ETIDIOH - NEW

  Highlights •  Respiratory tract bacterial colonization was highly prevalent among RSV-infected children. •  Moraxella catarrhalis colonization was significantly associated with mild RSV disease. •  Haemophilus influenzae carriage showed a trend toward increased severity. •  Household crowding independently correlated with severe RSV outcomes. •  Airway microbiota may modulate RSV clinical outcomes. Abstract Background Respiratory syncytial virus is a major cause of acute respiratory infection in children . While most cases are mild, some progress to life-threatening disease. The role of bacterial colonization in shaping respiratory syncytial virus outcomes remains incompletely understood. Objective To evaluate the association between respiratory tract bacterial colonization and respiratory syncytial virus disease severity in children. Study design Prospective cohort study conducted during 2019 and 2023. Children ≤24 months hospitalized with confirmed posi...

Abstract Cefotaxime-resistant Haemophilus influenzae (CRHI) are a global concern, but little is known about their molecular epidemiology . The goal of this study was to perform genomic profiling of 191 CRHI from Norway (n = 183) or Sweden (n = 8) (2006–2018) and assess clonal spread using core genome multilocus sequence typing (cgMLST)-based Life Identification Number (LIN) codes based on whole genome sequencing (Ion Torrent). Cefotaxime resistance was confirmed with broth microdilution minimal inhibitory concentration (MIC), interpreted with the European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints. 35.7% of isolates with cefotaxime gradient MIC of 0.25 mg/L were falsely resistant. All but two isolates (blood) were non-invasive, and all but two (serotype f) were non-typeable. Characterization included calling of resistance determinants, ftsI typing (penicillin-binding protein 3, PBP3), and classification of PBP3-mediated beta-lactam resistance (rPBP3), with a...