ABSTRACT Cell cultures are widely used to study infectious respiratory diseases and to test therapeutics; however, they do not faithfully recapitulate the architecture and complexity of the human respiratory tract . Lung organoids have emerged as an alternative model that partially overcomes this key disadvantage. Lung organoids can be cultured in various formats that offer potential for studying highly pathogenic viruses . However, the effects of these different formats on virus infection remain unexplored, leaving their relative value unclear. In this study, we generated primary lung organoids from human donor cells and used them to derive monolayers and air-liquid interface (ALI) cultures with the goal of comparing the replication kinetics of two circulating highly pathogenic coronaviruses , severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2 ) and Middle East respiratory syndrome coronavirus ( MERS-CoV ). Infection studies revealed that organoid-derived monolayers di...
Media Monitoring for Signals about Emerging Threats