ETIDIOH - NEW

ABSTRACT As companion animals, dogs are susceptible to various subtypes of influenza A virus (IAV), with the H3N2 and H3N8 subtypes of canine influenza virus (CIV) stably circulating among canines . Compared to the H3N8 CIV, the H3N2 CIV is more widely prevalent in canine populations and demonstrates increased adaptability to mammals , potentially facilitating cross-species transmission . Therefore, a comprehensive elucidation of the mechanisms underlying H3N2 CIV adaptation to mammals is imperative. In this study, we serially passaged the GD14-WT strain in murine lungs , successfully establishing a lethal H3N2 CIV infection model . From this model, we isolated the lethal strain GD14-MA and identified the key lethal mutations PA(S184N) and PB2(E627K). Moreover, the GD14-ma[PA(S184N)+PB2(E627K)] strain exhibited markedly enhanced pathogenicity in dogs . Viral titers in lung tissues from infected dogs and mice showed that GD14-ma[PA(S184N)+PB2(E627K)] does not increase its pathogenicity ...

Abstract Influenza A is a zoonotic disease that affects dogs, pigs, horses, poultry, and birds . In this report, a meta-analysis according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) was conducted. Studies of influenza A viruses in dogs providing prevalence or seroprevalence in any location worldwide were included. The results in this study show that the seroprevalence for the H3N8 influenza subtype was 7.96% (95% CI: 2.03–16.8, p < 0.001), for the H3N2 subtype was 7.44% (95% CI: 4.51–10.5, p < 0.001), and for the H1N1 subtype was 3.10% (95% CI: 0.890–6.01, p < 0.001). In the case of the prevalence analysis, a prevalence of 0.395% (95% CI: 0.160–2.44) for the H3N8 subtype. For the H3N2 subtype, a prevalence of 17.8% (95% CI: 6.66–32.6, p < 0.001) was found. No publication bias was observed in the studies evaluating seroprevalence in the H3N2 and H1N1 subtypes. In the H3N8 subtype, Begg’s test indicated publication bias, but Egger’s test sh...