhaouadi Y, Guo X, et al
    Persistence of Phi6, a SARS-CoV-2 surrogate, in simulated indoor environments: Effects of humidity and material properties.
    PLoS One. 2025;20:e0313604.
    PubMed         Abstract available
    
    
35. PENG A
    Sustainable regional development from the perspective of economic resilience: Based on the impact of COVID-19.
    PLoS One. 2025;20:e0314663.
    PubMed         Abstract available
    
    
36. KAWAHARA M, Tanaka A
    Impact of partial occlusion of the face on multisensory emotion perception: Comparison of pre- and post-COVID-19 pandemic.
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37. WHITESIDE C, Klabbers G
    Exploring the perceptions of the effect of the COVID-19 pandemic on the mental well-being and medical education of medical students in Northern Ireland, in addition to the perceived barriers to seeking support; a qualitative study.
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38. BAKER JM, Nakayama JY, O'Hegarty M, McGowan A, et al
    Household transmission of SARS-CoV-2 in five US jurisdictions: Comparison of Delta and Omicron variants.
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39. O'NEILL L, Chumbler NR
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Development of avian #influenza A(#H5) virus #datasets for #Nextclade enables rapid and accurate #clade assignment

Abstract

The ongoing panzootic of highly pathogenic avian influenza (HPAI) A(H5) viruses is the largest in history, with unprecedented transmission to multiple mammalian species. Avian influenza A viruses of the H5 subtype circulate globally among birds and are classified into distinct clades based on their hemagglutinin (HA) genetic sequences. Thus, the ability to accurately and rapidly assign clades to newly sequenced isolates is key to surveillance and outbreak response. Co-circulation of endemic, low pathogenic avian influenza (LPAI) A(H5) lineages in North American and European wild birds necessitates the ability to rapidly and accurately distinguish between infections arising from these lineages and epizootic HPAI A(H5) viruses. However, currently available clade assignment tools are limited and often require command line expertise, hindering their utility for public health surveillance labs. To address this gap, we have developed datasets to enable A(H5) clade assignments with Nextclade, a drag-and-drop tool originally developed for SARS-CoV-2 genetic clade classification. Using annotated reference datasets for all historical A(H5) clades, clade 2.3.2.1 descendants, and clade 2.3.4.4 descendants provided by the Food and Agriculture Organization/World Health Organization/World Organisation for Animal Health (FAO/WHO/WOAH) H5 Working Group, we identified clade-defining mutations for every established clade to enable tree-based clade assignment. We then created three Nextclade datasets which can be used to assign clades to A(H5) HA sequences and call mutations relative to reference strains through a drag-and-drop interface. Nextclade assignments were benchmarked with 19,834 unique sequences not in the reference set using a pre-released version of LABEL, a well-validated and widely used command line software. Prospective assignment of new sequences with Nextclade and LABEL produced very well-matched assignments (match rates of 97.8% and 99.1% for the 2.3.2.1 and 2.3.4.4 datasets, respectively). The all-clades dataset also performed well (94.8% match rate) and correctly distinguished between all HPAI and LPAI strains. This tool additionally allows for the identification of polybasic cleavage site sequences and potential N-linked glycosylation sites. These datasets therefore provide an alternative, rapid method to accurately assign clades to new A(H5) HA sequences, with the benefit of an easy-to-use browser interface.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.07.631789v1

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Comprehensive #Infectome #Analysis Reveals Diverse Infectious Agents with #Zoonotic #Potential in #Wildlife

Abstract

Understanding wildlife-pathogen interactions is crucial for mitigating zoonotic risk. Through meta-transcriptomic sequencing we profiled the infectomes of 1,922 samples from 67 mammalian species across China, uncovering a remarkable diversity of viral, bacterial, fungal, and parasitic pathogens. Of the 195 pathogens identified, 62 were novel, including a bi- segmented coronavirus in diseased lesser pandas, which we propose represents a new genus – Zetacoronavirus. The orders Carnivora and Rodentia exhibited the highest pathogen diversity and were implicated in numerous host-jumping events. Comparative analysis of diseased versus healthy animals revealed a trend of higher pathogen loads in the former, with possible differences in tissue tropisms. In total, 48 zoonotic and 17 epizootic pathogens were identified, with frequent cross-species transmission, emphasizing the potential for emerging public health threats. This study highlights the urgent need for wildlife pathogen surveillance to inform proactive disease management strategies.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.09.632079v1

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The Channel of Gravelines, Petit Fort - Philippe Georges Seurat (1890)

 Source: WikiArt, https://www.wikiart.org/en/georges-seurat/the-channel-of-gravelines-petit-fort-philippe-1890

Public Domain.

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Respiratory #Shedding of Infectious #SARS-CoV-2 #Omicron #XBB.1.41.1 Lineage among Captive White-Tailed #Deer, #Texas, #USA

Abstract

White-tailed deer (Odocoileus virginianus) have high value for research, conservation, agriculture, and recreation and might be key SARS-CoV-2 reservoirs. In November 2023, we sampled 15 female deer in a captive facility in Texas, USA. All deer had neutralizing antibodies to SARS-CoV-2; respiratory swab samples from 11 deer were SARS-CoV-2–positive by quantitative reverse transcription PCR, and 1 deer also had a positive rectal swab sample. Six of the 11 respiratory swab samples yielded infectious virus; replication kinetics of most samples displayed lower growth 24–48 hours postinfection in vitro than Omicron lineages isolated from humans in Texas in the same period. Virus growth was similar between groups by 72 hours, suggesting no strong attenuation of deer-derived virus. All deer viruses clustered in XBB Omicron clade and demonstrated more mutations than expected compared with contemporaneous viruses in humans, suggesting that crossing the species barrier was accompanied by a high substitution rate.

Source: Emerging Infectious Diseases Journal, https://wwwnc.cdc.gov/eid/article/31/2/24-1458_article

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An intranasal, NLC-delivered self-amplifying #RNA #vaccine establishes protective #immunity against pre-pandemic #H5N1 and #H7N9 #influenza

Abstract

Seasonal and pandemic influenzas are continuous threats to human health, requiring rapid development of vaccines to multiple evolving viral strains. New RNA vaccine technologies have the adaptability and manufacturability to facilitate pandemic preparedness but have limited flexibility in their route of administration, reducing the ability to establish local protective immune responses such as respiratory mucosal immunity. Here, we describe monovalent and bivalent self-amplifying RNA (saRNA) vaccines against A/Vietnam/1203/2004 H5N1 and A/Anhui/2013 H7N9. These saRNA vaccines express either H5 or H7 hemagglutinin and are formulated with a nanostructured lipid carrier (NLC) that permits both intramuscular (IM) and intranasal (IN) dosing. In mice, IM vaccination established systemic humoral and cellular responses but no detectable mucosal response, while IN administration induced robust systemic and mucosal immunity. The saRNA-NLC vaccines provided complete protection against morbidity and mortality in ferret challenge models, establishing this intranasally-administered saRNA-NLC vaccine platform as a potential pandemic response tool.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.07.631792v1?rss=1

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The Q226L #mutation can convert a highly pathogenic #H5 2.3.4.4e virus to #bind #human-type #receptors

Abstract

H5Nx viruses continue to wreak havoc in avian and mammalian species worldwide. The virus distinguishes itself by the ability to replicate to high titers and transmit efficiently in a wide variety of hosts in diverse climatic environments. Fortunately, transmission to and between humans is scarce. Yet, if such an event were to occur, it could spark a pandemic as humans are immunologically naive to H5 viruses. A significant determinant of transmission to and between humans is the ability of the influenza A virus hemagglutinin (HA) protein to shift from an avian-type to a human-type receptor specificity. Here, we demonstrate that a 2016 2.3.4.4e virus HA can convert to human-type receptor binding via a single Q226L mutation, in contrast to a cleavage-modified 2016 2.3.4.4b virus HA. Using glycan arrays, x-ray structural analyses, tissue- and direct glycan binding, we show that L133adelta and 227Q are vital for this phenotype. Thus, whereas the 2.3.4.4e virus HA only needs a single amino acid mutation, the modified 2.3.4.4b HA was not easily converted to human-type receptor specificity.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.10.632119v1?rss=1

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#USA, #Michigan: {Oakland County} Health Division: 11 people under health #surveillance after contact with #H5N1 #birdflu infected #poultry

Waterford Township, Mich. – The Oakland County Health Division is monitoring a situation involving highly pathogenic avian influenza (HPAI), commonly known as bird flu, identified in animals at the farm at Hess-Hathaway Park in Waterford Township. 

|--     Eleven individuals who had direct contact with the animals are being         monitored    for 10 days, beginning yesterday. --|

Initially, it was reported that two of the 11 are experiencing flu-like symptoms. Since then, the Health Division has learned that only one individual has symptoms. That person’s influenza test has been collected and results from the state lab are pending.

“The risk of contracting bird flu is very low for the general public, but it’s important to be aware of the disease in the community,” said Oakland County Director of Health and Human Services Leigh-Anne Stafford. “Protect yourself and prevent bird flu by avoiding direct contact with sick or dead birds and wash your hands thoroughly if you come into contact with them.

In response to the discovery of HPAI at the farm at Hess-Hathaway Park, Waterford Township has taken proactive measures. Supervisor Anthony Bartolotta emphasized the township’s commitment to safety.

“Until further notice, portions of the farm will be closed to protect our animals, staff and visitors. However, the rest of Hess-Hathaway Park remains open for community enjoyment,” Bartolotta said. “We appreciate the community’s cooperation, patience and understanding as we work to return our farm to regular operations. We look forward to reopening in the Spring of 2025.”

Residents are encouraged to follow these prevention tips:

-- Avoid direct contact with sick or dead birds and animals.

-- Use recommended personal protective equipment (PPE) if contact is necessary.

-- Refrain from touching surfaces contaminated by bird droppings or bodily fluids.

-- Avoid consuming raw milk or raw milk products.

-- If bird flu is suspected in a domestic flock, contact Michigan Department of Agriculture and Rural Development (MDARD) immediately at 800-292-3939 (daytime) or 517-373-0440 (after hours). Additionally, report cases of unusual or unexplained deaths among wild bird populations by contacting the Michigan Department of Natural Resources at 517-336-5030.

MDARD is closely monitoring and responding to reports of sick domestic birds and HPAI throughout the state.

Contact your health care provider if you’ve had close contact with domestic fowl or wild bird and have bird flu symptoms. 

Bird flu symptoms range from no symptoms to severe disease. Signs and symptoms of bird flu in people may include:

-- Eye redness and irritation (conjunctivitis)

-- Mild fever (100 degrees Fahrenheit or greater) or feeling feverish (fever may not always be present)

-- Cough

-- Sore throat

-- Runny or stuffy nose

-- Muscle or body aches

-- Headaches

-- Fatigue

-- Shortness of breath or difficulty breathing

-- Less common symptoms include diarrhea, nausea or vomiting.

HPAI virus is widespread in wild birds worldwide and detected in domestic poultry and other animals. It can spread in various ways from flock to flock, including by wild birds, through contact with infected animals, by equipment, and on the clothing and shoes of caretakers. 

More information about bird flu can be found on the Health Division’s website at oakgov.com/health or by contacting Nurse on Call at 800-848-5533 or noc@oakgov.com. Nurse on Call is available 8:30 a.m. to 5:00 p.m., Monday through Friday. For up-to-date public health information, follow @publichealthOC on Facebook and X.  

Read the initial alert from MDARD here. For additional bird flu information from the state, click on michigan.gov/birdflu.  

Source: Oakland County Department of Health, https://www.oakgov.com/Home/Components/News/News/1751/591

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National #ONEHEALTH #Framework to Address #Zoonotic #Diseases and Advance Public Health #Preparedness in the #USA

Executive Summary 

The first ever National One Health Framework to Address Zoonotic Diseases and Advance Public Health Preparedness in the United States (NOHF-Zoonoses), 2025-2029, establishes a structure to facilitate multisectoral and transdisciplinary coordination, collaboration, and communication across the federal government. 

Using the One Health approach, the framework addresses zoonotic diseases and other priority One Health issues in the United States (U.S.). 

The One Health approach recognizes the interdependence of the health of humans, domestic and wild animals, plants, and the wider environment (including ecosystems). 

This approach mobilizes multiple diverse sectors, disciplines, and communities to work together to promote well-being and address health and ecosystem threats. 

Previous multisectoral work in the U.S. identified the critical need to formalize federal One Health coordination to address zoonotic diseases and other One Health related issues across the U.S. Government. 

Therefore, in the 2023 Consolidated Appropriations Act and the 2021 House Appropriations Committee Report, Congress directed the Centers for Disease Control and Prevention (CDC) in coordination with other federal agencies, to develop a framework based on the One Health approach to address zoonotic diseases and advance public health preparedness. 

The Act also directed CDC to coordinate with the U.S. Department of Agriculture (USDA) and the Department of the Interior (DOI) to develop a mechanism to support coordination at the federal level related to prevention, detection, control, and response for zoonotic diseases and related One Health activities. 

The One Health approach applies to zoonotic diseases as well as many other health threats at the interconnection between people, animals, plants, and the environment.  

While the primary focus of the NOHF-Zoonoses is addressing zoonotic diseases and advancing public health preparedness, the U.S. OHCU has also incorporated other components of One Health into this framework. 

To meet these directives CDC partnered with DOI, USDA and other federal agencies beginning in November 2021, to plan the establishment of the United States One Health Coordination Unit (U.S. OHCU) and to draft the NOHFZoonoses. 

The U.S. OHCU was launched in January 2024, with joint leadership from CDC (Chair 2024-2026), DOI, and USDA.  

The U.S. OHCU is coordinated by a Chair that will rotate between CDC, USDA, and DOI on a biennial calendar year basis. 

U.S. OHCU membership includes 24 agencies related to health, agriculture, interior, wildlife, environment, development, state, commerce, defense, security, and other fields. 

The draft NOHF-Zoonoses was published in September 2023 via the Federal Register Notice process, to engage and obtain input from state, Tribe, local, and territorial (STLT), non-governmental partners, and the public.  

The NOHF-Zoonoses presents goals and objectives for application of the One Health approach to protect people, animals, and our shared environment in the U.S. from zoonotic diseases and advance public health preparedness to optimize health, food safety and security, and sustainability while also promoting biodiversity and conservation outcomes. 

The NOHF-Zoonoses was designed to align with and complement existing U.S. initiatives that incorporate the One Health approach. 

The seven goals outlined in the NOHF-Zoonoses include the following areas: 

-- (1) Coordination, Collaboration and Communication; 

-- (2) Prevention; 

-- (3) Preparedness; 

-- (4) Coordinated Outbreak Investigation, Response, and Recovery; 

-- (5) Surveillance; 

-- (6) Laboratory; and 

-- (7) Workforce. 

While this framework focuses on One Health coordination at the federal level, its success depends on robust partnerships with STLT, non-governmental organizations, academia, and private sector partners as well as collaboration with relevant international partners. 

Knowledge and best practices from the implementation of the NOHF-Zoonoses will inform future One Health priorities and strengthen the nation’s ability to address One Health threats and promote health, safety, security, and resilience at the human-animal-plant-environment interface. 

Advancing One Health collaboration in the U.S. through the U.S. OHCU and the NOHF-Zoonoses will enhance our ability to jointly prevent, detect, and respond to zoonotic disease threats and related One Health issues. This initiative will improve efficiency across the U.S. government by enhancing collaboration across all relevant sectors with governmental and non-governmental partners while optimizing resource use in order to protect the health, safety, and security of people, animals, plants, and our shared environment.

(...)

Source: US Centers for Disease Control and Prevention, https://www.cdc.gov/one-health/media/pdfs/2025/01/354391-A-NOHF-ZOONOSES-508_FINAL.pdf

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Modulation of #cytokeratin and #cytokine/chemokine expression following #influenza virus infection of differentiated #human #tonsillar epithelial cells

ABSTRACT

The tonsils have been identified as a site of replication for Epstein–Barr virus, adenovirus, human papillomavirus, and other respiratory viruses. Human tonsil epithelial cells (HTECs) are a heterogeneous group of actively differentiating cells. Here, we investigated the cellular features and susceptibility of differentiated HTECs to specific influenza viruses, including expression of avian-type and mammalian-type sialic acid (SA) receptors, viral replication dynamics, and the associated cytokine secretion profiles. We found that differentiated HTECs possess more abundant α2,3-linked SA (preferentially bound by avian influenza viruses) than α2,6-linked SA (preferentially bound by mammalian strains). This dual receptor expression suggests a role in influenza virus adaptation and tropism within the tonsils by facilitating the binding and entry of multiple influenza virus strains. Our results indicated the susceptibility of differentiated HTECs to a wide range of influenza viruses from human, swine, and avian hosts. Virus production for most strains was detected as early as 1 day post-infection (dpi), and typically peaked by 3 dpi. However, pandemic H1N1 virus showed remarkably delayed replication kinetics that did not peak until at least 7 dpi. Notably, influenza virus infection impacted the expression of cytokeratins in HTEC cultures, which correlated with altered cytokine secretion patterns. These patterns varied within the strains but were most distinct in swine H3N2 infection. In conclusion, differentiated HTECs exhibited a strain-specific pattern of influenza virus replication and innate immune responses that included changes in cytokeratin and cytokine expression. These studies shed light on the complex interplay between influenza viruses and host cells in the tonsils.

Source: Journal of Virology, https://journals.asm.org/doi/full/10.1128/jvi.01460-24?af=R

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#Cambodia records 1st #human #death from #H5N1 in 2025

PHNOM PENH, Jan. 10 (Xinhua) -- A 28-year-old man from southeast Cambodia's Kampong Cham province died of H5N1 human avian influenza on Friday, becoming the first death in 2025, the Ministry of Health said in a press statement.

"Laboratory results from the National Institute of Public Health showed on Jan. 9, 2025 that the man was positive for H5N1 virus," the statement said.

"Although our team of doctors had provided him intensive care, the patient died on Jan. 10, 2025 due to his severe condition, with the symptoms of fever, cough, and dyspnea," it added.

The patient lived in village 22 in Chamkar Leu district's Chamkar Andoung commune.

"According to queries, the patient raised and fed chicken, and he cooked sick chicken for food," the statement said.

Health authorities are looking into the source of the infection and are examining any suspected cases or people who have been in contact with the victim in order to prevent an outbreak in the community, it added.

H5N1 influenza is a flu that normally spreads between sick poultry, but it can sometimes spread from poultry to humans, and its symptoms include fever, cough, runny nose, and severe respiratory illness.

The Ministry of Health called on people to be extra vigilant and not to eat ill or dead poultry, saying that bird flu still poses a threat to people's health.

From 2003 to date, there were 73 cases of human infection with H5N1 influenza, including 44 deaths in the Southeast Asian country, according to the ministry.

Source: Xinhua, https://english.news.cn/asiapacific/20250110/b1035982821244e2828ae53394c1129f/c.html

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#Belgium - #Influenza A #H5N1 viruses of high pathogenicity (Inf. with) (non-poultry including wild birds) (2017-) - Immediate notification

 A wild sanderling in Oostende.

Source: WOAH, https://wahis.woah.org/#/in-review/6175

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#Human #Health #Surveillance During Animal #Disease #Emergencies: #Minnesota Department of Health Response to Highly Pathogenic Avian #Influenza Outbreaks, 2015 and 2022–2023

ABSTRACT

Objectives

Highly pathogenic avian influenza (HPAI) poses an occupational risk for poultry workers, responders, and others in contact with infected birds. The objective of this analysis was to describe HPAI surveillance methods and outcomes, and highlight the challenges, successes, and lessons learned during the Minnesota Department of Health’s (MDH’s) public health response to HPAI outbreaks in Minnesota poultry flocks in the years 2015 and 2022–2023.

Methods

During both outbreaks, MDH staff attempted to contact all potentially exposed people and conduct a standardized interview. People were considered exposed and at risk if they had entered a barn with poultry on any HPAI test-positive premises. With their consent, exposed persons were entered into illness monitoring until 10 days from their last exposure. In 2015, MDH monitored the health of poultry workers only. In the 2022–2023 response, MDH monitored the health of poultry workers, backyard flock owners, responders, and private contract workers. In 2022–2023, interview responses were entered into a REDCap (Research Electronic Data Capture) database in real time, which automatically entered the person into monitoring if they consented. Through REDCap, they received an automated email with a unique link to a short survey asking about any symptom development. Where appropriate, interview responses from poultry workers collected in 2015 were compared to interview responses from poultry workers collected in 2022–2023.

Results

From March 3 to June 5, 2015, MDH epidemiologists interviewed and evaluated 375 (86%) of 435 poultry workers from 110 HPAI-infected flocks. From March 25, 2022 through December 31, 2023, MDH epidemiologists interviewed and evaluated 649 (65%) of 992 poultry workers, responders, contractors, and backyard flock owners associated with 151 HPAI-infected flocks. Among poultry workers, self-reported personal protective equipment (PPE) usage declined significantly from 2015 to 2022–2023 (full PPE usage 51.8% vs. 23.9%, p < .01).

Conclusion

MDH’s long standing relationships with animal health officials and the poultry industry resulted in strong poultry worker participation rates in surveillance efforts during HPAI outbreaks in 2015 and 2022–2023. Self-reported PPE usage was low, particularly in 2022–2023. Improvements in PPE accessibility and technology are needed to protect workers and responders in the on-going HPAI outbreak.

Source: Journal of Agromedicine, https://www.tandfonline.com/doi/full/10.1080/1059924X.2024.2442406

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Testing of #Retail #Cheese, #Butter, Ice Cream, and Other Dairy #Products for Highly Pathogenic Avian #Influenza in the #US

Abstract

The recent outbreak of highly pathogenic avian influenza (HPAI) in dairy cows has created public health concerns about the potential of consumers being exposed to live virus from commercial dairy products. Previous studies support that pasteurization effectively inactivates avian influenza in milk and an earlier retail milk survey showed viral RNA, but no live virus could be detected in the dairy products tested. Because of the variety of products and processing methods in which milk is used, additional product testing was conducted to determine if HPAI viral RNA could be detected in retail dairy samples, and for positive samples by quantitative real-time RT-PCR (qRT-PCR) further testing for the presence of live virus. Revised protocols were developed to extract RNA from solid dairy products including cheese and butter. The solid dairy product was mechanically liquified with garnet and zirconium beads in a bead beater diluted 1–4 with BHI media. This preprocessing step was suitable in allowing efficient RNA extraction with standard methods. Trial studies were conducted with different cheese types with spiked-in avian influenza virus to show that inoculation of the liquified cheese into embryonating chicken eggs was not toxic to the embryos and allowed virus replication. A total of 167 retail dairy samples, including a variety of cheeses, butter, ice cream, and fluid milk were collected as part of a nationwide survey. A total of 17.4% (29/167) of the samples had detectable viral RNA by qRT-PCR targeting the matrix gene, but all PCR-positive samples were negative for live virus after testing with embryonating egg inoculation. The viral RNA was also evaluated by sequencing part of the hemagglutinin gene using a revised protocol optimized to deal with the fragmented viral RNA. The sequence analysis showed all viral RNA-positive samples were highly similar to previously reported HPAI dairy cow isolates. Using the revised protocols, it was determined that HPAI viral RNA could be detected in a variety of dairy products, but existing pasteurization methods effectively inactivate the virus assuring consumer safety.

Source: Journal of Food Protection, https://www.sciencedirect.com/science/article/pii/S0362028X24002151?via%3Dihub

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Detection of #antibodies against #H5 subtype highly pathogenic avian #influenza viruses in multiple #raccoons in Tokachi District, #Hokkaido, #Japan, from 2022 to 2023

Abstract

In recent years, infection cases of H5 subtype highly pathogenic avian influenza viruses (HPAIVs) in wild mammals have increased globally. To obtain recent epidemiological information regarding influenza A virus (IAV) infection in raccoons (Procyon lotor), the prevalence of anti-IAV antibodies in sera was analyzed among raccoons captured in Tokachi District, Hokkaido, Japan, from 2019 to 2023. Screening of serum samples using enzyme-linked immunosorbent assay and agar gel precipitation test detected anti-IAV antibodies in 5 of 114 (4.4 %) raccoons. All positive sera were from raccoons captured from 2022 to 2023. The hemagglutination inhibition test revealed that all five serum samples contained anti-H5 subtype HPAIV antibodies, and one also contained anti-H1 subtype antibodies. The neuraminidase inhibition test revealed that all five sera contained anti-N1 subtype antibodies, and one also contained anti-N8 subtype antibodies. In the virus neutralization test, these five sera showed stronger neutralization activity against the H5 subtype clade 2.3.4.4b HPAIV strain recently circulating worldwide compared to the old H5 HPAIV strain isolated in Japan in 2007. These findings suggested that raccoons could be involved in the circulation of H5 HPAIVs in nature.

Source: Virus Research, https://www.sciencedirect.com/science/article/pii/S0168170224002089?via%3Dihub

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Susceptibility of #bovine respiratory and mammary epithelial #cells to avian and #mammalian derived clade 2.3.4.4b #H5N1 highly pathogenic avian #influenza viruses

Abstract

Zoonotic transmission of avian influenza viruses into mammals is relatively rare due to anatomical differences in the respiratory tract between species. Recently, clade 2.3.4.4b highly pathogenic H5N1 avian influenza viruses were detected circulating in North American cattle. Sporadic transmission between cattle, humans, and other animals proximal to cattle or after consuming products from infected cattle has occurred, but thus far there is no evidence of human-to-human transmission. However, the virus has the potential to adapt to the mammalian respiratory tract with every transmission event that occurs, making it crucial to understand cellular and species tropism of the H5N1 2.3.4.4b viruses. We compared viral kinetics of clade 2.3.4.4b viruses isolated from birds and mammals in respiratory epithelial cells derived from cattle, human, swine, and ferret. We found that avian derived viruses could replicate in swine cells only, yet mammalian derived strains could replicate efficiently in all tracheal and nasal epithelial cells tested. Interestingly, only bovine mammary epithelial cells (MEC) and swine respiratory epithelial cells were permissive to both avian and mammalian derived strains, possibly due to increased sialic acid expression on bovine MEC compared to bovine tracheal epithelial cells (TEC). However, sialic acid expression differed between dairy and beef cows: TEC derived from a dairy cow had increased expression of alpha2,6;2,3 sialic acid receptors compared to TEC from a beef-dairy cow cross. This study highlights the ability of clade 2.3.4.4b H5N1 viruses derived from mammals but not wild birds to infect the respiratory epithelium of other mammalian hosts.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.09.632235v1?rss=1

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Asymptomatic #infection and #antibody #prevalence to co-occurring avian #influenza viruses vary substantially between sympatric #seabird species following #H5N1 #outbreaks

Abstract

Emerging infectious diseases are of major concern to animal and human health. Recent emergence of high pathogenicity avian influenza virus (HPAIV) (H5N1 clade 2.3.4.4b) led to substantial global mortality across a range of host species. Co-occurring species showed marked differences in mortality, generating an urgent need for better epidemiological understanding within affected populations. We therefore tested for antibodies, indicative of previous exposure and recovery, and for active viral infection in apparently healthy individuals (n = 350) across five co-occurring seabird species on the Isle of May, Scotland, during 2023, following H5N1 HPAIV associated mortality in the preceding summer. Antibody prevalence to AIV subtypes varied substantially between species, ranging from 1.1% in European shags (Gulosus aristotelis) (to H5) to 78.7% in black-legged kittiwakes (Rissa tridactyla) (to H16 or both H13 and H16), and between 31 and 41% for three auk species (H5, H16 or both). At least 20.4% of auks had antibodies to an as yet unidentified subtype, suggesting further subtypes circulating in the population. We found low levels of active, but asymptomatic, AIV infection in individuals (1.6–4.5%), but excluded this as H5N1. Our results emphasise the importance of testing healthy individuals to understand the prevalence of co-circulating AIV subtypes in wild populations, and the potential for future reassortment events which could alter virus behaviour and impact.

Source: Scientific Reports, https://www.nature.com/articles/s41598-025-85152-6

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#Iceland, Avian #influenza #H5N5 in #cats

 {Original text in Icelandic, translated, edited.}

The Icelandic University of Iceland's Pathology Laboratory at Keldur notified the Icelandic Food and Veterinary Authority on January 6 that a kitten that arrived at the laboratory for autopsy had been diagnosed with a severe strain of avian influenza (H5N5). 

This is the same strain that has been detected in wild birds in Iceland since September last year and on one poultry farm in early December. 

The Icelandic Food and Veterinary Authority immediately issued instructions for disease control to prevent the spread of the infection and is now working to trace the infection. 

Symptoms of the disease in this cat included loss of appetite, weakness, stiffness, tremors, seizures and other neurological symptoms. 

Cat owners are asked to contact a veterinarian immediately if they notice such symptoms in their cats.

The cat diagnosed with bird flu was a 10-week-old kitten that died on December 22. The littermate the kitten was from and another kitten from the same litter died after a short illness two days earlier. They were not tested. 

The kitten's other littermates had left the home before the illness occurred and are all asymptomatic today. 

The cats are from Ísafjörður, but the kitten diagnosed with the infection had arrived in Reykjavík. The owners of all the cats have been contacted.

The Icelandic Food and Veterinary Authority believes that it is most likely that the cats were infected by an infected wild bird. 

At present, there is no evidence of infection in more cats, but the Icelandic Food and Veterinary Authority asks cat owners and veterinarians to be on the lookout for symptoms that may indicate avian influenza infection. 

There have been a number of diagnoses in wild birds in recent months, and therefore there is some risk that cats can become infected while hunting or from carcasses they come across. 

However, the Icelandic Food and Veterinary Authority does not believe the risk is so great that there is reason to warn against letting cats outside. 

People are, however, reminded to always maintain general hygiene when interacting with animals and caring for them. 

General information about avian influenza and guidelines for disease prevention can be found on the website of the Directorate of Health . 

It is worth noting that the risk of infection for people caused by the avian influenza virus is low, according to information on the website of the European Centre for Disease Prevention and Control (ECDC) .

In recent years, avian influenza has been increasingly detected in various species of mammals around the world. The most common type is the highly pathogenic H5N1 strain. This trend clearly demonstrates the virus's ability to adapt to new animal species. One of the greatest concerns worldwide is the current outbreak of avian influenza in dairy cows in the United States caused by the highly pathogenic H5N1 strain. However, the genotype of the virus in question has not yet been identified anywhere else in the world. Detailed information about this can be found on the websites of the United States Department of Agriculture USDA and the United States Centers for Disease Control and Prevention ( CDC) .

Few cases of the virulent H5N5 strain have been reported in mammals. This strain has been mainly found in wild birds in the Arctic, but last year it was also found in red foxes and lynx in Norway, otters in the Netherlands, lynxes in Finland, and red foxes, skunks, and raccoons in Canada. No cases of this strain in domestic animals have been reported to the World Organisation for Animal Health (WOAH) to date.

In recent years, WOAH has placed great emphasis on combating the spread of avian influenza and published on its website in December a call for all nations of the world to place greater emphasis on monitoring and actions to prevent the spread of dangerous avian influenza viruses.

The Icelandic Food and Veterinary Authority reiterates its recommendation to the public to report wild birds and wild mammals found dead, when the cause of death is not obvious. This is best done by registering a tip on the Icelandic Food and Veterinary Authority website. It is important to clearly describe the location, preferably by recording coordinates.

(...)

Source: MAST, https://www.mast.is/is/um-mast/frettir/frettir/fuglainfluensa-i-ketti

Highly pathogenic avian #H5N1 #influenza A virus #replication in ex vivo #cultures of #bovine mammary #gland and teat tissues

{Excerpt}

Our data indicate that bovine H5N1 viruses can replicate efficiently in the epithelium of the bovine teat cistern, suggesting that they invade the mammary gland through the teat canal, which is more easily accessed by viruses. H5N1 virus is thought to be transmitted among lactating dairy cattle through contaminated milking equipment and/or milker's hands during milking[23]. Proper milking procedures are required to prevent spread of HPAI H5N1 viruses in dairy cattle, thereby minimizing the risk of transmission from cows to other mammals including humans.

Source: Emerging Microbes and Infections, https://www.tandfonline.com/doi/full/10.1080/22221751.2025.2450029#d1e346

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