Respiratory #Shedding of Infectious #SARS-CoV-2 #Omicron #XBB.1.41.1 Lineage among Captive White-Tailed #Deer, #Texas, #USA

Abstract

White-tailed deer (Odocoileus virginianus) have high value for research, conservation, agriculture, and recreation and might be key SARS-CoV-2 reservoirs. In November 2023, we sampled 15 female deer in a captive facility in Texas, USA. All deer had neutralizing antibodies to SARS-CoV-2; respiratory swab samples from 11 deer were SARS-CoV-2–positive by quantitative reverse transcription PCR, and 1 deer also had a positive rectal swab sample. Six of the 11 respiratory swab samples yielded infectious virus; replication kinetics of most samples displayed lower growth 24–48 hours postinfection in vitro than Omicron lineages isolated from humans in Texas in the same period. Virus growth was similar between groups by 72 hours, suggesting no strong attenuation of deer-derived virus. All deer viruses clustered in XBB Omicron clade and demonstrated more mutations than expected compared with contemporaneous viruses in humans, suggesting that crossing the species barrier was accompanied by a high substitution rate.

Source: Emerging Infectious Diseases Journal, https://wwwnc.cdc.gov/eid/article/31/2/24-1458_article

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An intranasal, NLC-delivered self-amplifying #RNA #vaccine establishes protective #immunity against pre-pandemic #H5N1 and #H7N9 #influenza

Abstract

Seasonal and pandemic influenzas are continuous threats to human health, requiring rapid development of vaccines to multiple evolving viral strains. New RNA vaccine technologies have the adaptability and manufacturability to facilitate pandemic preparedness but have limited flexibility in their route of administration, reducing the ability to establish local protective immune responses such as respiratory mucosal immunity. Here, we describe monovalent and bivalent self-amplifying RNA (saRNA) vaccines against A/Vietnam/1203/2004 H5N1 and A/Anhui/2013 H7N9. These saRNA vaccines express either H5 or H7 hemagglutinin and are formulated with a nanostructured lipid carrier (NLC) that permits both intramuscular (IM) and intranasal (IN) dosing. In mice, IM vaccination established systemic humoral and cellular responses but no detectable mucosal response, while IN administration induced robust systemic and mucosal immunity. The saRNA-NLC vaccines provided complete protection against morbidity and mortality in ferret challenge models, establishing this intranasally-administered saRNA-NLC vaccine platform as a potential pandemic response tool.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.07.631792v1?rss=1

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The Q226L #mutation can convert a highly pathogenic #H5 2.3.4.4e virus to #bind #human-type #receptors

Abstract

H5Nx viruses continue to wreak havoc in avian and mammalian species worldwide. The virus distinguishes itself by the ability to replicate to high titers and transmit efficiently in a wide variety of hosts in diverse climatic environments. Fortunately, transmission to and between humans is scarce. Yet, if such an event were to occur, it could spark a pandemic as humans are immunologically naive to H5 viruses. A significant determinant of transmission to and between humans is the ability of the influenza A virus hemagglutinin (HA) protein to shift from an avian-type to a human-type receptor specificity. Here, we demonstrate that a 2016 2.3.4.4e virus HA can convert to human-type receptor binding via a single Q226L mutation, in contrast to a cleavage-modified 2016 2.3.4.4b virus HA. Using glycan arrays, x-ray structural analyses, tissue- and direct glycan binding, we show that L133adelta and 227Q are vital for this phenotype. Thus, whereas the 2.3.4.4e virus HA only needs a single amino acid mutation, the modified 2.3.4.4b HA was not easily converted to human-type receptor specificity.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.10.632119v1?rss=1

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#USA, #Michigan: {Oakland County} Health Division: 11 people under health #surveillance after contact with #H5N1 #birdflu infected #poultry

Waterford Township, Mich. – The Oakland County Health Division is monitoring a situation involving highly pathogenic avian influenza (HPAI), commonly known as bird flu, identified in animals at the farm at Hess-Hathaway Park in Waterford Township. 

|--     Eleven individuals who had direct contact with the animals are being         monitored    for 10 days, beginning yesterday. --|

Initially, it was reported that two of the 11 are experiencing flu-like symptoms. Since then, the Health Division has learned that only one individual has symptoms. That person’s influenza test has been collected and results from the state lab are pending.

“The risk of contracting bird flu is very low for the general public, but it’s important to be aware of the disease in the community,” said Oakland County Director of Health and Human Services Leigh-Anne Stafford. “Protect yourself and prevent bird flu by avoiding direct contact with sick or dead birds and wash your hands thoroughly if you come into contact with them.

In response to the discovery of HPAI at the farm at Hess-Hathaway Park, Waterford Township has taken proactive measures. Supervisor Anthony Bartolotta emphasized the township’s commitment to safety.

“Until further notice, portions of the farm will be closed to protect our animals, staff and visitors. However, the rest of Hess-Hathaway Park remains open for community enjoyment,” Bartolotta said. “We appreciate the community’s cooperation, patience and understanding as we work to return our farm to regular operations. We look forward to reopening in the Spring of 2025.”

Residents are encouraged to follow these prevention tips:

-- Avoid direct contact with sick or dead birds and animals.

-- Use recommended personal protective equipment (PPE) if contact is necessary.

-- Refrain from touching surfaces contaminated by bird droppings or bodily fluids.

-- Avoid consuming raw milk or raw milk products.

-- If bird flu is suspected in a domestic flock, contact Michigan Department of Agriculture and Rural Development (MDARD) immediately at 800-292-3939 (daytime) or 517-373-0440 (after hours). Additionally, report cases of unusual or unexplained deaths among wild bird populations by contacting the Michigan Department of Natural Resources at 517-336-5030.

MDARD is closely monitoring and responding to reports of sick domestic birds and HPAI throughout the state.

Contact your health care provider if you’ve had close contact with domestic fowl or wild bird and have bird flu symptoms. 

Bird flu symptoms range from no symptoms to severe disease. Signs and symptoms of bird flu in people may include:

-- Eye redness and irritation (conjunctivitis)

-- Mild fever (100 degrees Fahrenheit or greater) or feeling feverish (fever may not always be present)

-- Cough

-- Sore throat

-- Runny or stuffy nose

-- Muscle or body aches

-- Headaches

-- Fatigue

-- Shortness of breath or difficulty breathing

-- Less common symptoms include diarrhea, nausea or vomiting.

HPAI virus is widespread in wild birds worldwide and detected in domestic poultry and other animals. It can spread in various ways from flock to flock, including by wild birds, through contact with infected animals, by equipment, and on the clothing and shoes of caretakers. 

More information about bird flu can be found on the Health Division’s website at oakgov.com/health or by contacting Nurse on Call at 800-848-5533 or noc@oakgov.com. Nurse on Call is available 8:30 a.m. to 5:00 p.m., Monday through Friday. For up-to-date public health information, follow @publichealthOC on Facebook and X.  

Read the initial alert from MDARD here. For additional bird flu information from the state, click on michigan.gov/birdflu.  

Source: Oakland County Department of Health, https://www.oakgov.com/Home/Components/News/News/1751/591

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National #ONEHEALTH #Framework to Address #Zoonotic #Diseases and Advance Public Health #Preparedness in the #USA

Executive Summary 

The first ever National One Health Framework to Address Zoonotic Diseases and Advance Public Health Preparedness in the United States (NOHF-Zoonoses), 2025-2029, establishes a structure to facilitate multisectoral and transdisciplinary coordination, collaboration, and communication across the federal government. 

Using the One Health approach, the framework addresses zoonotic diseases and other priority One Health issues in the United States (U.S.). 

The One Health approach recognizes the interdependence of the health of humans, domestic and wild animals, plants, and the wider environment (including ecosystems). 

This approach mobilizes multiple diverse sectors, disciplines, and communities to work together to promote well-being and address health and ecosystem threats. 

Previous multisectoral work in the U.S. identified the critical need to formalize federal One Health coordination to address zoonotic diseases and other One Health related issues across the U.S. Government. 

Therefore, in the 2023 Consolidated Appropriations Act and the 2021 House Appropriations Committee Report, Congress directed the Centers for Disease Control and Prevention (CDC) in coordination with other federal agencies, to develop a framework based on the One Health approach to address zoonotic diseases and advance public health preparedness. 

The Act also directed CDC to coordinate with the U.S. Department of Agriculture (USDA) and the Department of the Interior (DOI) to develop a mechanism to support coordination at the federal level related to prevention, detection, control, and response for zoonotic diseases and related One Health activities. 

The One Health approach applies to zoonotic diseases as well as many other health threats at the interconnection between people, animals, plants, and the environment.  

While the primary focus of the NOHF-Zoonoses is addressing zoonotic diseases and advancing public health preparedness, the U.S. OHCU has also incorporated other components of One Health into this framework. 

To meet these directives CDC partnered with DOI, USDA and other federal agencies beginning in November 2021, to plan the establishment of the United States One Health Coordination Unit (U.S. OHCU) and to draft the NOHFZoonoses. 

The U.S. OHCU was launched in January 2024, with joint leadership from CDC (Chair 2024-2026), DOI, and USDA.  

The U.S. OHCU is coordinated by a Chair that will rotate between CDC, USDA, and DOI on a biennial calendar year basis. 

U.S. OHCU membership includes 24 agencies related to health, agriculture, interior, wildlife, environment, development, state, commerce, defense, security, and other fields. 

The draft NOHF-Zoonoses was published in September 2023 via the Federal Register Notice process, to engage and obtain input from state, Tribe, local, and territorial (STLT), non-governmental partners, and the public.  

The NOHF-Zoonoses presents goals and objectives for application of the One Health approach to protect people, animals, and our shared environment in the U.S. from zoonotic diseases and advance public health preparedness to optimize health, food safety and security, and sustainability while also promoting biodiversity and conservation outcomes. 

The NOHF-Zoonoses was designed to align with and complement existing U.S. initiatives that incorporate the One Health approach. 

The seven goals outlined in the NOHF-Zoonoses include the following areas: 

-- (1) Coordination, Collaboration and Communication; 

-- (2) Prevention; 

-- (3) Preparedness; 

-- (4) Coordinated Outbreak Investigation, Response, and Recovery; 

-- (5) Surveillance; 

-- (6) Laboratory; and 

-- (7) Workforce. 

While this framework focuses on One Health coordination at the federal level, its success depends on robust partnerships with STLT, non-governmental organizations, academia, and private sector partners as well as collaboration with relevant international partners. 

Knowledge and best practices from the implementation of the NOHF-Zoonoses will inform future One Health priorities and strengthen the nation’s ability to address One Health threats and promote health, safety, security, and resilience at the human-animal-plant-environment interface. 

Advancing One Health collaboration in the U.S. through the U.S. OHCU and the NOHF-Zoonoses will enhance our ability to jointly prevent, detect, and respond to zoonotic disease threats and related One Health issues. This initiative will improve efficiency across the U.S. government by enhancing collaboration across all relevant sectors with governmental and non-governmental partners while optimizing resource use in order to protect the health, safety, and security of people, animals, plants, and our shared environment.

(...)

Source: US Centers for Disease Control and Prevention, https://www.cdc.gov/one-health/media/pdfs/2025/01/354391-A-NOHF-ZOONOSES-508_FINAL.pdf

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Modulation of #cytokeratin and #cytokine/chemokine expression following #influenza virus infection of differentiated #human #tonsillar epithelial cells

ABSTRACT

The tonsils have been identified as a site of replication for Epstein–Barr virus, adenovirus, human papillomavirus, and other respiratory viruses. Human tonsil epithelial cells (HTECs) are a heterogeneous group of actively differentiating cells. Here, we investigated the cellular features and susceptibility of differentiated HTECs to specific influenza viruses, including expression of avian-type and mammalian-type sialic acid (SA) receptors, viral replication dynamics, and the associated cytokine secretion profiles. We found that differentiated HTECs possess more abundant α2,3-linked SA (preferentially bound by avian influenza viruses) than α2,6-linked SA (preferentially bound by mammalian strains). This dual receptor expression suggests a role in influenza virus adaptation and tropism within the tonsils by facilitating the binding and entry of multiple influenza virus strains. Our results indicated the susceptibility of differentiated HTECs to a wide range of influenza viruses from human, swine, and avian hosts. Virus production for most strains was detected as early as 1 day post-infection (dpi), and typically peaked by 3 dpi. However, pandemic H1N1 virus showed remarkably delayed replication kinetics that did not peak until at least 7 dpi. Notably, influenza virus infection impacted the expression of cytokeratins in HTEC cultures, which correlated with altered cytokine secretion patterns. These patterns varied within the strains but were most distinct in swine H3N2 infection. In conclusion, differentiated HTECs exhibited a strain-specific pattern of influenza virus replication and innate immune responses that included changes in cytokeratin and cytokine expression. These studies shed light on the complex interplay between influenza viruses and host cells in the tonsils.

Source: Journal of Virology, https://journals.asm.org/doi/full/10.1128/jvi.01460-24?af=R

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#Cambodia records 1st #human #death from #H5N1 in 2025

PHNOM PENH, Jan. 10 (Xinhua) -- A 28-year-old man from southeast Cambodia's Kampong Cham province died of H5N1 human avian influenza on Friday, becoming the first death in 2025, the Ministry of Health said in a press statement.

"Laboratory results from the National Institute of Public Health showed on Jan. 9, 2025 that the man was positive for H5N1 virus," the statement said.

"Although our team of doctors had provided him intensive care, the patient died on Jan. 10, 2025 due to his severe condition, with the symptoms of fever, cough, and dyspnea," it added.

The patient lived in village 22 in Chamkar Leu district's Chamkar Andoung commune.

"According to queries, the patient raised and fed chicken, and he cooked sick chicken for food," the statement said.

Health authorities are looking into the source of the infection and are examining any suspected cases or people who have been in contact with the victim in order to prevent an outbreak in the community, it added.

H5N1 influenza is a flu that normally spreads between sick poultry, but it can sometimes spread from poultry to humans, and its symptoms include fever, cough, runny nose, and severe respiratory illness.

The Ministry of Health called on people to be extra vigilant and not to eat ill or dead poultry, saying that bird flu still poses a threat to people's health.

From 2003 to date, there were 73 cases of human infection with H5N1 influenza, including 44 deaths in the Southeast Asian country, according to the ministry.

Source: Xinhua, https://english.news.cn/asiapacific/20250110/b1035982821244e2828ae53394c1129f/c.html

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#Belgium - #Influenza A #H5N1 viruses of high pathogenicity (Inf. with) (non-poultry including wild birds) (2017-) - Immediate notification

 A wild sanderling in Oostende.

Source: WOAH, https://wahis.woah.org/#/in-review/6175

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#Human #Health #Surveillance During Animal #Disease #Emergencies: #Minnesota Department of Health Response to Highly Pathogenic Avian #Influenza Outbreaks, 2015 and 2022–2023

ABSTRACT

Objectives

Highly pathogenic avian influenza (HPAI) poses an occupational risk for poultry workers, responders, and others in contact with infected birds. The objective of this analysis was to describe HPAI surveillance methods and outcomes, and highlight the challenges, successes, and lessons learned during the Minnesota Department of Health’s (MDH’s) public health response to HPAI outbreaks in Minnesota poultry flocks in the years 2015 and 2022–2023.

Methods

During both outbreaks, MDH staff attempted to contact all potentially exposed people and conduct a standardized interview. People were considered exposed and at risk if they had entered a barn with poultry on any HPAI test-positive premises. With their consent, exposed persons were entered into illness monitoring until 10 days from their last exposure. In 2015, MDH monitored the health of poultry workers only. In the 2022–2023 response, MDH monitored the health of poultry workers, backyard flock owners, responders, and private contract workers. In 2022–2023, interview responses were entered into a REDCap (Research Electronic Data Capture) database in real time, which automatically entered the person into monitoring if they consented. Through REDCap, they received an automated email with a unique link to a short survey asking about any symptom development. Where appropriate, interview responses from poultry workers collected in 2015 were compared to interview responses from poultry workers collected in 2022–2023.

Results

From March 3 to June 5, 2015, MDH epidemiologists interviewed and evaluated 375 (86%) of 435 poultry workers from 110 HPAI-infected flocks. From March 25, 2022 through December 31, 2023, MDH epidemiologists interviewed and evaluated 649 (65%) of 992 poultry workers, responders, contractors, and backyard flock owners associated with 151 HPAI-infected flocks. Among poultry workers, self-reported personal protective equipment (PPE) usage declined significantly from 2015 to 2022–2023 (full PPE usage 51.8% vs. 23.9%, p < .01).

Conclusion

MDH’s long standing relationships with animal health officials and the poultry industry resulted in strong poultry worker participation rates in surveillance efforts during HPAI outbreaks in 2015 and 2022–2023. Self-reported PPE usage was low, particularly in 2022–2023. Improvements in PPE accessibility and technology are needed to protect workers and responders in the on-going HPAI outbreak.

Source: Journal of Agromedicine, https://www.tandfonline.com/doi/full/10.1080/1059924X.2024.2442406

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Testing of #Retail #Cheese, #Butter, Ice Cream, and Other Dairy #Products for Highly Pathogenic Avian #Influenza in the #US

Abstract

The recent outbreak of highly pathogenic avian influenza (HPAI) in dairy cows has created public health concerns about the potential of consumers being exposed to live virus from commercial dairy products. Previous studies support that pasteurization effectively inactivates avian influenza in milk and an earlier retail milk survey showed viral RNA, but no live virus could be detected in the dairy products tested. Because of the variety of products and processing methods in which milk is used, additional product testing was conducted to determine if HPAI viral RNA could be detected in retail dairy samples, and for positive samples by quantitative real-time RT-PCR (qRT-PCR) further testing for the presence of live virus. Revised protocols were developed to extract RNA from solid dairy products including cheese and butter. The solid dairy product was mechanically liquified with garnet and zirconium beads in a bead beater diluted 1–4 with BHI media. This preprocessing step was suitable in allowing efficient RNA extraction with standard methods. Trial studies were conducted with different cheese types with spiked-in avian influenza virus to show that inoculation of the liquified cheese into embryonating chicken eggs was not toxic to the embryos and allowed virus replication. A total of 167 retail dairy samples, including a variety of cheeses, butter, ice cream, and fluid milk were collected as part of a nationwide survey. A total of 17.4% (29/167) of the samples had detectable viral RNA by qRT-PCR targeting the matrix gene, but all PCR-positive samples were negative for live virus after testing with embryonating egg inoculation. The viral RNA was also evaluated by sequencing part of the hemagglutinin gene using a revised protocol optimized to deal with the fragmented viral RNA. The sequence analysis showed all viral RNA-positive samples were highly similar to previously reported HPAI dairy cow isolates. Using the revised protocols, it was determined that HPAI viral RNA could be detected in a variety of dairy products, but existing pasteurization methods effectively inactivate the virus assuring consumer safety.

Source: Journal of Food Protection, https://www.sciencedirect.com/science/article/pii/S0362028X24002151?via%3Dihub

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Detection of #antibodies against #H5 subtype highly pathogenic avian #influenza viruses in multiple #raccoons in Tokachi District, #Hokkaido, #Japan, from 2022 to 2023

Abstract

In recent years, infection cases of H5 subtype highly pathogenic avian influenza viruses (HPAIVs) in wild mammals have increased globally. To obtain recent epidemiological information regarding influenza A virus (IAV) infection in raccoons (Procyon lotor), the prevalence of anti-IAV antibodies in sera was analyzed among raccoons captured in Tokachi District, Hokkaido, Japan, from 2019 to 2023. Screening of serum samples using enzyme-linked immunosorbent assay and agar gel precipitation test detected anti-IAV antibodies in 5 of 114 (4.4 %) raccoons. All positive sera were from raccoons captured from 2022 to 2023. The hemagglutination inhibition test revealed that all five serum samples contained anti-H5 subtype HPAIV antibodies, and one also contained anti-H1 subtype antibodies. The neuraminidase inhibition test revealed that all five sera contained anti-N1 subtype antibodies, and one also contained anti-N8 subtype antibodies. In the virus neutralization test, these five sera showed stronger neutralization activity against the H5 subtype clade 2.3.4.4b HPAIV strain recently circulating worldwide compared to the old H5 HPAIV strain isolated in Japan in 2007. These findings suggested that raccoons could be involved in the circulation of H5 HPAIVs in nature.

Source: Virus Research, https://www.sciencedirect.com/science/article/pii/S0168170224002089?via%3Dihub

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Susceptibility of #bovine respiratory and mammary epithelial #cells to avian and #mammalian derived clade 2.3.4.4b #H5N1 highly pathogenic avian #influenza viruses

Abstract

Zoonotic transmission of avian influenza viruses into mammals is relatively rare due to anatomical differences in the respiratory tract between species. Recently, clade 2.3.4.4b highly pathogenic H5N1 avian influenza viruses were detected circulating in North American cattle. Sporadic transmission between cattle, humans, and other animals proximal to cattle or after consuming products from infected cattle has occurred, but thus far there is no evidence of human-to-human transmission. However, the virus has the potential to adapt to the mammalian respiratory tract with every transmission event that occurs, making it crucial to understand cellular and species tropism of the H5N1 2.3.4.4b viruses. We compared viral kinetics of clade 2.3.4.4b viruses isolated from birds and mammals in respiratory epithelial cells derived from cattle, human, swine, and ferret. We found that avian derived viruses could replicate in swine cells only, yet mammalian derived strains could replicate efficiently in all tracheal and nasal epithelial cells tested. Interestingly, only bovine mammary epithelial cells (MEC) and swine respiratory epithelial cells were permissive to both avian and mammalian derived strains, possibly due to increased sialic acid expression on bovine MEC compared to bovine tracheal epithelial cells (TEC). However, sialic acid expression differed between dairy and beef cows: TEC derived from a dairy cow had increased expression of alpha2,6;2,3 sialic acid receptors compared to TEC from a beef-dairy cow cross. This study highlights the ability of clade 2.3.4.4b H5N1 viruses derived from mammals but not wild birds to infect the respiratory epithelium of other mammalian hosts.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.09.632235v1?rss=1

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Asymptomatic #infection and #antibody #prevalence to co-occurring avian #influenza viruses vary substantially between sympatric #seabird species following #H5N1 #outbreaks

Abstract

Emerging infectious diseases are of major concern to animal and human health. Recent emergence of high pathogenicity avian influenza virus (HPAIV) (H5N1 clade 2.3.4.4b) led to substantial global mortality across a range of host species. Co-occurring species showed marked differences in mortality, generating an urgent need for better epidemiological understanding within affected populations. We therefore tested for antibodies, indicative of previous exposure and recovery, and for active viral infection in apparently healthy individuals (n = 350) across five co-occurring seabird species on the Isle of May, Scotland, during 2023, following H5N1 HPAIV associated mortality in the preceding summer. Antibody prevalence to AIV subtypes varied substantially between species, ranging from 1.1% in European shags (Gulosus aristotelis) (to H5) to 78.7% in black-legged kittiwakes (Rissa tridactyla) (to H16 or both H13 and H16), and between 31 and 41% for three auk species (H5, H16 or both). At least 20.4% of auks had antibodies to an as yet unidentified subtype, suggesting further subtypes circulating in the population. We found low levels of active, but asymptomatic, AIV infection in individuals (1.6–4.5%), but excluded this as H5N1. Our results emphasise the importance of testing healthy individuals to understand the prevalence of co-circulating AIV subtypes in wild populations, and the potential for future reassortment events which could alter virus behaviour and impact.

Source: Scientific Reports, https://www.nature.com/articles/s41598-025-85152-6

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#Iceland, Avian #influenza #H5N5 in #cats

 {Original text in Icelandic, translated, edited.}

The Icelandic University of Iceland's Pathology Laboratory at Keldur notified the Icelandic Food and Veterinary Authority on January 6 that a kitten that arrived at the laboratory for autopsy had been diagnosed with a severe strain of avian influenza (H5N5). 

This is the same strain that has been detected in wild birds in Iceland since September last year and on one poultry farm in early December. 

The Icelandic Food and Veterinary Authority immediately issued instructions for disease control to prevent the spread of the infection and is now working to trace the infection. 

Symptoms of the disease in this cat included loss of appetite, weakness, stiffness, tremors, seizures and other neurological symptoms. 

Cat owners are asked to contact a veterinarian immediately if they notice such symptoms in their cats.

The cat diagnosed with bird flu was a 10-week-old kitten that died on December 22. The littermate the kitten was from and another kitten from the same litter died after a short illness two days earlier. They were not tested. 

The kitten's other littermates had left the home before the illness occurred and are all asymptomatic today. 

The cats are from Ísafjörður, but the kitten diagnosed with the infection had arrived in Reykjavík. The owners of all the cats have been contacted.

The Icelandic Food and Veterinary Authority believes that it is most likely that the cats were infected by an infected wild bird. 

At present, there is no evidence of infection in more cats, but the Icelandic Food and Veterinary Authority asks cat owners and veterinarians to be on the lookout for symptoms that may indicate avian influenza infection. 

There have been a number of diagnoses in wild birds in recent months, and therefore there is some risk that cats can become infected while hunting or from carcasses they come across. 

However, the Icelandic Food and Veterinary Authority does not believe the risk is so great that there is reason to warn against letting cats outside. 

People are, however, reminded to always maintain general hygiene when interacting with animals and caring for them. 

General information about avian influenza and guidelines for disease prevention can be found on the website of the Directorate of Health . 

It is worth noting that the risk of infection for people caused by the avian influenza virus is low, according to information on the website of the European Centre for Disease Prevention and Control (ECDC) .

In recent years, avian influenza has been increasingly detected in various species of mammals around the world. The most common type is the highly pathogenic H5N1 strain. This trend clearly demonstrates the virus's ability to adapt to new animal species. One of the greatest concerns worldwide is the current outbreak of avian influenza in dairy cows in the United States caused by the highly pathogenic H5N1 strain. However, the genotype of the virus in question has not yet been identified anywhere else in the world. Detailed information about this can be found on the websites of the United States Department of Agriculture USDA and the United States Centers for Disease Control and Prevention ( CDC) .

Few cases of the virulent H5N5 strain have been reported in mammals. This strain has been mainly found in wild birds in the Arctic, but last year it was also found in red foxes and lynx in Norway, otters in the Netherlands, lynxes in Finland, and red foxes, skunks, and raccoons in Canada. No cases of this strain in domestic animals have been reported to the World Organisation for Animal Health (WOAH) to date.

In recent years, WOAH has placed great emphasis on combating the spread of avian influenza and published on its website in December a call for all nations of the world to place greater emphasis on monitoring and actions to prevent the spread of dangerous avian influenza viruses.

The Icelandic Food and Veterinary Authority reiterates its recommendation to the public to report wild birds and wild mammals found dead, when the cause of death is not obvious. This is best done by registering a tip on the Icelandic Food and Veterinary Authority website. It is important to clearly describe the location, preferably by recording coordinates.

(...)

Source: MAST, https://www.mast.is/is/um-mast/frettir/frettir/fuglainfluensa-i-ketti

Highly pathogenic avian #H5N1 #influenza A virus #replication in ex vivo #cultures of #bovine mammary #gland and teat tissues

{Excerpt}

Our data indicate that bovine H5N1 viruses can replicate efficiently in the epithelium of the bovine teat cistern, suggesting that they invade the mammary gland through the teat canal, which is more easily accessed by viruses. H5N1 virus is thought to be transmitted among lactating dairy cattle through contaminated milking equipment and/or milker's hands during milking[23]. Proper milking procedures are required to prevent spread of HPAI H5N1 viruses in dairy cattle, thereby minimizing the risk of transmission from cows to other mammals including humans.

Source: Emerging Microbes and Infections, https://www.tandfonline.com/doi/full/10.1080/22221751.2025.2450029#d1e346

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#Philippines - High pathogenicity avian #influenza #H5N2 viruses (#poultry) (Inf. with) - Immediate notification

Backyard reared poultry in Camarines Norte Region.

Source: WOAH, https://wahis.woah.org/#/in-review/6167

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#Iceland - #Influenza A #H5N5 viruses of high pathogenicity (Inf. with) ({household #cats}) (2017-) - Immediate notification [FINAL]

Three cats (one adult female and two 10 week old kittens) from the same household died on December 20., 21. and 22. after they had been ill for only a few days. The main clinical signs were lethargy, anorexia, cramps and stiffness. Other kittens from the same litter had been rehomed before those three got sick and they have not shown any symptoms. The most probable source of infection is wild birds. HPAI H5N5 have been diagnosed in a few wild birds in Iceland since September last year.

Source: WOAH, https://wahis.woah.org/#/in-review/6168

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#Greenland - #Influenza A #H5N5 viruses of high pathogenicity (Inf. with) (non-poultry including wild birds) (2017-) - Immediate notification

 A wild Northern Fulmar in Danish Exclusive Economic Zone (Greenland).

Source: WOAH, https://wahis.woah.org/#/in-review/6172

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#Evidence of avian and human #influenza A virus #infection in farmed Siamese #crocodiles (Crocodylus siamensis) in #Thailand

Abstract

Crocodilians are susceptible to a range of virus infection including influenza A virus (IAV). However, little is known about the ecology and epidemiology of IAV in crocodile species. This study aimed to investigate IAV infection in farmed Siamese crocodiles in central Thailand. We collected plasma samples and pharyngeal swab samples from Siamese crocodiles residing in 13 crocodile farms in 9 provinces of central Thailand during 2019. Additional archival plasma samples of Siamese crocodiles collected in 2012 and 2018 were also included in the study. Plasma samples were screened for influenza A antibodies by a hemagglutination inhibition (HI) assay and positive were evaluated by a cytopathic effect/hemagglutination based-microneutralization (MN) assay. Swab samples were tested for influenza viral RNA by a real-time RT-PCR assay targeting the influenza matrix (M) gene. Among 246 tested plasma samples, the overall seroprevalence of antibodies against IAV in farmed Siamese crocodiles was 17.5% (43/246). The most common hemagglutinin (HA) subtype was H2 (46.5%, 20/43) followed by H9 (39.5%, 17/43), human H1 (14%, 6/43) and H1 (7%, 3/43). Multiple HA subtypes were also detected in 7% (3/43) of infected crocodiles with combination of H1 and H2 subtypes. All 126 tested swab samples were negative for influenza viral RNA. In addition, we demonstrated the ability of wild-type IAV subtypes (H1, H2, H9 and human H1) to infect primary Siamese crocodile fibroblast cells. To our knowledge, this is the first report of serological evidences of avian and human IAV infection in Siamese crocodiles. Our findings highlighted the role of crocodile species in the ecology of IAV particularly the potential to serve as the reservoir or mixing vessel for the viruses that significantly threaten both human and animal health.

Source: PLoS One, https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0317035

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Long-term immune responses induced by low-dose #infection with high pathogenicity avian #influenza viruses can protect #mallards from reinfection with a heterologous strain

Abstract

Migratory water birds are considered to be carriers of high pathogenicity avian influenza viruses (HPAIVs). In Japan, mallards are often observed during winter, and HPAIV-infected mallards often shed viruses asymptomatically. In this study, we focused on mallards as potential carriers of HPAIVs and investigated whether individual wild mallards are repeatedly infected with HPAIVs and act as HPAIV carriers multiple times within a season. Mallards were experimentally infected with H5N1 and H5N8 HPAIVs that were isolated recently in Japan and phylogenetically belong to different hemagglutinin groups (G2a, G2b, and G2d). All of these strains are more infectious to mallards than to chickens, and the infected mallards shed enough virus to infect others, regardless of whether they exhibited clinical signs. Serum antibodies to the homologous antigen, induced by a single infection with a low virus dose (10 times the 50% mallard infectious dose), were maintained at detectable levels for 84 days. Immunity at 84 days post-inoculation fully protected the mallards from a challenge with the homologous strain, as demonstrated by a lack of viral shedding, and antibody levels did not increase significantly in most of these birds. Protection against heterologous challenge was also observed despite undetectable levels of antibodies to the challenge strain. Our findings suggest that repeated infections with homologous and heterologous HPAIV strains do not occur frequently in individual wild mallards within a season, particularly at low viral doses, and the frequency with which they act as carriers may be limited.

Source: Archives of Virology, https://link.springer.com/article/10.1007/s00705-024-06209-x

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#Comparison of Contemporary and Historic Highly Pathogenic Avian #Influenza A(#H5N1) Virus #Replication in #Human #Lung #Organoids

Abstract

We compared virus replication and host responses in human alveolar epithelium infected with highly pathogenic avian influenza (HPAI) A(H5N1) viruses. A/Vietnam/1203/2004 replicated most efficiently, followed by A/Texas/37/2024, then A/bovine/Ohio/B24OSU-342/2024. Induction of interferon-stimulated genes was lower with A/Texas/37/2024 and A/bovine/Ohio/B24OSU-342/2024, which may indicate a reduced disease severity of those viruses.

Source: Emerging Infectious Diseases Journal, https://wwwnc.cdc.gov/eid/article/31/2/24-1147_article

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#Information for #Workers Exposed to #H5N1 #Birdflu {virus}

 {Summary}

At a glance

-- H5N1 bird flu is caused by viruses that can infect cows and other animals but most commonly affect birds and poultry. 

-- People who work with infected animals or their byproducts (for example, raw milk), might get sick from the virus. 

-- For example, dairy and poultry workers might get sick with H5N1 bird flu. 

-- Your employer should develop a workplace health and safety plan and share it with you. 

-- CDC updated this page to add information about worker exposure levels (low, medium, and high). 

-- We also added detail about how to use the right personal protective equipment safely for each exposure level.

(...)

Source: US Centers for Disease Control and Prevention, https://www.cdc.gov/bird-flu/prevention/farm-workers.html

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#Surveillance of Highly Pathogenic Avian #Influenza Virus in Wild #Canids from #Pennsylvania, #USA

Abstract

The avian influenza virus is a global pathogen with significant health and economic implications. While primarily a pathogen of wild and domestic birds, recent outbreaks of the H5N1 highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4b have caused mortality in a wide variety of mammals, including members of the Canidae family, on multiple continents. Despite sporadic mortality events globally, the epidemiology and pathobiology of H5N1 HPAIV in wild canids remains poorly defined. During 2022–2024, 41 wild canid carcasses (diagnostic cases), including 23 red foxes and 18 gray foxes, were tested for the influenza A virus (IAV) via PCR, with five red fox kits testing positive (12%). Infected animals had variably severe encephalitis, pneumonia, and occasionally myocarditis associated with strong immunolabeling for IAV. Serum from 269 wild canids in Pennsylvania was tested for antibodies to IAV, including 133 samples collected prior to 2021 (pre-H5N1 HPAIV 2.3.4.4b introduction) and 136 collected after 2022 (post-H5N1 HPAIV 2.3.4.4b introduction). All samples collected prior to 2021 were seronegative for IAV. Two coyotes from 2024 were seropositive for IAV but were negative for antibodies to the H5 and N1 subtypes. Collectively, these data suggest that while sporadic H5N1 HPAIV infection and mortality can occur in wild canids, particularly juvenile red foxes, infection was limited in these outwardly healthy and opportunistically sampled animals. Future studies should utilize a risk-based approach to target sampling of wild canids at increased risk for H5N1 HPAIV infection, such as those around waterfowl habitats or spatially around wild bird or domestic animal outbreaks.

Source: Animals, https://www.mdpi.com/2076-2615/14/24/3700

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#Analysis of the Monophyletic #Lineage of Avian #Influenza #H5N1 Which Circulated in #Venezuelan #Birds During the 2022–2023 #Outbreak

Abstract

Avian influenza subtype H5N1 has caused outbreaks worldwide since 1996, with the emergence of the Guandong lineage in China. The current clade 2.3.4.4b has evolved from this lineage, with increased virulence and mass mortality events in birds and mammals. The objective of this study was the analysis of 17 viral genomes of H5N1 avian influenza isolated in Venezuela during the 2022–2023 outbreak. The eight viral genomic segments were amplified using universal primers and sequenced via next-generation sequencing. The sequences were analyzed to confirm the H5 hemagglutinin clade, identify possible genetic reassortments, and perform a phylogenetic and docking analysis of the viral isolates. The viruses found in Venezuela belonged, as expected, to clade 2.3.4.4b and formed a monophyletic clade with North American influenza viruses, with no evidence of further reassortment. The introduction of the virus in South America is associated with bird migration through the Atlantic (Venezuela), Atlantic/Mississippi (Choco, Colombia), and Pacific migratory flyways, with the emergence of several viral lineages. Several mutations were found in all segments of the genome, although none of the key mutations was involved in mammalian adaptation. Moreover, in silico structural analysis suggests, as expected, that the viral hemagglutinin maintained a predilection for avian α2,3-linked sialic acid. The unprecedented pathogenic outbreak of avian influenza disease in South America was associated with the circulation of three different lineages, which maintain a lower affinity for the mammalian receptor.

Source: Microorganisms, https://www.mdpi.com/2076-2607/12/12/2519

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#Genetic and #Molecular Characterization of Avian #Influenza A(#H9N2) Viruses from Live #Bird #Markets (LBM) in #Senegal

Abstract

Despite extensive experience with influenza surveillance in humans in Senegal, there is limited knowledge about the actual situation and genetic diversity of avian influenza viruses (AIVs) circulating in the country, hindering control measures and pandemic risk assessment. Therefore, as part of the “One Health” approach to influenza surveillance, we conducted active AIV surveillance in two live bird markets (LBMs) in Dakar to better understand the dynamics and diversity of influenza viruses in Senegal, obtain genetic profiles of circulating AIVs, and assess the risk of emergence of novel strains and their transmission to humans. Cloacal swabs from poultry and environmental samples collected weekly from the two LBMs were screened by RT-qPCR for H5, H7, and H9 AIVs. Subsequently, a subset of H9-positive samples was selected for whole sequencing. From December 2023 to October 2024, 499 samples were tested, and AIV was detected in 58.3% of them. Among these, A/H9N2 was the only subtype detected in both markets, with a detection rate of 47.7% (82/172) in Thiaroye and 35.3% (42/119) in Tilene, resulting in an overall positivity rate of 42.6% (124/291). Genome sequencing of 22 A/H9N2 isolates, including 11 poultry drinking water samples, 7 carcass wash water samples, 3 fecal samples, and 1 cloacal swab, yielded 7 complete and 15 partial genomic sequences. Phylogenetic analyses of the resulting sequences showed that the A/H9N2 isolates obtained in this study formed a monophyletic cluster and were closely related to the Senegalese human strain (A/Senegal/0243/2019) identified through the national influenza sentinel surveillance program. These strains were also closely related to the A/H9N2 viruses of the G1 lineage circulating in neighboring countries, suggesting cross-border transmission. The A/H9N2 strains carried the low pathogenicity RSSR/GLF motif at the HA cleavage site and possessed several key amino acid mutations, including HA-I155T and HA-Q226L, which are associated with human host adaptation, PB2-T105V, PB2-A661T, and PB2-A588V, which are linked to the human-to-human transmission and increased polymerase activity, NS2-T14M, NS2-M100I, NS1-I106M, NS1-V222M, NS1-E223A, NS1-I226V, NS1-E227G, and NS1-P228S, which are known to alter virulence (increased or reduced) in humans or mice, and M2-S31N, which promotes drug resistance. Seven potential N-glycosylation sites were predicted in the HA protein and six in the NA protein. The selection pressure analysis revealed that the A/H9N2 isolates were primarily under neutral evolution or purifying selection pressure. Overall, our findings highlight the potential for cross-species transmission of Senegalese A/H9N2 viruses, emphasizing the need for sustained monitoring of these viruses in both animal and human populations.

Source: Viruses, https://www.mdpi.com/1999-4915/17/1/73

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#Disruption of seasonal #influenza #circulation and #evolution during the 2009 #H1N1 and #COVID19 #pandemics in Southeastern #Asia

Abstract

East, South, and Southeast Asia (together referred to as Southeastern Asia hereafter) have been recognized as critical areas fuelling the global circulation of seasonal influenza. However, the seasonal influenza migration network within Southeastern Asia remains unclear, including how pandemic-related disruptions altered this network. We leveraged genetic, epidemiological, and airline travel data between 2007-2023 to characterise the dispersal patterns of influenza A/H3N2 and B/Victoria viruses both out of and within Southeastern Asia, including during perturbations by the 2009 A/H1N1 and COVID-19 pandemics. During the COVID-19 pandemic, consistent autumn-winter movement waves from Southeastern Asia to temperate regions were interrupted for both subtype/lineages, however the A/H1N1 pandemic only disrupted A/H3N2 spread. We find a higher persistence of A/H3N2 than B/Victoria circulation in Southeastern Asia and identify distinct pandemic-related disruptions in A/H3N2 antigenic evolution between two pandemics, compared to interpandemic levels; similar patterns are observed in B/Victoria using genetic distance. The internal movement structure within Southeastern Asia markedly diverged during the COVID-19 pandemic season, and to a lesser extent, during the 2009 A/H1N1 pandemic season. Our findings provide insights into the heterogeneous impact of two distinct pandemic-related disruptions on influenza circulation, which can help anticipate the effects of future pandemics and potential mitigation strategies on influenza dynamics.

Source: Nature Communications, https://www.nature.com/articles/s41467-025-55840-y

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#Trends of acute respiratory #infection, including human #metapneumovirus, in the Northern Hemisphere

Situation at a glance

In many countries of the Northern Hemisphere, trends in acute respiratory infections increase at this time of year. 

These increases are typically caused by seasonal epidemics of respiratory pathogens such as seasonal influenza, respiratory syncytial virus (RSV), and other common respiratory viruses, including human metapneumovirus (hMPV), as well as mycoplasma pneumoniae. 

Many countries conduct routine surveillance for acute respiratory infections and common respiratory pathogens. 

Currently, in some countries in the temperate Northern hemisphere, influenza-like illness (ILI) and/or acute respiratory infection (ARI) rates have increased in recent weeks and are above baseline levels, following usual seasonal trends. 

Seasonal influenza activity is elevated in many countries in the Northern hemisphere. 

Where surveillance data is available, trends in RSV detections currently vary by region with decreases reported in most regions except in North America. 

Recently, there has been interest in hMPV cases in China including suggestions of hospitals being overwhelmed. 

hMPV is a common respiratory virus found to circulate in many countries in winter through to spring, although not all countries routinely test and publish data on trends in hMPV . 

While some cases can be hospitalized with bronchitis or pneumonia, most people infected with hMPV have mild upper respiratory symptoms similar to the common cold and recover after a few days. 

Based on data published by China, covering the period up to 29 December 2024, acute respiratory infections have increased during recent weeks and detections of seasonal influenza, rhinovirus, RSV, and hMPV, particularly in northern provinces of China have also increased. 

The observed increase in respiratory pathogen detections is within the range expected for this time of year during the Northern hemisphere winter. 

In China, influenza is the most commonly detected respiratory pathogen currently affecting people with acute respiratory infections. 

WHO is in contact with Chinese health officials and has not received any reports of unusual outbreak patterns. 

Chinese authorities report that the health care system is not overwhelmed and there have been no emergency declarations or responses triggered. 

WHO continues to monitor respiratory illnesses at global, regional and country levels through collaborative surveillance systems, and provides updates as needed.

Description of the situation

In many countries of the Northern Hemisphere, trends in acute respiratory infections increase at this time of year. These increases are typically caused by seasonal epidemics of respiratory pathogens such as seasonal influenza, RSV, and other common respiratory viruses, including hMPV, as well as mycoplasma pneumoniae. The co-circulation of multiple respiratory pathogens during the winter season can sometimes cause an increased burden on health care systems treating sick persons.

Currently, in some countries in the temperate Northern hemisphere, influenza-like illness (ILI) and/or acute respiratory infection (ARI) rates have increased in recent weeks and are above baseline levels, following usual seasonal trends. 

Influenza activity is elevated in many countries in Europe, Central America and the Caribbean, Western Africa, Middle Africa, and many countries across Asia, with the predominant seasonal influenza type and subtype varying by location, typical for this time of year, except during most of 2020 and 2021, when there was little influenza activity during the COVID-19 pandemic (...). 

SARS-CoV-2 activity as detected in sentinel surveillance and reported to Global Influenza Surveillance and Response System (GISRS), along with wastewater monitoring from the reporting countries, is currently low in countries in the Northern hemisphere following prolonged high level activity during summer months in the Northern hemisphere. 

Where surveillance data is available, trends in RSV activity are variable by region with downward trends observed in most subregions of the Americas, except in North America where RSV activity has increased, and decreases have been observed in the European region in recent weeks. Some countries conduct routine surveillance and report trends for other commonly circulating respiratory pathogens, such as hMPV, and report such information on a routine basis. Some countries in the Northern hemisphere have reported increased trends, varying by virus, in recent weeks, typical for this time of year.  

There has been international interest in a potential increase of respiratory virus transmission in China, particularly hMPV, including suggestions of hospitals being overwhelmed. China has an established sentinel surveillance system for ILI and severe acute respiratory infections (SARI), including hMPV, and conducts routine virological surveillance for common respiratory pathogens with detailed reports published weekly on the China Center for Disease Control and Prevention (CDC) website.[1] Surveillance and laboratory data for hMPV is not available routinely from all countries.

According to the most recent surveillance data on acute respiratory infections shared by the China CDC with data up to 29 December 2024, there has been an upward trend of common acute respiratory infections, including those due to seasonal influenza viruses, RSV and hMPV – as expected for this time of year during the Northern Hemisphere winter. 

Influenza is currently the most reported cause of respiratory disease, with the highest positivity rate among all monitored pathogens for all age groups except children aged 5-14 years for whom mycoplasma pneumoniae had the highest positivity rate. SARS-CoV-2 activity remains low however with an increase in reported severe COVID-19 cases. 

The predominant circulating SARS-CoV-2 variant in the country is XDV and its sublineages accounting for  59.1% detection among sequenced samples. ILI activity in China’s northern and southern provinces have been increasing since late 2024, following the previous year’s trends. Current ILI activity in the southern provinces remains below that of the previous two years, while current ILI activity in the northern provinces is similar to levels seen at this time in the previous two years.

China’s reported levels of acute respiratory infections, including hMPV, are within the expected range for the winter season with no unusual outbreak patterns reported. Chinese authorities confirmed that the health care system is not overwhelmed, hospital utilization is currently lower than this time last year, and there have been no emergency declarations or responses triggered. Since the expected seasonal increase was observed, health messages have been provided to the public on how to prevent the spread of respiratory infections and reduce the impact of these diseases.

Public health response

Based on the expected increase in respiratory infections during the winter season, countries, including China, have been providing health messages to the public on how to prevent the spread of respiratory infections and reduce the impact of disease.

WHO risk assessment

In temperate climates, seasonal epidemics of common respiratory pathogens, including influenza, occur often during winter periods. The observed increases in acute respiratory infections and associated pathogen detections in many countries in the Northern hemisphere in recent weeks is expected at this time of year and is not unusual. The co-circulation of respiratory pathogens may pose a burden to health facilities. 

WHO advice

WHO recommends that individuals in areas where it is winter take normal precautions to prevent the spread and reduce risks posed by respiratory pathogens, especially to the most vulnerable. People with mild symptoms should stay home to avoid infecting other people and rest. 

People at high risk or with complicated or severe symptoms should seek medical care as soon as possible. 

Individuals should also consider wearing a mask in crowded or poorly ventilated spaces, cover coughs and sneezes with a tissue or bent elbow, practice regular handwashing, and get recommended vaccines as per physician and local public health authorities’ advice.[2]

WHO advises Member States to maintain surveillance for respiratory pathogens through an integrated approach, considering country context, priorities, resources and capacities. WHO has published guidance on integrated surveillance here. WHO has also updated guidance on assessing influenza epidemic and pandemic severity, including the impact on healthcare facilities, here.

Based on the current risk assessment, WHO advises against any travel or trade restrictions related to current trends in acute respiratory infections.

Further information

-- World Health Organization (WHO). Implementing the integrated sentinel surveillance of influenza and other respiratory viruses of epidemic and pandemic potential by the Global Influenza Surveillance and Response System. Available at: https://iris.who.int/handle/10665/379678

-- WHO fact sheet for Influenza (Seasonal): https://www.who.int/news-room/fact-sheets/detail/influenza-(seasonal)

-- WHO Routine influenza weekly updates. Available at:https://www.who.int/teams/global-influenza-programme/surveillance-and-monitoring/influenza-updates

-- WHO Influenza surveillance outputs. Available at: https://www.who.int/teams/global-influenza-programme/surveillance-and-monitoring/influenza-surveillance-outputs

-- WHO Global COVID-19 Dashboard. Available at:  https://data.who.int/dashboards/covid19/cases

-- WHO Coronavirus disease (COVID-19) Epidemiological Updates. Available at: https://www.who.int/emergencies/diseases/novel-coronavirus-2019/situation-reports

-- WHO Clinical practice guidelines for influenza. Available at: https://www.who.int/publications/i/item/9789240097759

-- WHO Respiratory Syncytial Virus (RSV) disease. Available at: https://www.who.int/teams/health-product-policy-and-standards/standards-and-specifications/norms-and-standards/vaccine-standardization/respiratory-syncytial-virus-disease https://www.chinacdc.cn/jksj/jksj04_14249/

-- US CDC Human Metapneumovirus. Available at: https://www.cdc.gov/human-metapneumovirus/about/index.html

-- American Lung Association. Human Metapneumovirus (hMPV) Symptoms and Diagnosis. Available at: https://www.lung.org/lung-health-diseases/lung-disease-lookup/human-metapneumovirus-hmpv/symptoms-diagnosis

[1] China CDC Weekly Influenza Surveillance Report. Available at: https://www.chinacdc.cn/jksj/jksj04_14249/   

[2] WHO Clinical practice guidelines for influenza. Available at: https://www.who.int/publications/i/item/9789240097759

(...)

Source: World Health Organization, https://www.who.int/emergencies/disease-outbreak-news/item/2025-DON550

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#Avian #flu #risk still ‘low’ after first #US #patient dies from #H5N1 virus: #WHO

7 January 2025 

A day after the United States reported its first human death from avian flu, the UN World Health Organization (WHO) insisted on Tuesday that the risk to the wider population remains “low”. 

WHO spokesperson Dr. Margaret Harris told reporters in Geneva that the H5N1 virus causing the disease is “not circulating in humans but jumping into humans” who are exposed to poultry or dairy cattle. “We’re not seeing sustained circulation,” she insisted.

Underlying conditions

The man who died of the disease in Louisiana was over 65 and reportedly had underlying medical conditions, Dr. Harris said. 

According to the health authorities, he had been exposed to chickens and wild birds. Several dozen people in the US have contracted avian influenza – commonly referred to as bird flu – during the current outbreak, mainly farmworkers in close contact with poultry flocks and cattle herds.

Dr. Harris stressed that WHO’s assessment of the risk to the general population “is still low and remains set”. The main concern is for people who work in animal industries because they need to be better protected from infection.

The WHO spokesperson added that the United States was continuing to carry out “a lot of surveillance” in the human and animal population, “in the methods we use for farming, for our food production…all those things need to be combined because indeed it always does pose a risk”.

China respiratory virus is not new

Meanwhile, a respiratory virus gaining ground in China, known as the human metapneumovirus, or hMPV, has been sparking media attention in recent weeks, but it does not represent a new or major threat, Dr. Harris insisted.

The UN health agency spokesperson said that such infections are on the rise in China “as expected during winter”, with seasonal influenza being “by far the most common among them”, as reported by the Chinese Center for Disease Control and Prevention.

“China’s reported levels of respiratory infections are within the usual range for the winter season,” Dr. Harris explained. “Authorities report that hospital utilization is currently lower than this time last year, and there have been no emergency declarations or responses triggered,” she added.

As for hMPV, it was first identified in 2001 and “has been in the human population for a long time”, Dr. Harris clarified. 

‘Very, very low’ risk

She added that it is a common virus that circulates in winter and spring and usually “causes respiratory symptoms similar to the common cold”. 

Like any of the hundreds of common cold viruses known to exist, it can lead to more serious disease in patients with low immunity, particularly but not limited to newborns and the elderly.

Asked about hMPV’s mortality rate, Dr. Harris described it as “very, very low”. It is not a pathogen that normally leads to deaths in humans, save for the most vulnerable, she concluded, recommending “simple” prevention measures, such as wearing a mask, improving ventilation of closed spaces and handwashing.

Source: United Nations, https://news.un.org/en/story/2025/01/1158776

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Social Media #Memes and Early Public #Opinion #Formation Regarding Highly Pathogenic Avian #Influenza (HPAI) as a Public Health #Threat in the #USA

 {Summary}

Misinformation, disinformation, and conspiracy theories gain traction in times of uncertainty when little is known about a disease and when trust in government and public institutions is low. Amidst uncertainty, public health-related memes act as a sort of coping mechanism. Memes images, text, or video that serve as a shorthand form of communication—have the potential to spread quickly and widely on social media. While memes can be humorous, perhaps harmless, they can have serious effects at other times, as during the COVID-19 pandemic. One thing they have in common, however, is their transportability from user to user and platform to platform.  Frame analysis of Internet memes is useful for categorizing what we call frames of meaning in digital space. Unlike the agenda-setting function of mass media, in which gatekeepers (e.g., news editors) set the agenda regarding public issues, framing theory in the digital age is more dynamic, as content may take on different forms or formats, change over time, and the digital frame may traverse media platforms. Consumer-generated content (CGC) plays a significant role in setting the agenda or framing the event. Based onthe fluid nature of social networks and changes in the hierarchy of agenda setting, the model of connective action applied to public discourse on digital media can be visualized and analyzed through topic modeling to understand the role that memes play in shaping emergent public issues. In this study, we focused on memes shared in response to the current Highly Pathogenic Avian Influenza (HPAI), specifically the H5N1 outbreak in the United States, to get a glimpse into the early stages of public opinion formation around a potential public health issue. We present this approach to study the situational awareness of this public health issue through analysis of memes as a form of public discourse and as a cost-effective tool for resource challenged public health agencies to scan the environment for emergent issues.

(...)

Source: Disaster Medicine and Public Health Preparedness, https://www.cambridge.org/core/journals/disaster-medicine-and-public-health-preparedness/article/social-media-memes-and-early-public-opinion-formation-regarding-highly-pathogenic-avian-influenza-hpai-as-a-public-health-threat-in-the-united-states/5119C8A5DA2152D4392FC93BDCE575A8

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#Polymerase #mutations underlie #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals.

Abstract

In early 2024, an unprecedented outbreak of H5N1 high pathogenicity avian influenza was detected in dairy cattle in the USA. The epidemic remains uncontrolled, with spillbacks into poultry, wild birds and other mammals including humans. Here, we present molecular and virological evidence that the cattle B3.13 genotype H5N1 viruses rapidly accumulated adaptations in polymerase genes that enabled better replication in bovine cells, as well as cells of other mammalian species including humans and pigs. We find evidence of several mammalian adaptations gained early in the evolution of these viruses in cattle including PB2 M631L, which is found in all cattle sequences, and PA K497R, which is found in the majority. Structurally, PB2 M631L maps to the polymerase-ANP32 interface, an essential host factor for viral genome replication. We show this mutation adapts the virus to co-opt bovine ANP32 proteins and thereby enhances virus replication in bovine and primary human airway cells. Importantly, we show that ongoing evolution during 2024 in the PB2 gene, including a convergently arising D740N substitution, further increases polymerase activity in a range of mammalian cells. Thus, the continued circulation of H5N1 in dairy cattle allows virus adaption improving replicative ability in cattle and increasing zoonotic spillover risk.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.01.06.631435v1?rss=1

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First #H5N1 #Birdflu #Death Reported in #USA

January 6, 2025 -- CDC is saddened by Louisiana's report that a person previously hospitalized with severe avian influenza A(H5N1) illness ("H5N1 bird flu") has passed away. 

While tragic, a death from H5N1 bird flu in the United States is not unexpected because of the known potential for infection with these viruses to cause severe illness and death. 

As of January 6, 2025, there have been 66 confirmed human cases of H5N1 bird flu in the United States since 2024 and 67 since 2022. 

This is the first person in the United States who has died as a result of an H5 infection. 

Outside the United States, more than 950 cases of H5N1 bird flu have been reported to the World Health Organization; about half of those have resulted in death.

CDC has carefully studied the available information about the person who died in Louisiana and continues to assess that the risk to the general public remains low. 

Most importantly, no person-to-person transmission spread has been identified. 

As with the case in Louisiana, most H5 bird flu infections are related to animal-to-human exposures. 

Additionally, there are no concerning virologic changes actively spreading in wild birds, poultry, or cows that would raise the risk to human health. (CDC reported previously on its analysis of the viruses isolated from the patient in Louisiana.) 

However, people with job-related or recreational exposures to infected birds or other animals are at greater risk of infection. For these and others, CDC has developed prevention resources about how to protect yourself.

CDC is continually:

-- Supporting critical epidemiologic investigations with state and local partners to assess the public health impact of each H5 case.

-- Working closely with state and local partners to conduct active surveillance for H5 cases.

-- Monitoring for changes that might suggest H5 viruses are becoming better adapted to mammals and therefore might spread more easily from animals-to-humans or human-to-human or cause more severe disease.

-- Monitoring for any viral changes that could make these viruses less responsive to flu antiviral medications or the available candidate vaccine viruses.

-- Additional information about H5 bird flu is available on the CDC website.

Source: US Centers for Disease Control and Prevention, https://www.cdc.gov/media/releases/2025/m0106-h5-birdflu-death.html

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#USA, #Louisiana Department of Health reports first U.S. #H5N1-related #human #death

{Edited}

January 06, 2025

The Louisiana Department of Health reports the patient who had been hospitalized with the first human case of highly pathogenic avian influenza (HPAI), or H5N1, in Louisiana and the U.S. has died. 

The patient was over the age of 65 and was reported to have underlying medical conditions. 

The patient contracted H5N1 after exposure to a combination of a non-commercial backyard flock and wild birds. 

LDH’s extensive public health investigation has identified no additional H5N1 cases nor evidence of person-to-person transmission. This patient remains the only human case of H5N1 in Louisiana. 

The Department expresses its deepest condolences to the patient’s family and friends as they mourn the loss of their loved one. Due to patient confidentiality and respect for the family, this will be the final update about the patient. 

While the current public health risk for the general public remains low, people who work with birds, poultry or cows, or have recreational exposure to them, are at higher risk.

The best way to protect yourself and your family from H5N1 is to avoid sources of exposure. That means avoiding direct contact with wild birds and other animals infected with or suspected to be infected with bird flu viruses. 

Protecting yourself and others from H5N1 infection

-- Do not touch sick or dead animals or their droppings and do not bring sick wild animals into your home.

-- Keep your pets away from sick or dead animals and their feces.

-- Do not eat uncooked or undercooked food. Cook poultry, eggs and other animal products to the proper temperature and prevent cross-contamination between raw and cooked food.

-- Avoid uncooked food products such as unpasteurized raw milk or cheeses from animals that have a suspected or confirmed infection.

-- If you work on poultry or dairy farms, talk to your provider about getting your seasonal flu vaccination. It will not prevent infection with avian influenza viruses, but it can reduce the risk of coinfection with avian and flu viruses.

-- Report dead or sick birds or animals to the USDA toll-free at 1-866-536-7593 or the Louisiana Department of Agriculture and Forestry Diagnostic Lab at 318-927-3441.

-- If you have been exposed to sick or dead birds or other animals or work on a farm where avian influenza has been detected, watch for respiratory symptoms or conjunctivitis. If you develop symptoms within 10 days after exposure to sick or dead animals, tell your healthcare provider that you have been in contact with sick animals and are concerned about avian influenza. This will help them give you appropriate advice on testing and treatment. Stay home and away from others while you have symptoms.

Source: Department of Health, https://ldh.la.gov/news/H5N1-death

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#USA reports its first #human fatal case of #H5N1 #birdflu virus

 {Excerpt, edited, original article in Bahasan}

KOMPAS.com - The United States recorded its first death from bird flu ( H5N1 ), as recorded by the Louisiana Department of Health (LDH), Monday (6/1/2025) local time. Quoted from the Washington Post , Tuesday (7/1/2025), this case occurred in Louisiana, involving a patient over 65 years old who had comorbidities.

(...)

Source: Kompas, https://health.kompas.com/read/25A07073728468/as-laporkan-kasus-kematian-pertama-flu-burung-pada-manusia#

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#USA, #Genetic #Sequences of Highly Pathogenic Avian #Influenza A(#H5N1) Viruses Identified in a Person in #Louisiana

Genetic Sequences of Highly Pathogenic Avian Influenza A(H5N1) Viruses Identified in a Person in Louisiana

What to know

-- CDC has sequenced the influenza viruses in specimens collected from the patient in Louisiana who was infected with, and became severely ill from HPAI A(H5N1) virus. 

-- The genomic sequences were compared to other HPAI A(H5N1) sequences from dairy cows, wild birds and poultry, as well as previous human cases and were identified as the D1.1 genotype. 

-- The analysis identified low frequency mutations in the hemagglutinin gene of a sample sequenced from the patient, which were not found in virus sequences from poultry samples collected on the patient’s property, suggesting the changes emerged in the patient after infection.

Background

This is a technical summary of an analysis of the genomic sequences of the viruses identified in two upper respiratory tract specimens from the patient who was severely ill from an infection with highly pathogenic avian influenza (HPAI) A(H5N1) virus in Louisiana. 

The patient was infected with A(H5N1) virus of the D1.1 genotype virus that is closely related to other D1.1 viruses recently detected in wild birds and poultry in the United States and in recent human cases in British Columbia, Canada, and Washington State. 

This avian influenza A(H5N1) virus genotype is different from the B3.13 genotype spreading widely and causing outbreaks in dairy cows, poultry, and other animals, with sporadic human cases in the United States. 

Deep sequencing of the genetic sequences from two clinical specimens from the patient in Louisiana was performed to look for changes associated with adaptation to mammals. 

There were some low frequency changes in the hemagglutinin (HA) gene segment of one of the specimens that are rare in people but have been reported in previous cases of A(H5N1) in other countries and most often during severe infections. 

One of the changes found was also identified in a specimen collected from the human case with severe illness detected in British Columbia, Canada, suggesting they emerged during the clinical course as the virus replicated in the patient. 

Analysis of the N1 neuraminidase (NA), matrix (M) and polymerase acid (PA) genes from the specimens showed no changes associated with known or suspected markers of reduced susceptibility to antiviral drugs.

CDC Update

December 26, 2024 – CDC has sequenced the HPAI A(H5N1) avian influenza viruses in two respiratory specimens collected from the patient in Louisiana who was severely ill from an A(H5N1) virus infection. 

CDC received two specimens collected at the same time from the patient while they were hospitalized for severe respiratory illness: a nasopharyngeal (NP) and combined NP/oropharyngeal (OP) swab specimens. 

Initial attempts to sequence the virus from the patient's clinical respiratory specimens using standard RNA extraction and multisegment-RTPCR (M-RTPCR)1 techniques yielded only partial genomic data and virus isolation was not successful. 

Nucleic acid enrichment was needed to sequence complete genomes with sufficient coverage depth to meet quality thresholds. 

CDC compared the influenza gene segments from each specimen with A(H5N1) virus sequences from dairy cows, wild birds, poultry and other human cases in the U.S. and Canada. 

The genomes of the virus (A/Louisiana/12/2024) from each clinical specimen are publicly posted in GISAID (EPI_ISL_19634827 and EPI_ISL_19634828) and GenBank (PQ809549-PQ809564).

Summary of amino acid mixtures identified in the hemagglutinin (HA) of clinical specimens from the patient.

Overall, the hemagglutinin (HA) sequences from the two clinical specimens were closely related to HA sequences detected in other D1.1 genotype viruses, including viruses sequenced from samples collected in November and December 2024 in wild birds and poultry in Louisiana. 

The HA genes of these viruses also were closely related to the A/Ezo red fox/Hokkaido/1/2022 candidate vaccine virus (CVV) with 2 or 3 amino acid changes detected. 

These viruses have, on average, 3 or 4 amino acid changes in the HA when compared directly to the A/Astrakhan/3212/2020 CVV sequence. 

These data indicate the viruses detected in respiratory specimens from this patient are closely related to existing HPAI A(H5N1) CVVs that are already available to manufacturers, and which could be used to make vaccines if needed.

There were some differences detected between the NP/OP and the NP specimens. Despite the very close similarity of the D1.1 sequences from the Louisiana human case to bird viruses, deep sequence analysis of the HA gene segment from the combined NP/OP sample detected low frequency mixed nucleotides corresponding to notable amino acid residues (using mature HA sequence numbering):

 -- A134A/V [Alanine 88%, Valine 12%];

 -- N182N/K [Asparagine 65%, Lysine 35%]; and

 -- E186E/D [Glutamic acid 92%, Aspartic Acid 8%].

The NP specimen, notably, did not have these low frequency changes indicating they may have been detected from swabbing the oropharyngeal cavity of the patient. 

While these low frequency changes are rare in humans, they have been reported in previous cases of A(H5N1) in other countries and most often during severe disease2345. 

The E186E/D mixture, for example, was also identified in a specimen collected from the severe human case detected in British Columbia, Canada67.

This summary analysis focuses on mixed nucleotide detections at residues A134V, N182K, E186D as these changes may result in increased virus binding to α2-6 cell receptors found in the upper respiratory tract of humans. 

It is important to note that these changes represent a small proportion of the total virus population identified in the sample analyzed (i.e., the virus still maintains a majority of 'avian' amino acids at the residues associated with receptor binding). 

The changes observed were likely generated by replication of this virus in the patient with advanced disease rather than primarily transmitted at the time of infection. 

Comparison of influenza A(H5) sequence data from viruses identified in wild birds and poultry in Louisiana, including poultry identified on the property of the patient, and other regions of the United States did not identify these changes. 

Of note, virus sequences from poultry sampled on the patient's property were nearly identical to the virus sequences from the patient but did not have the mixed nucleotides identified in the patient's clinical sample, strongly suggesting that the changes emerged during infection as virus replicated in the patient. 

Although concerning, and a reminder that A(H5N1) viruses can develop changes during the clinical course of a human infection, these changes would be more concerning if found in animal hosts or in early stages of infection (e.g., within a few days of symptom onset) when these changes might be more likely to facilitate spread to close contacts. 

Notably, in this case, no transmission from the patient in Louisiana to other persons has been identified. 

The Louisiana Department of Public Health and CDC are collaborating to generate additional sequence data from sequential patient specimens to facilitate further genetic and virologic analysis.

Additional genomic analysis

The genetic sequences of the A(H5N1) viruses from the patient in Louisiana did not have the PB2 E627K change or other changes in polymerase genes associated with adaptation to mammals and no evidence of low frequency changes at critical positions. 

And, like other D1.1 genotype viruses found in birds, the sequences lack PB2 M631L, which is associated with viral adaptation to mammalian hosts, and which has been detected in >99% of dairy cow sequences but is only sporadically found in birds. 

Analysis of the N1 neuraminidase (NA), matrix (M) and polymerase acid (PA) genes from the specimens showed no changes associated with known or suspected markers of reduced susceptibility to antiviral drugs. 

The remainder of the genetic sequences of A/Louisiana/12/2024 were closely related to sequences detected in wild bird and poultry D1.1 genotype viruses, including poultry identified on the property of the patient, providing further evidence that the human case was most likely infected following exposure to birds infected with D1.1 genotype virus.

Follow Up Actions

Overall, CDC considers the risk to the general public associated with the ongoing U.S. HPAI A(H5N1) outbreak has not changed and remains low. The detection of a severe human case with genetic changes in a clinical specimen underscores the importance of ongoing genomic surveillance in people and animals, containment of avian influenza A(H5) outbreaks in dairy cattle and poultry, and prevention measures among people with exposure to infected animals or environments.

Source: US Centers for Disease Control and Prevention, https://www.cdc.gov/bird-flu/spotlights/h5n1-response-12232024.html

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#Zoonotic #transmission of novel #Influenza A #variant viruses detected in #Brazil during 2020 to 2023

Abstract

Zoonotic infections (swine-human) caused by influenza A viruses (IAVs) have been reported and linked to close contact between these species. Here, we describe eight human IAV variant infections (6 mild and 2 severe cases, including 1 death) detected in Paraná, Brazil, during 2020–2023. Genomes recovered were closely related to Brazilian swIAVs of three major lineages (1 A.3.3.2/pdm09, 1B/human-like, and H3.1990.5), including three H1N1v, two H1N2v, two H3N2v and one H1v. Five H1v were closely related to pdm09 lineage, one H1v (H1N2v) grouped within 1B.2.3 clade, and the two H3v grouped within a clade composed exclusively of Brazilian H3 swIAV (clade H3.1990.5.1). Internal gene segments were closely related to H1N1pdm09 isolated from pigs. IAV variant rarely result in sustained transmission between people, however the potential to develop such ability is of concern and must not be underestimated. This study brings into focus the need for continuous influenza surveillance and timely risk assessment.

Source: Nature Communications, https://www.nature.com/articles/s41467-024-53815-z

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#Portugal - High pathogenicity avian #influenza #H5N1 viruses (#poultry) (Inf. with) - Immediate notification

 A poultry farm in Lisboa Region.

Source: WOAH, https://wahis.woah.org/#/in-review/6164

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#Hungary - High pathogenicity avian #influenza #H5N1 viruses (#poultry) (Inf. with) - Immediate notification

 A poultry farm in Szabolcs-Szatmár-Bereg Region.

Source: WOAH, https://wahis.woah.org/#/in-review/6165

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#Germany - #Influenza A #H5N1 viruses of high pathogenicity (Inf. with) (non-poultry including wild birds) (2017-) - Immediate notification

 Wild Anatidae birds in Hessen Region, Frankfurt am Main.

Source: WOAH, https://wahis.woah.org/#/in-review/6166

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Anti- #neuraminidase and anti- #hemagglutinin #stalk responses to different #influenza A(#H7N9) #vaccine regimens

Abstract

Introduction

Pandemic influenza vaccine development focuses on the hemagglutinin (HA) antigen for potency and immunogenicity. Antibody responses targeting the neuraminidase (NA) antigen, or the HA stalk domain have been implicated in protection against influenza. Responses to the NA and HA-stalk domain following pandemic inactivated influenza are not well characterized in humans.

Material and methods

In a series of clinical trials, we determine the vaccines' NA content and demonstrate that NA inhibition (NAI) antibody responses increase in a dose-dependent manner following a 2-dose priming series with AS03-adjuvanted influenza A(H7N9) inactivated vaccine (A(H7N9) IIV). NAI antibody responses also increase with interval extension of the 2-dose priming series or following a 5-year delayed boost with a heterologous adjuvanted A(H7N9) IIV. Neither concomitant seasonal influenza vaccination given simultaneously or sequentially, nor use of heterologous A(H7N9) IIVs in the 2-dose priming series had an appreciable effect on NAI antibody responses. Anti-HA stalk antibody responses were minimal and not durable.

Conclusions

We provide evidence for strategies to improve anti-neuraminidase responses which can be further standardized for pandemic preparedness.

Source: Vaccine, https://www.sciencedirect.com/science/article/abs/pii/S0264410X24013719?via%3Dihub

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Evidence of #Influenza A(#H5N1) #Spillover #Infections in #Horses, #Mongolia

Abstract

Recent outbreaks of influenza A(H5N1) have affected many mammal species. We report serologic evidence of H5N1 virus infection in horses in Mongolia. Because H3N8 equine influenza virus is endemic in many countries, horses should be monitored to prevent reassortment between equine and avian influenza viruses with unknown consequences.

Source: Emerging Infectious Diseases Journal, https://wwwnc.cdc.gov/eid/article/31/1/24-1266_article

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