First Isolation and Characterization of #LiaoNing Virus from #Aedes Vexans #Mosquitoes in #Hokkaido, #Japan, in 2022
Abstract Background : The Liao ning virus (LNV), belonging to the genus Seadornavirus within the family Sedoreoviridae , is a mosquito-borne virus . It was originally isolated from Aedes dorsalis mosquitoes in China . The original LNV strain, LNVS-NE97-31, was reported to infect several mammalian cell lines and cause hemorrhagic symptoms in mice . Subsequently, another LNV strain, LNV NSW B115745, was isolated from Australian mosquitoes ; it was reported to exhibit insect-specific infection. Materials and Methods : Virus isolation was performed on mosquitoes collected in northern Hokkaido, Japan, in 2022 . The isolated virus was subjected to genomic and growth kinetics analyses. Results : A LNV strain was isolated from Aedes vexans mosquitoes . Genomic sequencing and phylogenetic analysis revealed the new strain as 22WN03, and it formed a robust clade with the original Chinese strain, LNVS-NE97-31. Growth kinetics analysis did not reveal any mammalian or avian cell lin...