Emerging and re-emerging vector-borne and other #zoonotic #RNA #viruses: #pathogenesis, #climate-driven dynamics, and strategies for global control

 

Abstract

Vector-borne and other zoonotic RNA viruses provide a significant and growing threat to global health, especially in areas where climate change, urbanization, and population growth facilitate the proliferation of arthropod vectors. This review offers an extensive examination of the biology, epidemiology, and pathogenesis of numerous important viruses, including dengue, Zika, chikungunya, yellow fever, Japanese encephalitis, Crimean–Congo hemorrhagic fever, Nipah, Ebola, and hantaviruses. We underscore how environmental and social factors, particularly increasing temperatures, modified precipitation patterns, and accelerated urbanization, transform vector habitats and spillover dynamics. The article further analyzes host–virus and virus–vector interactions, highlighting mechanisms of immune evasion, neurotropism, and vascular disease. Computational and machine learning models are examined as novel instruments for forecasting outbreaks and developing early warning systems. Finally, a summary of present and prospective control options is provided, covering integrated vector management, Wolbachia-based biological control, vaccinations, and antiviral immunotherapies.

Source: 

Link: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2026.1755594/full

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Pre-existing systemic and #nasal #antibodies against avian #H5 #influenza A viruses vary according to #childhood imprinting

 

Abstract

Avian influenza A viruses (IAV) pose a constant pandemic threat, with the recent 2.3.4.4b clade of the H5 subtype causing high pathogenicity and spreading across animal species and geographic locations. Understanding human pre-existing immunity to avian H5 IAV can inform on population susceptibility, a critical aspect of pandemic preparedness. To that end, we analysed the IAV HA-specific antibodies across individuals born between 1928-1999 with different early life exposures to IAV subtypes. Individuals born prior to 1957 had the highest pre-existing serum antibodies to group 1 HA antigens, including the 2.3.4.4b H5 and a group 1 HA stem antigen. These birth-year-specific patterns were not reflected in the limited pre-existing serum neutralising antibodies detectable against a 2.3.4.4b H5 IAV or in H5-specific memory B cell populations. They were however evident in pre-existing nasal IgG and IgA titres to H5, which were greater in individuals born prior to 1957. Our findings demonstrate that the immunological biases afforded by early life exposure extend to antibodies detected in the nasal mucosa, the site of IAV replication.

Source: 

Link: https://www.biorxiv.org/content/10.64898/2026.05.08.723737v1

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G4 #Eurasian avian-like #H1N1 swine #influenza viruses exhibit enhanced #pathogenicity potential in mice and #pigs

 

Abstract

Currently circulating swine influenza viruses (SIVs) mainly include H1N1, H1N2, and H3N2 subtypes. In this study, two G4 genotype Eurasian avian-like (EA) H1N1 SIVs were isolated from 556 samples collected between 2023 and 2026. A systematic analysis was conducted on the two EA H1N1 isolates (FYD30 and YZF69) to assess their pandemic potential. The hemagglutinin (HA) proteins of both H1N1 viruses possessed residues 225E and 228S, indicating enhanced affinity for human-like alpha-2,6-linked sialic acid receptors, which was confirmed by receptor-binding assays. Polymerase activity tests demonstrated that the two SIVs exhibited significantly higher activity in mammalian cells, relative to avian cells, which is consistent with the efficient replication in mammalian cells. Challenge experiments revealed that both H1N1 caused significant pathogenicity in mice and pigs, with YZF69 exhibited higher virulence than FYD30. The higher virulence of YZF69 may be attributed to its molecular features, including the NP Q357K mutation, and an additional glycosylation site in HA. In conclusion, currently circulating EA H1N1 SIVs have acquired key molecular signatures of mammalian adaptation, exhibit enhanced virulence in mammals, and continue to undergo extensive reassortment driven by international swine trade. These findings highlight the potential pandemic risk of SIVs and underscore the urgent need for strengthened surveillance.

Source: 

Link: https://www.biorxiv.org/content/10.64898/2026.05.12.724537v1

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The #Coinfection of #Bartonella spp. and #Hantavirus in Wild #Rodent and #Shrew Species in Eastern #China

 

Abstract

Background:

Bartonella spp. are Gram-negative bacteria that cause diseases including endocarditis, lymphadenopathy, and neuroretinitis. Hantavirus (HV), belonging to the family Hantaviridae, induces illnesses such as hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. Both pathogens exhibit host specificity—defined as a preference or restriction to specific host species or ranges. Rodents and shrews are primary hosts for these pathogens, and their high coinfection rates often indicate elevated risk of human exposure. To our knowledge, however, data on Bartonella spp.–HV coinfection in rodents and shrews from Eastern China remain limited.

Materials and Methods:

Between 2020 and 2023, rodents (n = 311) and shrews (n = 16) were investigated for coinfection with Bartonella spp. and HV in Qingdao, eastern China. Nested Polymerase Chain Reaction (PCR) was used for the detection of RNA-dependent RNA polymerase (RdRp) gene of HV and the Internal Transcribed Spacer, citrate synthase (gltA) and RNA polymerase beta subunit (rpoB) genes of Bartonella spp.

Results:

The overall infection rates of Bartonella spp., HV, and coinfection were 21.4%, 6.7%, and 4.0%, respectively. The highest rates were observed in Apodemus agrarius (53.8%, 21.3%, and 15.0%). Coinfection rates differed significantly by species (p < 0.05), with A. agrarius exhibiting the highest rate (15.0%). Notably, the coinfection rate was significantly higher in male (28.9%) than female A. agrarius (7.1%) (p < 0.05).

Conclusions:

This study confirms the coinfection of Bartonella spp. and HV in rodents in the eastern region of China. Enhanced monitoring of rodent and shrew densities, as well as their carried pathogens, is essential. Additionally, timely screening, diagnosis, and treatment should be conducted for high-risk populations in the region to reduce the incidence of related zoonoses.

Source: 

Link: https://journals.sagepub.com/doi/10.1177/15303667261448824

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#Genomic Analysis of #SinNombre Virus #Sequences, Northwestern #USA, 2023

 

Abstract

We report Sin Nombre virus (SNV) genome sequences in the northwestern United States, including SNV sequences recovered from montane voles. Analysis of samples collected from 189 individual rodents revealed high SNV prevalence in the region and evidence of virus reassortment or coinfection, highlighting ongoing virus diversification in rodents.

Source: 

Link: https://pubmed.ncbi.nlm.nih.gov/42116630/

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Sequencing of #Betacoronavirus erinacei from faeces of pet #hedgehogs demonstrates a continuity of #MERS-CoV like viruses in #European and Eurasian hedgehog species

 

Abstract

Hedgehogs have been recently identified as carriers of Betacoronavirus erinacei (also known as Erinaceus coronavirus, EriCoV) a virus closely related to B. cameli responsible for human Middle East Respiratory Syndrome (MERS), raising questions about the risk of hedgehog-to-human transmission and suggesting the need for coronavirus (CoV) surveillance in hedgehogs. This study investigated the presence of CoVs in fecal samples of hedgehogs kept as pets in Italy in 2021–2022. A pan-CoV nested RT-PCR targeting the RdRp gene was used for screening and positive samples were sequenced and phylogenetically analyzed. Two (6.2%) out of 30 hedgehogs analyzed were positive for B. erinacei represented by 2/3 (66.7%) long eared hedgehog (Hemiechinus auritus) while all the 27 tested African pygmy hedgehog (Atelerix albiventris) were negative. Whole genome sequence obtained from one B. erinacei-positive sample showed closest homology (85.7%) with B. erinacei previously detected in Erinaceus sp. from Eastern Russia. Phylogeny showed that the virus of this study formed a separate clade in the cluster with other B. erinacei identified in Europe and European Russia and did not cluster with other B. erinacei identified in China in Amur hedgehog (E. amurensis). No recombination events were observed. Analysis of the Spike protein revealed the presence of six out of the 11 key receptor binding residues, including two out of the three critical residues recently identified for the binding of Erinaceus europaeus receptor APN and B. erinacei. Results of this study suggest the presence of a long-eared hedgehog-specific strain of B. erinacei. Overall results support the circulation of coronaviruses along a phylogenetic continuum among different species of hedgehogs and geographic locations, suggesting the need for further CoV surveillance in both domestic and wild animals. There is also a need for studies on the affinity of EriCoV with the H. auritus APN specific receptor to confirm its involvement in the viral entry process.

Source: 

Link: https://www.sciencedirect.com/science/article/pii/S2352771426000960?via%3Dihub

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Cross-reactive #human #antibody responses to #H5N1 #influenza virus #neuraminidase are shaped by immune history

 

Abstract

H5N1 highly pathogenic avian influenza viruses have spread globally and pose a pandemic risk. Prior studies suggest that early life exposures to group 1 influenza viruses (H1N1 and H2N2) prime antibodies that cross-react to the hemagglutinin of H5N1, which is also a group 1 virus. However, less is known about how immune history affects antibody responses against the H5N1 neuraminidase (NA). We measured NA inhibition antibodies against multiple H5N1 viruses using sera from 155 individuals born between 1927 and 2016. Individuals likely primed in childhood with H1N1 viruses possessed higher levels of antibodies that cross-react with the NA of H5N1 viruses compared to those primed with H2N2 or H3N2 viruses. While young children rarely possessed cross-reactive N1 antibodies, childhood infections with contemporary H1N1, but not H3N2, viruses elicited them. We also measured antibodies against an H5N5 virus (A6 genotype) that recently caused a fatal infection in the United States. Consistent with the lack of circulation of N5 viruses in humans, we found low levels of antibodies against the N5 NA. Our data suggest that immune history greatly impacts the generation of cross-reactive NA antibodies, and that reassortment with other NAs may increase the risk of H5 infection of humans.

Source: 

Link: https://www.nature.com/articles/s41467-026-72941-4

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The Decline in #Influenza #Antibody Titers and Modifiers of #Vaccine #Immunity from over Ten Years of Serological Data

 

Abstract

Annual influenza vaccination is the cornerstone for seasonal protection, yet antibody responses are highly variable across individuals and over time. To systematically assess the determinants of this heterogeneity, we compiled 20,449 hemagglutination inhibition and neutralization titers from 4,540 participants enrolled in 14 new vaccine studies we conducted and 50 prior studies that collectively span 2010-2023. Seasonal effects dominated, with pre- and post-vaccination titers declining steadily from 2017 onwards, outweighing the influence of age, sex, or repeated vaccination. Titers to B Yamagata remained steady throughout all years examined, suggesting unique durability and offering a reason for lineage extinction. Vaccine timing emerged as a strong and previously underappreciated determinant of immunity, with individuals vaccinated later in the season exhibiting larger post-vaccination titers. Not being vaccinated or receiving the live-attenuated FluMist vaccine in one year significantly enhanced the response to inactivated vaccines in 45% or 68% of cohorts, respectively, whereas antigen dose and adjuvants had modest impact. These findings identify vaccine timing and seasonal context as underrecognized drivers of immunogenicity and provide actionable insights for optimizing influenza vaccination strategies.

Competing Interest Statement

The authors have declared no competing interest.

Funding Statement

This research was supported by the the National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) under the Computational Models of Influenza Immunity (U01 AI187062), LJI & Kyowa Kirin, Inc. (KKNA - Kyowa Kirin North America), and the Bodman family (TE).

Source: 

Link: https://www.medrxiv.org/content/10.64898/2026.01.07.25342310v2

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Computational Structural Analysis Predicts #Host-Range Promiscuity and #Antiviral #Resistance in North #American #H5N1 Lineages

 

Abstract

Influenza A virus has been circulating in birds in Eurasia for more than 146 years, but human infection has been sporadic. H5N1 (clade 2.3.4.4b) has recently infected hundreds of species of wild and domestic birds and mammals in North America. Infections include 71 people in the United States. There have been 2 human fatalities (United States and Mexico). We have integrated time-series analysis, molecular phylogenetics, and structural biology to understand how H5N1 is circulating in North America and adapting to new hosts. Our time-series analysis reveals that the circulation of H5N1 follows a distinct seasonal pattern, with cases in the United States increasing November to April. We also document an increase in the number of cases reported since 2021. We show that H5N1 spreads in North America as 2 distinct lineages. These viral lineages have achieved a vast host range by efficiently binding the viral surface protein hemagglutinin to both mammalian and avian cell surface receptors. This novel host-range promiscuity is concomitant with the strengthening of the viral polymerase basic 2 protein binding for mammalian and avian immune proteins. Once bound, the immune proteins have diminished ability to fight the virus, thus allowing for efficient replication. Our analyses predict that while most antivirals remain effective, a fatal human isolate showed reduced binding to multiple drugs from different classes. The H5N1 virus is causing an animal pandemic through promiscuity of host range and strengthening ability to evade the innate immune systems of both mammalian and avian cells.

Source: 

Link: https://spj.science.org/doi/10.34133/csbj.0066

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Identification and #genetic characterization of a distinct #genotype of #Puumala #orthohantavirus in #Hebei Province, #China

 

Abstract

Orthohantavirus infections pose a significant threat to human health, while numerous orthohantaviruses have been identified, suspected viral infections remain undiagnosed in the world, which highlights the need for further identification and characterization of viruses circulating in humans and host animals. In this study, viral metagenomics was utilized to investigate orthohantaviruses present in tissue samples collected from rodents trapped at the Bashang Grassland of Hebei Province, China. A total of 145 wild rodents belonging to six species were captured in the study area, and 725 tissue samples (lung, liver, kidney, spleen, gut) were collected in 2024. A Puumala orthohantavirus (PUUV), named Guyuan strain, was identified in Myodes rufocanus, with a positive rate of 0.69%. The complete genomic sequences of the L, M, and S segments were obtained and confirmed by Sanger sequencing. Phylogenetic analysis of these genomic sequences with those of other orthohantavirus species showed that the L, M, and S segments clustered with PUUV genomic sequences, while sharing a nucleotide sequence similarity of 81.2%, 80.2%, and 84.3% with previously characterized reference viral strains Kitahiyama128L, Tobetsu_04, and Baltic/205 Cg, respectively. Amino acid homology analysis demonstrated that the sequences exhibited the highest identity to PUUV Hokkaido strain at a level of 95.4%, 94.6%, and 97.0% respectively. Viral particles were observed in lung and kidney tissues using transmission electron microscopy, and viral protein antigen was detected in viral RNA-positive lung, liver, and kidney tissues through immunofluorescence assay with antibodies against the PUUV nucleocapsid protein, thereby confirming the virus’s multiorgan tropism. The results demonstrated that a distinct genotype of PUUV was circulating in rodents in the study areas, which may have implications for zoonotic transmission surveillance and public health management in Hebei Province.

Source: 

Link: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0014250

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When Rare #Zoonoses #Travel: #Andes virus, #Hantavirus Cardiopulmonary Syndrome, and the #Preparedness Gap

 

Abstract

The perspective discusses how the suspected Andes virus (ANDV) associated hantavirus cluster on the expedition cruise ship MV Hondius illustrates a critical preparedness gap in managing rare zoonotic infections in mobile, closed, and medically constrained settings. Focusing on the reported multi-country cluster involving severe hantavirus cardiopulmonary syndrome, deaths, and international passenger dispersal, it highlights the associated diagnostic, clinical, and epidemiological challenges. The perspective also explains why ANDV is distinct among hantaviruses, including its documented potential for limited person-to-person transmission during close and prolonged contact, with insights into the virology and pathogenesis of ANDV. Additionally, it highlights ecological exposure hazards in Patagonia, rodent reservoirs, clinical progression, the necessity for laboratory confirmation, candidate severity biomarkers such as IL-6 and intestinal fatty acid-binding protein, and the lack of specific antiviral therapy. Furthermore, it discusses risk factors, including European ethnicity and host genetic susceptibility linked to αVβ3 integrin variation. Overall, this perspective argues that expedition travel, maritime medicine, One Health surveillance and outbreak preparedness must be better integrated in order to detect, investigate and manage rare but high-consequence zoonotic infections before they escalate into international public health events.

Link: https://pubmed.ncbi.nlm.nih.gov/42107872/

____

A single PA-X #mutation in #bovine-origin #H5N1 #influenza virus reduces #pathogenicity in mice

 

Abstract

Dairy cows have emerged as a reservoir for human infection with highly pathogenic avian influenza (HPAI) H5N1. At the bovine-human interface, H5N1 strains may acquire adaptive mutations that influence their zoonotic potential. Sequence analysis identified a K142E substitution (bovine to human) in the PA and PA-X proteins, with the potential to affect both polymerase activity and host shutoff. Here, we used a loss-of-function approach to investigate how the bovine substitution (E142K) in PA/PA-X impacts viral replication, host shutoff activity, and pathogenicity in the human H5N1 background. Viral growth kinetics demonstrated that the virus containing the E142K substitution is attenuated, with reduced replication compared to wild-type (WT) virus. Consistently, PA-X-mediated host shutoff activity was reduced, resulting in increased induction of interferon (IFN) responses relative to WT. In vivo, mice infected with the E142K mutant virus survived, whereas infection with the WT virus was uniformly lethal. Despite comparable viral titers and inflammation score in mouse lungs, cytokine and chemokine profiling revealed distinct immune responses, with reduced CCL2 and increased CCL5 and IFN-γ in mice infected with the E142K mutant virus compared to mice infected with the WT virus. These findings indicate that increased virulence of the human-adapted strain is driven by a PA-X mutation that modulates inflammatory responses, producing distinct immune signatures linked to host survival or viral lethality rather than changes in polymerase activity by PA. Collectively, these results highlight PA-X as a key determinant of pathogenicity of H5N1 and a potential target for the rational design of antiviral strategies.

Competing Interest Statement

The authors have declared no competing interest.

Source: 

Link: https://www.biorxiv.org/content/10.64898/2026.05.09.724031v1

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#Detection and #isolation of #H5N1 clade 2.3.4.4b #influenza virus from #ticks recovered from a naturally infected slender-billed #gull

 

Abstract

Laridae birds, such as gulls, are known reservoirs of H13 and H16 low pathogenicity avian influenza virus (LPAIV) subtypes. However, during the recent outbreaks linked to the reemergence of high pathogenicity avian influenza virus (HPAIV) H5N1 clade 2.3.4.4b of the Goose/Guangdong lineage, European populations of Laridae birds suffered significant losses. HPAI cases were reported not only along the coastlines but also inland areas, particularly in France and Central Europe. During a diagnostic investigation of a group of Laridae birds, part of a HPAIV outbreak reported in the South of France in 2023, larval stages of Ornithodoros maritimus, a nidicolous soft tick parasitizing seabirds, were recovered from a slender-billed gull (Chroicocephalus genei). Affected birds exhibited gross and histopathological lesions consistent with systemic HPAIV infection. Immunohistochemistry revealed marked neurotropism, oculotropism and multicentric epitheliotropism. Viral isolation and sequencing analysis confirmed the presence of HPAIV H5N1 clade 2.3.4.4b in both the gull and ectoparasites, showing from 99.64% to 100% nucleotide identity across five of eight RNA segments. While additional research is needed to properly assess the vector competence of O. maritimus for HPAIV, ticks may represent an interesting non-invasive surveillance tool for these viruses. This is the first time a HPAIV has been successfully isolated from tick larvae. These findings represent a first step toward understanding the potential role played by ticks in the spread of avian influenza viruses within marine bird colonies and among other ecosystems, considering the occurrence of specific behavioral traits, such as kleptoparasitim and the position of gulls at the interface between wild and domestic species.

Competing Interest Statement

The authors have declared no competing interest.

Funder Information Declared

Agence Nationale de la Recherche, https://ror.org/00rbzpz17

INRAe Animal Health Department

Source: 

Link: https://www.biorxiv.org/content/10.1101/2025.11.28.689408v2

____

#Genetic and #biological characterization of #H9N2 avian #influenza viruses isolated from #swine in #China

 

Abstract

Background

H9N2 avian influenza virus (AIV) has been circulating in poultry in China for decades and are undergoing adaptation to mammals, posing potential pandemic risks. To investigate the prevalence of H9N2 AIVs in swine, we conducted surveillance in Shandong Province from 2021 to 2023.

Results

Two H9N2 influenza virus strains, A/swine/Shandong/417/2021(Sw/SD/417/21) and A/swine/Shandong/662/2022 (Sw/SD/662/22), were successfully isolated from swine and genetically characterized. Phylogenetic analyses showed that both isolates were reassortants containing gene segments from multiple H9N2 AIV lineages and closely related to currently circulating H9N2 AIV. Key molecular marker analysis revealed that both isolates carried mammalian-adaptive residues in the HA receptor-binding sites (183 N, 190 V, 226 L), a novel HA cleavage site variant (PSKSSRGL), PB2 mutations (A588V, E627V), and the M2 S31N substitution, suggesting potential adaptation to mammalian hosts and resistance to adamantane antivirals. Mice infection experiments demonstrated efficient viral replication in the respiratory tract, particularly in the lungs, but only mild histopathological changes were observed, with no significant weight loss or mortality, indicating low pathogenicity in mice. Serological surveillance of 3,172 swine serum samples showed a low prevalence of H9N2 influenza virus infection (0.44%), with positive samples sporadically distributed across regions and years.

Conclusion

In summary, although H9N2 AIV infection in swine is rare and generally mild, the presence of mammalian-adaptive markers and reassortant genomes highlights the potential risk of cross-species transmission and subclinical adaptation. Continuous avian–swine–human influenza surveillance is therefore essential to mitigate the potential threat posed by H9N2 AIV.

Source: 

Link: https://link.springer.com/article/10.1186/s12917-026-05501-z

____

#Coronavirus Disease Research #References (by AMEDEO, May 9 '26)

 

Clin Infect Dis

1. BOZIO CH, Patel M, Reed C, Garg S, et al
   Challenges in defining severe influenza with implications for measuring and communicating influenza vaccine effects.
   Clin Infect Dis. 2026 May 7:ciag304. doi: 10.1093.
   PubMed         Abstract available
   
   

   
   Int J Infect Dis

2. HIBINO M, Shintani A, Murayama H, Morikawa N, et al
   Impact of Early Oral Antiviral Use for Outpatients With COVID-19 on Healthcare Utilization and Recovery (ANCHOR-02).
   Int J Infect Dis. 2026 May 2:108754. doi: 10.1016/j.ijid.2026.108754.
   PubMed         Abstract available
   
   
3. KURITA J, Mukasa K, Kawana Y, Kaneko M, et al
   Cooperative surveillance association and incidence of infectious diseases in Japanese nursery schools.
   Int J Infect Dis. 2026 Apr 30:108757. doi: 10.1016/j.ijid.2026.108757.
   PubMed         Abstract available
   
   
4. LEWIS L, Mohammed D, Evans L, Patterson B, et al
   COVID-19 Hospitalisations in women of childbearing age and children during the Omicron period, January 2023 to September 2024.
   Int J Infect Dis. 2026 Apr 30:108746. doi: 10.1016/j.ijid.2026.108746.
   PubMed         Abstract available
   
   

   
   J Med Virol

5. ANINAGYEI E, Ayivor-Djanie R, Boatey CA, Mateen AA, et al
   High SARS-CoV-2 Antibody Seroprevalence Amid COVID-19 Vaccine Hesitancy Among Unvaccinated Adults in Ghana.
   J Med Virol. 2026;98:e70962.
   PubMed         Abstract available
   
   
6. STONE S, Elsharkawy A, Patterson LD, Natekar JP, et al
   A Novel Humanized Lethal Mouse Model of SARS-CoV-2-Associated Disease.
   J Med Virol. 2026;98:e70959.
   PubMed         Abstract available
   
   

   
   J Virol

7. SUOMALAINEN M, Haenseler W, Kolibius J, Pluckthun A, et al
   Transcriptional transactivation turns human iPSC-derived macrophages into an adenovirus-producing cell state.
   J Virol. 2026 May 4:e0039226. doi: 10.1128/jvi.00392.
   PubMed         Abstract available
   
   
8. ZHANG L, Hoffmann M, Pohlmann S
   Lock out: targeting TMPRSS2 to block influenza and coronaviruses.
   J Virol. 2026 May 4:e0080725. doi: 10.1128/jvi.00807.
   PubMed         Abstract available
   
   

   
   Lancet Infect Dis

9. RITCHIE AJ, Hassan O, Urasa N, Apanga PA, et al
   Safety and immunogenicity of a single-dose adenovirus-vectored rabies vaccine over 1 year in adults and children in Tanzania: interim data from an ongoing, partly randomised, controlled, phase 1b/2 trial.
   Lancet Infect Dis. 2026 Apr 28:S1473-3099(26)00071.
   PubMed         Abstract available
   
   

   
   Nature

10. BAO Z, Liu Z, Zhang Z, Wang X, et al
    Steric hindrance of antibody binding in an Omicron spike fusion intermediate.
    Nature. 2026 May 6. doi: 10.1038/s41586-026-10462.
    PubMed         Abstract available
    
    

    
    Science

11. COHEN J
    Arrest of Fauci aide for records violations decried as political.
    Science. 2026;392:565-566.
    PubMed         Abstract available
    
    
12. COHEN J
    After pulling vaccine study, CDC leader calls for new journal.
    Science. 2026;392:564-565.
    PubMed         Abstract available
    

#Influenza and Other Respiratory Viruses Research #References (by AMEDEO, May 9 '26)

 

Antimicrob Agents Chemother

1. SULEA T, Stuible M, Moreno M, Pelletier A, et al
   Multifunctional ACE2-nanobody fusion design for pan-specific neutralization and cardiovascular protection in SARS coronavirus infection.
   Antimicrob Agents Chemother. 2026;70:e0002426.
   PubMed         Abstract available
   
   

   
   Antiviral Res

2. CHIBA S, Kiso M, Yamayoshi S, Uraki R, et al
   Niclosamide inhibits the replication of highly pathogenic avian influenza A (H5Nx) viruses and antiviral-resistant mutants.
   Antiviral Res. 2026;251:106421.
   PubMed         Abstract available
   
   

   
   Cell

3. HAVENS JL, Kosakovsky Pond SL, Zehr JD, Pekar JE, et al
   Dynamics of natural selection preceding human viral epidemics and pandemics.
   Cell. 2026;189:2762-2775.
   PubMed         Abstract available
   
   

   
   Epidemiol Infect

4. MORTON LC, Forshey BM, Klinkhammer KE, Romaner A, et al
   Local genomic epidemiology investigations of SARS-CoV-2 during the early pandemic response: A global systematic review.
   Epidemiol Infect. 2026;154:e56.
   PubMed         Abstract available
   
   

   
   J Gen Virol

5. SANTOS IA, Grosche VR, Oliveira LDS, Guarda PHS, et al
   Copper(II) benzyloxychalcone analogues as new potential metallodrugs against SARS-CoV-2 replication.
   J Gen Virol. 2026;107:002245.
   PubMed         Abstract available
   
   
6. ISLAM MT, Pillatzki A, Webb B, Ramamoorthy S, et al
   Infectivity of recombinant Torque teno sus virus 1 in pigs coinfected with swine influenza virus.
   J Gen Virol. 2026;107:002264.
   PubMed         Abstract available
   
   
7. SCHAFERS J, Warren CJ, Yang J, Zhang J, et al
   Stability of influenza viruses in the milk of cows and sheep.
   J Gen Virol. 2026;107:002257.
   PubMed         Abstract available
   
   

   
   J Infect

8. BEHARIER O, Guedalia J, Sehtman-Shachar DR, Kerem L, et al
   Maternal SARS-CoV-2 Infection and Early Child Growth and Development: A Nationwide Cohort Study.
   J Infect. 2026 Apr 17:106749. doi: 10.1016/j.jinf.2026.106749.
   PubMed         Abstract available
   
   
9. PEREZ-MAZZALI M, Perez-Cozar F, Cal-Sabater P, Rybakowska P, et al
   Persistent T cell phenotypic alterations and early innate immune dysregulation as potential biomarkers of Long COVID.
   J Infect. 2026 Mar 17:106731. doi: 10.1016/j.jinf.2026.106731.
   PubMed         Abstract available
   
   

   
   J Virol

10. ZHANG L, Hoffmann M, Pohlmann S
    Lock out: targeting TMPRSS2 to block influenza and coronaviruses.
    J Virol. 2026 May 4:e0080725. doi: 10.1128/jvi.00807.
    PubMed         Abstract available
    
    

    
    MMWR Morb Mortal Wkly Rep

11. KIBIGER L, Oltean HN, Leitz L, Krause E, et al
    Fatal Human Case of Highly Pathogenic Avian Influenza A(H5N5) in a Backyard Flock Owner - Washington, November 2025.
    MMWR Morb Mortal Wkly Rep. 2026;75:221-225.
    PubMed         Abstract available
    
    
12. VAUGHAN A, Joyce A, Traub E, Jae M, et al
    Serologic Evidence of Highly Pathogenic Avian Influenza A(H5N1) Virus Infection in a Veterinary Professional Exposed to an Infected Domestic Cat - Los Angeles County, California, December 2024-January 2025.
    MMWR Morb Mortal Wkly Rep. 2026;75:215-220.
    PubMed         Abstract available
    
    

    
    N Engl J Med

13. LITE TL, Goya S, Davis ML, Morris AE, et al
    Human Infection with Highly Pathogenic Avian Influenza A(H5N5) Virus.
    N Engl J Med. 2026 May 7. doi: 10.1056/NEJMc2518178.
    PubMed        
    
    
14. LEROUX-ROELS I, Huang G, Ferguson M, Kohli A, et al
    Efficacy and Safety of an mRNA Seasonal Influenza Vaccine in Adults.
    N Engl J Med. 2026;394:1803-1813.
    PubMed         Abstract available
    
    

    
    PLoS Biol

15. CAMPBELL AJ, Shephard M, Paulos AP, Pauly MD, et al
    Surveillance on California dairy farms reveals multiple possible sources of H5N1 influenza virus transmission.
    PLoS Biol. 2026;24:e3003761.
    PubMed         Abstract available
    
    

    
    PLoS Comput Biol

16. MCTIERNAN J, Zhang Y, Li S, Kuhlman TE, et al
    Clustering of SARS-CoV-2 membrane proteins in lipid bilayer membranes.
    PLoS Comput Biol. 2026;22:e1014229.
    PubMed         Abstract available
    
    

    
    PLoS Med

17. O'HAGAN A, Pechey R, Forde H, Bandy L, et al
    Adherence to voluntary UK sugar, salt, and calorie reduction targets in the highest-grossing restaurant chains: A cross-sectional study.
    PLoS Med. 2026;23:e1004681.
    PubMed         Abstract available
    
    
18. KRUK ME, Kapoor NR, Arsenault C, Carai S, et al
    Health system use and experience among people with poor mental health: A cross-sectional analysis of the People's Voice Survey in 18 countries.
    PLoS Med. 2026;23:e1004745.
    PubMed         Abstract available
    
    

    
    PLoS One

19. DO NASCIMENTO AI, Dos Santos Conrado D, Mareto LK, de Azevedo MV, et al
    Age and vaccine information sources drive vaccine hesitancy: A household survey in Central-Western Brazil.
    PLoS One. 2026;21:e0348412.
    PubMed         Abstract available
    
    
20. SIEBELS K, Ogden NH, Turgeon P, Lapeyre J, et al
    The impact of non-pharmaceutical interventions on the socio-economic and demographic determinants of COVID-19 incidence: A spatial analysis of the pandemic in Toronto, Canada.
    PLoS One. 2026;21:e0347649.
    PubMed         Abstract available
    
    
21. CHU L, Hollar TL, Klimas N, Bertolli J, et al
    Facilitators of and barriers to participation in Long COVID research: A qualitative analysis.
    PLoS One. 2026;21:e0346007.
    PubMed         Abstract available
    
    
22. WEATHERALD J, Wen C, Sharpe H, Leung WM, et al
    Impact of the COVID-19 pandemic on sex-related disparities in cystic fibrosis healthcare utilization and outcomes: A population study.
    PLoS One. 2026;21:e0345009.
    PubMed         Abstract available
    
    
23. BIR C, Jung J, Widmar NO
    Understanding why people value working from home or hybrid workplace flexibility a study of preferences during the COVID-19 pandemic.
    PLoS One. 2026;21:e0348206.
    PubMed         Abstract available
    
    
24. KABATA Y, Terauchi R, Nakano T
    Nationwide trends and regional variation in intravitreal injections and anti-VEGF agent use in Japan from 2017 to 2022: An analysis of the NDB Open Data.
    PLoS One. 2026;21:e0348087.
    PubMed         Abstract available
    
    
25. PARK JH, Lee MT, Jung SY, Choi ST, et al
    Association between coronavirus disease 2019 and new-onset autoimmune diseases during the early phase of the pandemic.
    PLoS One. 2026;21:e0347872.
    PubMed         Abstract available
    
    
26. TORREJON-GUIRADO MC, Baena-Jimenez MA, Lima-Serrano M
    Association between cannabis use and result of COVID-19 testing among scholarised adolescents in Andalusia: A cross-sectional study.
    PLoS One. 2026;21:e0347098.
    PubMed         Abstract available
    
    
27. COSKUNER AKTAS S
    Work-family conflict in times of crisis: The moderating roles of self-efficacy and time-based spousal support during pandemic-induced remote work.
    PLoS One. 2026;21:e0348368.
    PubMed         Abstract available
    
    

    
    Proc Natl Acad Sci U S A

28. WAN Z, Yu L, Yang Z, Luo S, et al
    Stag2-mediated chromatin dynamics regulates antibody class switch recombination.
    Proc Natl Acad Sci U S A. 2026;123:e2536391123.
    PubMed         Abstract available
    
    

    
    Vaccine

29. BEOGO I, Muray M, Kibret TC, Xu Y, et al
    Between racism and rumors: understanding vaccine mistrust among older racially diverse adults in Canada.
    Vaccine. 2026;82:128611.
    PubMed         Abstract available
    
    
30. CONNER TL, Goguet E, Haines-Hull H, Segard A, et al
    mRNA COVID-19 vaccination induces minimal IgA in saliva in the absence of prior clinical or subclinical infection.
    Vaccine. 2026;82:128612.
    PubMed         Abstract available
    
    
31. SARNAIK AY, Khan ZU, Rajeswaran T, Majoe A, et al
    The role of misinformation in COVID-19 vaccine hesitancy in low- & middle-income countries.
    Vaccine. 2026;82:128595.
    PubMed         Abstract available
    
    
32. PAUL KK, Torres M, Shih STF, Gray RT, et al
    Estimating the cost-effectiveness of respiratory syncytial virus (RSV) vaccination program for older Australian adults.
    Vaccine. 2026;82:128591.
    PubMed         Abstract available
    
    
33. GULATI RR, Yaqub F, Goodman AL
    Enhancing uptake of respiratory vaccinations in asthma and chronic obstructive pulmonary disease (COPD) patients: a systematic review.
    Vaccine. 2026;81:128560.
    PubMed         Abstract available
    
    
34. WU JD, Meng H, Guo XL, Liu L, et al
    The immune response of the mRNA vaccine CS-2034 as a heterologous booster before and after breakthrough infection: A follow-up study.
    Vaccine. 2026;81:128575.
    PubMed         Abstract available
    
    
35. HIRAKAWA T, Yamada G, Tokiya M, Furukawa T, et al
    Impact of pre-vaccination active vitamin D levels on COVID-19 mRNA vaccine-induced immunity in a Japanese cohort.
    Vaccine. 2026;81:128576.
    PubMed         Abstract available
    
    
36. KONLAN M, Bhandari R, Owusu SA, Kubio C, et al
    The effect of the COVID-19 pandemic on routine childhood immunization coverage and dropout: A community-based cross-sectional study in the Tamale Metropolis, Ghana.
    Vaccine. 2026;81:128584.
    PubMed         Abstract available
    
    
37. REYNALDI A, Lee WS, Selva KJ, Audsley J, et al
    Immune responses associated with protection from SARS-CoV-2 infection.
    Vaccine. 2026;81:128579.
    PubMed         Abstract available
    
    
38. HIGGINS RA, Mazarakis N, Sun C, Toh ZQ, et al
    Effect of time-of-day vaccination on the antibody response to mRNA and protein COVID-19 vaccine in adults.
    Vaccine. 2026;81:128574.
    PubMed         Abstract available
    
    
39. GASHEMA P, Iradukunda PG, Rudacogora JC, Siddig EE, et al
    Unlocking Africa's vaccine Independence: The critical role of technology transfer and intellectual property.
    Vaccine. 2026;81:128573.
    PubMed         Abstract available
    
    
40. HAVERKATE MR, van de Kassteele J, van den Hof S, Sanders JG, et al
    The effect of pre-booked appointments on COVID-19 vaccine uptake among previously vaccinated older adults during the 2023 autumn campaign in the Netherlands: a regression discontinuity analysis.
    Vaccine. 2026;81:128565.
    PubMed         Abstract available
    
    

    
    Virology

41. ZHU M, Wang R, Li X, Zhou H, et al
    Canine influenza virus at the human-animal interface: origins, adaptive evolution, and implications for public health.
    Virology. 2026;621:110943.
    PubMed         Abstract available
    
    
42. LIU C, Yuan X
    Respiratory viruses as key drivers of pulmonary fibrosis: integrated pathways from barrier injury to immune-fibrotic crosstalk.
    Virology. 2026;620:110913.
    PubMed         Abstract available
    
    
43. SULLIVAN-HILL B, Shahrudin S, Ashley CL, Shields NJ, et al
    SARS-CoV-2 variant-specific enhancement of dendritic heparan sulfate mimetic antiviral activities.
    Virology. 2026;620:110915.
    PubMed         Abstract available
    
    
44. KIMURA R, Hayashi Y, Fujimoto-Sato Y, Ishii H, et al
    Decoding viral evolution through integrative bioinformatics: From genomes to global health.
    Virology. 2026;620:110920.
    PubMed         Abstract available
    
    

    
    Virus Res

45. WANG X, Guo J, Liu J, Jiang J, et al
    Shared genetic structure between COVID-19 and migraine: insights from large-scale genome-wide cross-trait analysis.
    Virus Res. 2026;367:199708.
    PubMed         Abstract available
    
    
46. NAGHIBOSADAT M, Hahn E, Molinaro S, Babuadze G, et al
    Comparison of SARS-CoV-2 variant pathogenicity in a long-COVID Syrian hamster model.
    Virus Res. 2026;367:199711.
    PubMed         Abstract available
    
    
47. SITHOLE MN, Khan MR, Mohammed HA, Khan RA, et al
    A Systematic Review on Vaccine Developmental Approaches: Evaluating Efficacy, and Addressing Challenges of Infectious Diseases in the Post-COVID-19 Era.
    Virus Res. 2026 Apr 1:199720. doi: 10.1016/j.virusres.2026.199720.
    PubMed         Abstract available
    
    
48. LIU Y, Wang C, Yan D, Zhang Y, et al
    Prognostic prediction in COVID-19 patients: a comprehensive analysis of gut microbiota and hematological biomarkers.
    Virus Res. 2026;367:199729.
    PubMed         Abstract available
    

Migratory #bird and marine #mammal #surveillance fails to find #evidence for an HPAI #H5N1 2.3.4.4b incursion into #Australia in 2025

 

Abstract

The panzootic caused by high pathogenicity avian influenza (HPAI) H5N1 clade 2.3.4.4b has been devastating for animals, globally. Despite global spread, the virus remains absent in Oceania. Herein we report the results of our fourth year of enhanced migratory bird surveillance, coinciding with the spring migration of wild birds in 2025; none of the 847 migratory wild birds or 38 marine mammals were positive for HPAI H5N1, although we did detect LPAI. Surveillance remains a critical tool for HPAI H5N1 response, with early detection and rapid response being critical to mitigate the impacts of this virus on animal, environment and human health.

Competing Interest Statement

The authors have declared no competing interest.

Funder Information Declared

Australian Government Department of Agriculture, Fisheries and Forestry

Wildlife Health Australia

Australian Department of Health, Disability and Ageing

Western Australian Marine Science Institution

Department for Environment and Water, https://ror.org/053gv9453

Department of Climate Change, Energy, the Environment and Water of Australia

Department of Climate Change, Energy, the Environment and Water

Source: 

Link: https://www.biorxiv.org/content/10.64898/2026.05.07.722556v1

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Variable #transmission efficiency of #mammalian origin #HPAI D1.1 #H5N1 strains in #ferrets

 

Abstract

Highly pathogenic avian influenza H5N1 2.3.4.4b genotype D1.1 lineage continues to predominate in the United States wild bird population and has spilled over into dairy cattle three independent times. To assess the transmission risk of this sublineage, we performed direct-contact transmission experiments for three distinct D1.1 strains in ferrets. Two of these strains were isolated from humans and one from a lethal cat infection. We found that only one human isolate (A/NV/10/2025) was able to transmit efficiently between ferrets. Compared to the other strains, this isolate harbored the mammalian adaptive PB2 D701N mutation, suggesting this mutation may be critical for D1.1 transmission as opposed to the PB2 E627K substitution present in the lethal cat isolate. Based on these data we conclude that the transmission fitness of D1.1 strains is modest but that special attention should be paid to emergence of adaptation at the PB2 701 position.

Competing Interest Statement

The authors have declared no competing interest.

Funder Information Declared

National Institute of Allergy and Infectious Diseases, https://ror.org/043z4tv69, 75N93021C00015, 75N93021C00017

Source: 

Link: https://www.biorxiv.org/content/10.64898/2026.05.07.722809v1

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Evidence of #Juquitiba and #Jaborá #Orthohantavirus Co-Circulation in #Rodent Communities of #Argentina

 

Abstract

Hantaviruses infect a wide range of vertebrate hosts. In the Americas, Orthohantavirus species pose a major public health concern due to their ability to cause Hantavirus Cardiopulmonary Syndrome (HCPS). Given the lack of approved vaccines and specific treatments for HCPS, identifying circulating orthohantavirus genotypes and their hosts, along with estimating prevalence, is essential for designing effective preventive strategies. The objectives of this study were: (1) to identify the orthohantavirus hosts, (2) to determine the circulating orthohantavirus genotypes, (3) to assess the population structure and dynamics of Akodon montensis, (4) to characterize the rodent communities, and (5) to evaluate associations between A. montensis abundance and environmental variables in two protected natural areas of Misiones Province, Argentina. A total of 12 rodent trapping sessions were conducted from April 2021 to February 2023 in Urugua-í and Cruce Caballero Provincial Parks. Orthohantavirus antibodies were detected in four A. montensis and three Oligoryzomys nigripes from both Provincial Parks. Molecular analyses provided evidence of the co-circulation of Jaborá and Juquitiba orthohantavirus genotypes. Both areas exhibited similar rodent community compositions, with A. montensis as the numerically dominant species and O. nigripes occurring at low abundance. In Urugua-í, A. montensis abundance varied in relation to precipitation and vegetation productivity, whereas no such associations were observed in Cruce Caballero. Further studies are needed to effectively monitor these communities and to gain a better understanding of potential fluctuations in seroprevalence, changes in genotype pathogenicity, and the possible detection of unknown genotypes.

Source: 

Link: https://link.springer.com/article/10.1007/s10393-026-01803-8

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#Fatal #Human Case of #Influenza #H5N5 in a Backyard Flock Owner — #Washington {State}, November 2025

 

Summary

-- What is already known about this topic?

- Since 2022, highly pathogenic avian influenza (HPAI) A(H5) viruses have circulated among wild birds in the United States. Seventy human cases of influenza A(H5), most with mild illness, have been reported in the United States since 2024; 14 human influenza A(H5N1) cases were previously identified in Washington.

-- What is added by this report?

- In November 2025, Washington reported the first human case of HPAI A(H5N5) infection worldwide. A positive laboratory result was obtained from a lower respiratory sample after multiple negative upper respiratory sample results; the patient experienced respiratory failure and died 28 days after symptom onset. The public health investigation identified approximately 135 exposed persons.

-- What are the implications for public health practice?

- Symptom management and testing of exposed persons are critical to monitoring for human-to-human transmission of novel influenza infection. Environmental and animal investigations, including genomic analysis, can identify epidemiologic risk factors.

Abstract

Clade 2.3.4.4b influenza A(H5N1) viruses have circulated across migratory bird flyways in the United States since 2022, including in Washington, where backyard flock detections have been reported annually. In November 2025, a Washington resident died from acute respiratory failure after receiving a positive influenza A(H5) test result at a hospital laboratory. Washington Public Health Laboratories confirmed influenza A(H5), and genomic sequencing identified influenza A(H5N5) virus (A6 genotype). Polymerase chain reaction testing detected highly pathogenic avian influenza A(H5) virus clade 2.3.4.4b from an apparently healthy backyard flock of ducks and sediment from a watering basin on the patient’s property. Six of eight gene segments from the environmental sample and one duck sample (partial neuraminidase segment) were highly genetically similar to the patient’s virus sequence. Although existing wild bird surveillance had not detected influenza A(H5N5) virus (A6) in the U.S. Pacific Flyway, introduction via wild birds into the environment of the backyard flock was likely the source of the patient’s exposure. The public health investigation identified approximately 135 exposed persons; symptom monitoring and influenza testing detected no additional cases. The overall risk for avian influenza A remains low among the general U.S. population; however, novel avian influenza A virus infection should be considered in persons with symptoms of influenza and potential exposures.

Source: 

Link: https://www.cdc.gov/mmwr/volumes/75/wr/mm7517a2.htm?s_cid=OS_mm7517a2_e&ACSTrackingID=USCDC_921-DM155047&ACSTrackingLabel=Week%20in%20MMWR%3A%20Vol.%2075%2C%20May%207%2C%202026&deliveryName=USCDC_921-DM155047

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