Wednesday, August 27, 2025

Comparative single-cell #genomics of two uncultivated #Naegleria species harboring #Legionella #cobionts

 


ABSTRACT

Amoeboflagellates of the genus Naegleria are free-living protists ubiquitously found in soil and freshwater habitats worldwide. They include the “brain-eating amoeba” Naegleria fowleri, an opportunistic pathogen that causes primary amoebic meningoencephalitis, a rare but fatal infection of humans. Beyond their direct pathogenicity, protists can also act as environmental reservoirs for intracellular bacterial pathogens, such as Legionella spp., to persist and multiply in the environment. In this study, we carried out single-cell genome sequencing of two uncultivated Naegleria species isolated from the River Leam in England. From single cells, we generated two highly complete Naegleria genomes. Phylogenetic analyses placed these species as close relatives of Naegleria fultoni and Naegleria pagei. Exploring Naegleria evolutionary genomics, we identified gene families encoding antistasin-like domains, which have been characterized as factors that inhibit coagulation in blood-feeding leeches. Antistasin-like domains were identified in all sequenced Naegleria species and their close relative Willaertia magna, yet are otherwise largely restricted to animal genomes. Significantly, we recovered highly complete bacterial genomes from each Naegleria single-cell sample. Phylogenomic analysis revealed that both bacteria belong to the Legionellaceae family. Both bacterial genomes encode comprehensive sets of secretion systems and effector arsenals. We identified putative Legionella effectors that resemble TAL (Transcription activator-like) effectors from plant pathogenic Xanthomonas spp. in terms of protein sequence and predicted structure, representing a potentially novel class of Legionella effectors. Our study highlights the advantages of single-cell environmental genomics approaches, which enable direct association of intracellular pathogens with their hosts to better understand the evolution of host-pathogen interactions.

Source: mSphere, https://journals.asm.org/doi/full/10.1128/msphere.00352-25?af=R

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An emerging #PB2-627 #polymorphism increases the #zoonotic #risk of avian #influenza virus by overcoming ANP32 host restriction in mammalian and avian hosts

 


ABSTRACT

Alterations in the PB2-627 domain of avian influenza virus (AIV) can potentially increase the risk of cross-host species infections in humans and mammals. Recently, there has been a rise in human cases of AIV infections without the presence of the known mammalian determinant PB2-E627K. Here, we identified a variant, PB2-627V, which has evolved in poultry and has contributed to the increase in human AIV infections. By screening global PB2 sequences, we discovered a new independent cluster of PB2-627V that emerged in the 2010s, prevalent in avian, mammalian, and human AIV isolates, including those of H9N2, H7N9, H3N8, 2.3.4.4b H5N1, and other subtypes. We functionally assessed its host adaptation, fitness, and transmissibility across three subtypes of AIVs (H9N2, H7N9, and H3N8) in different host models. PB2-627V combines the viral properties of avian-like PB2-627E and human-like PB2-627K, facilitating AIVs to efficiently infect and replicate in chickens and mice by utilizing both avian- and human-origin ANP32A proteins. Importantly, PB2-627V promotes efficient transmission between ferrets through respiratory droplets. Deep sequencing of passaged chicken and transmitted ferret viral samples indicates that PB2-627V remains stable across the two host species and shows a high potential for long-term prevalence in avian species. Thus, the PB2-627V mutation in AIVs can stably transmit through poultry and can overcome the cross-species barrier to infect humans. Given the global prominence of AIVs, it will be prudent to monitor influenza viruses for the PB2-627V mutation as a potential marker for zoonotic spread.


IMPORTANCE

Avian influenza viruses (AIVs) are significant zoonotic pathogens. There is a rising trend of human cases of AIVs caused by a range of virus subtypes, including H9N2, H3N8, and H5N1 viruses. Thus, it is crucial to understand the underlying viral changes in AIVs that could result in zoonotic spread. We identify mutation PB2-627V as an emerging viral factor that confers dual ability to the virus to infect and adapt to mammalian and avian hosts, and virus transmissibility in ferrets. The presence of PB2-627V in multiple subtypes of AIVs has the potential to cause public health risk. We therefore propose that PB2-627V be included as a molecular marker to assess the zoonotic risk of AIVs.

Source: Journal of Virology, https://journals.asm.org/doi/full/10.1128/jvi.00853-25?af=R

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#Human-Derived #H3N2 #Influenza A Viruses Detected in #Pigs in Northern #Italy

 


Abstract

In recent years, the four main swine influenza A virus (IAV-S) subtypes circulating in swine in the EU have been H1avN1, H1huN2, H1N1pdm09, and H3N2. The latter emerged in 1984 from a reassortment event between a human seasonal H3N2 and H1avN1, and is currently detected at low prevalence in swine in Italy. Here, we describe nine H3N2 IAV-S isolates belonging to three novel genotypes, first detected in Italy in 2021, likely resulting from reassortment events between swine and human IAVs. The first genotype was characterized by a hemagglutinin (H3 HA) of human seasonal origin, a neuraminidase (N2 NA) derived from H1huN2 strains circulating in Italian swine, and an avian-like internal gene cassette (IGC). The second genotype differed in its IGC constellation: PB2, PB1, PA and NP segments were of pandemic origin (pdm09), while NS and M segments derived from the Eurasian avian-like lineage. The third genotype combined a human-derived H3, a Gent/84-derived N2, and a pdm09-origin IGC, as well as an avian-like NS. This study aimed to characterize the genetic features of these novel H3huN2 and assess their epidemiological relevance, with implications for surveillance and control, improving preparedness and mitigating the risks posed by zoonotic influenza viruses.

Source: Viruses, https://www.mdpi.com/1999-4915/17/9/1171

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#Influenza A Virus #Infection Impairs #Neuronal Activity in Human iPSC-Derived NGN2 Neural Co-Cultures

 


Abstract

Influenza A virus (IAV) infection is associated with a wide variety of neurological complications, of which mild complications like impaired cognitive functioning are most prominent. Even though several studies have shown that many influenza viruses can enter the CNS, the neuropathogenesis of seasonal (H3N2 and H1N1) and pandemic (pH1N1 2009) IAV infections is poorly understood. Therefore, we aimed to investigate the cellular tropism, replication efficiency and associated functional consequences using a human stem cell-derived neural co-culture model of neurons and astrocytes. All viruses were able to infect neurons in the co-culture model, although this infection did not result in efficient replication and release of progeny virus. In addition, infection did not result in visible cell death or apoptosis. However, functional analyses revealed that IAV inoculation resulted in a reduction of spontaneous neural activity and a partial reduction of neural excitability. This study shows that seasonal and pandemic IAVs can disrupt neural homeostasis, without efficient virus replication or the induction of cell death. However, these functional changes in neural activity can contribute to cognitive problems during IAV infections in the acute and potentially post-acute phase of the infection.


Competing Interest Statement

The authors have declared no competing interest.


Funder Information Declared

ZonMw, The Dutch Organisation for knowledge and innovation in health, healthcare and well-being, https://ror.org/01yaj9a77, 91718308

The netherlands organisation for scientific research, OCENW.XS22.2.045, 024.003.001

Escmid, xx

European Union, 101084171

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.08.26.672266v1

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Tuesday, August 26, 2025

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    Variation of COVID-19 vaccination percentage by industry and occupation in Michigan.
    Vaccine. 2025;61:127349.
    PubMed         Abstract available

  44. ALSHURMAN BA, Majowicz SE, Grindrod K, Goh J, et al
    Psychometric validation of the COVID-19 vaccine hesitancy scale for primary and booster doses among university students: A cross-sectional study.
    Vaccine. 2025;61:127368.
    PubMed         Abstract available

  45. OMAR M, Shibli H, Edelstein M
    Comparative impact of the COVID-19 pandemic on parental behaviour towards childhood vaccination in Israel and the United Kingdom: A self-controlled matched cross-sectional study.
    Vaccine. 2025;61:127385.
    PubMed         Abstract available

  46. MARIJAM A, Marijic P, Puggina A, Cailloux O, et al
    Older adults' and physicians' preferences for respiratory syncytial virus vaccination in Germany and Italy: A discrete choice experiment.
    Vaccine. 2025;61:127390.
    PubMed         Abstract available

  47. ROSENTHAL S, Chuah ASF, Kim HK, Ho SS, et al
    Direct and indirect experiences, risk perceptions, and vaccine booster intention: A mediation study in Singapore using secondary risk theory.
    Vaccine. 2025;61:127435.
    PubMed         Abstract available

  48. AL-KASSAB-CORDOVA A, Mezones-Holguin E, Kaufman JS
    Education as a mediator of ethnic disparities in adult COVID-19 vaccination in Peru.
    Vaccine. 2025;61:127436.
    PubMed         Abstract available

  49. MACKE CA, Carreon SA, Desai KR, Minard CG, et al
    COVID-19 vaccine uptake and attitudes in emerging adults with type 1 diabetes.
    Vaccine. 2025 Apr 6:127083. doi: 10.1016/j.vaccine.2025.127083.
    PubMed         Abstract available

  50. LLOYD PC, Acharya G, Zhao H, Chen B, et al
    Safety monitoring of health outcomes following influenza vaccination during the 2023-2024 season among U.S. Commercially-insured individuals aged 6 months through 64 years: Self-controlled case series analyses.
    Vaccine. 2025;63:127614.
    PubMed         Abstract available


    Virology

  51. BADR C, Arbi M, Souiai O, Larbi I, et al
    Tracing the 2021 equine influenza Outbreak: First characterization and phylogeographic analysis of H3N8 Florida clade 1 virus in Tunisia.
    Virology. 2025;611:110655.
    PubMed         Abstract available


    Virus Res

  52. MARTINEZ ES, Fuchs S, Szurmant H, Chen X, et al
    COVID-19 mRNA vaccine immune response to the addition of osteopathic manipulative treatment with lymphatic pumps: a randomized controlled trial.
    Virus Res. 2025 Jul 14:199607. doi: 10.1016/j.virusres.2025.199607.
    PubMed         Abstract available

  53. WU J, Qian X, Bai S, Wu L, et al
    Lesser-known non-apoptotic programmed cell death in viral infections.
    Virus Res. 2025;359:199612.
    PubMed         Abstract available

#Sources and sinks of #influenza A virus genomic diversity in #swine from 2009 to 2022 in the #US

 


ABSTRACT

Influenza A virus (IAV) in swine in the U.S. is surveilled to monitor genetic evolution to inform intervention efforts and aid pandemic preparedness. We describe data from the U.S. Department of Agriculture National Surveillance Plan for Influenza A Virus in Pigs from 2009 to 2022. Clinical respiratory cases were subtyped, followed by sequencing of hemagglutinin (HA) and neuraminidase (NA), and a subset of viruses was whole genome sequenced. Phylogenetic analysis identified geographic and temporal IAV reassortment hotspots. Regions acting as IAV genomic diversity sources or sinks were quantified, and dissemination was qualified and modeled. The dominant IAV clades were H1N2 (1B.2.1), H3N2 (1990.4.a), and H1N1 (H1-1A.3.3.3-c3). Internal genes were classified as triple-reassortant (T) or pandemic 2009 (P), and three genome constellations represented 73.5% of detections across the last 2 years. In some years, the distribution of IAV diversity was so narrow that it presented a statistical signal associated with local adaptation. We also demonstrated that the source of most IAV genomic diversity was in Midwest states (IL, MO, IA), and while this was correlated with swine inventory, the emergence and persistence of diversity were tied to swine transport across the U.S. The continued regional detection of unique HA, NA, and genome constellations provides support for targeted interventions to improve animal health and enhance pandemic preparedness.


IMPORTANCE

Variation in the genetic diversity of influenza A virus (IAV) in swine through time and between regions impacts control efforts. This study quantified the genomic diversity of swine IAV collected from 2009 to 2022 at regional and national levels and modeled sources and sinks of that diversity. Seasonal patterns of IAV transmission were observed, and some locations contributed disproportionately to the emergence of genomic diversity. Minor groups of viruses had the potential to disseminate across the U.S. with animal movement. The identification of these patterns demonstrates the importance of a robust surveillance system to inform vaccine updates that reflect regional patterns of genetic diversity. We show how preemptive interventions in swine IAV diversity hubs could reduce reassortment and the emergence of novel genomic diversity, and how these efforts are likely to reduce the transmission of IAV within swine and between swine and humans.

Source: Journal of Virology, https://journals.asm.org/doi/full/10.1128/jvi.00541-25?af=R

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#Bulgaria - High pathogenicity avian #influenza #H5N1 viruses (#poultry) (Inf. with) - Immediate notification

 


Poultry farms in Plovdiv Region.

Source: WOAH, https://wahis.woah.org/#/in-review/6746

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Serological #Evidence of #Flavivirus #Infection Among #Mothers of #Newborns in El Paso, #Texas

 


Abstract

Background

Flaviviruses represent a significant worldwide threat to human health and have the potential to emerge and cause outbreaks in non-endemic geographical regions. Ongoing surveillance for these viruses in the United States–Mexican border communities such as El Paso, Texas, is lacking. As a continuing effort to better understand the prevalence and to determine which arboviruses are endemic, the aim of this study was to determine the prevalence rate of specific flavivirus antibody among 910 human umbilical cord blood samples obtained from mothers who delivered newborns in El Paso, Texas.

Materials and Methods

The samples were screened for West Nile virus (WNV) and dengue virus (DENV) IgG antibodies with an enzyme-linked immunosorbent assay and confirmed by a plaque reduction neutralization test for DENV, WNV, Zika virus (ZIKV) and Saint Louis encephalitis virus (SLEV).

Results

Among the 910 samples, 2% were positive for specific IgG antibody to DENV, 4.4% to WNV, 0.1% to SLEV, and 0.0% for ZIKV antibody. The results confirmed the local transmission of WNV and supported a low prevalence rate for DENV, and this was the first reported serological evidence of SLEV infection in the El Paso community.

Conclusion

The interpretation of the public health significance of these observations supported previous findings of ongoing transmission of WNV and suggested the possibility of DENV transmission and re-emergence of SLEV in the community. Therefore, prospective studies are needed to obtain a more conclusive understanding of the prevalence of flaviviruses in the El Paso community.

Source: Vector-Borne and Zoonotic Diseases, https://www.liebertpub.com/doi/abs/10.1177/15303667251367518

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Single-Cell #Network #Analysis Identifies CLEC4E as a Key Mediator of Proinflammatory mDC Responses in #Influenza #Infection

 


Abstract

The severity of influenza is often driven by an excessive host immune response rather than the virus itself, yet the key molecular drivers within specific immune cells remain poorly understood. While recent single-cell RNA sequencing studies have successfully identified immune populations involved, they have largely not identified the upstream drivers modulating their pro-inflammatory functions. Here we employed an integrated single-cell co-expression network to address this gap. Our analysis identified myeloid dendritic cells (mDCs) as central to pro-inflammatory response during infection. Through a multi-layered key driver analysis, we pinpointed C-type lectin, CLEC4E as a top candidate modulating this pathological inflammatory response. The role of CLEC4E was confirmed in an independent single-cell dataset from influenza-infected patients and further validated in vivo. Pharmacological inhibition of CLEC4E in a murine influenza model significantly reduced disease severity and lower viral titers in the lungs. This study not only clarifies that CLEC4E overexpression in mDCs contributes to pro-inflammatory signaling pathways influencing influenza severity but also shows the power of single-cell network approaches to uncover novel and robust therapeutic targets hidden within complex immune responses.


Competing Interest Statement

The M.S. laboratory has received unrelated funding support in sponsored research agreements from Phio Pharmaceuticals, 7Hills Pharma, ArgenX NV, Ziphius and Moderna.

Funder Information Declared

NIH Common Fund, R21AI149013, R01AI170112, U01AG088351

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.08.21.671587v1

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Monday, August 25, 2025

History of Mass Transportation: The FS Ale 801 Electric Multiple Unit

 


Di Christof Hofbauer - http://www.bahnbilder.ch/picture/8345, GFDL, https://commons.wikimedia.org/w/index.php?curid=16838742

Source: Wikipedia, https://it.wikipedia.org/wiki/Automotrici_FS_ALe_801_e_ALe_940

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Sunday, August 24, 2025

The Great Day of His Wrath, John Martin (1851 - 1853)

 


Public Domain.

Source: WikiArt, https://www.wikiart.org/en/john-martin/the-great-day-of-his-wrath-1853

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Saturday, August 23, 2025

#SARS-CoV-2 #antibody responses in #children exhibit higher FcR engagement and avidity than in adults

 


Abstract

As intrinsic differences in humoral immune response to SARS-CoV-2 between children and adults remain unclear, we improved characterisation by defining the kinetics, specificity and function of antibodies to SARS-CoV-2 in children (n = 146, aged 9.4 ± 4.8 years with n = 257 samples) compared to adults (n = 85, aged 39.5 ± 15.2 years with n = 122 samples). We used plasma samples from an infection and vaccination-naive cohort study with RT-PCR confirmed ancestral B.1* SARS-CoV-2 virus infection with asymptomatic or mild disease, collected in Hong Kong between March to December 2020, from acute (0–14 days post infection) to convalescent (15–206 days) timepoints. Children had significantly lower primary antibody responses against SARS-CoV-2 proteins overall, leading to a less isotype switched response. While children had lower OC43 Spike and SARS-CoV-2 S2 IgG and avidity than adults, they exhibited higher avidities for SARS-CoV-2 whole Spike and Nucleocapsid, and higher levels of Spike FcγR-binding antibodies. Adults’ SARS-CoV-2 antibody responses could be derived from high avidity pre-existing cross-reactive common cold coronavirus B cell responses, whilst children appear to generate a de novo SARS-CoV-2- specific Spike and Nucleocapsid IgG with robust Fc receptor (FcR) binding ability and high avidity at a higher proportion than adults, thus their responses are more targeted and functional for SARS-CoV-2.

Source: Nature Communications, https://www.nature.com/articles/s41467-025-63263-y

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History of Mass Transportation: The DB V 200 Hydraulic Diesel Locomotive

 


By Matthew Black - originally posted to Flickr as DPP_0612, CC BY-SA 2.0, https://commons.wikimedia.org/w/index.php?curid=7171062

Source: Wikipedia, https://en.wikipedia.org/wiki/DB_Class_V_200

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#SARS-CoV-2 #infection induces pro-fibrotic and pro-thrombotic #foam cell #formation

 


Abstract

COVID-19 and long COVID are characterized by a dysregulated immune response. However, the role of macrophages during viral infection is poorly defined. Here we demonstrate that SARS-CoV-2 infection results in increased macrophage numbers and extensive formation of enlarged lipid-laden macrophages or foam cells using humanized mice, rhesus macaques and post-mortem human lung tissue. Notably, infection by other coronaviruses tested, SARS-CoV-1, MERS-CoV and two bat coronaviruses (SHC014-CoV or WIV1-CoV), did not result in macrophage proliferation or foam cell formation. Foam cells in SARS-CoV-2-infected human lung tissue display a pro-fibrotic and pro-thrombotic phenotype as they are enriched for genes associated with platelet activation and aggregation, as well as extracellular matrix organization and collagen synthesis. After viral clearance, macrophage numbers remain elevated, and lung fibrosis and thrombi persist. Importantly, we show that pre-exposure prophylaxis or early treatment with a SARS-CoV-2 antiviral, EIDD-2801, prevents increases in macrophage cell numbers and foam cell formation, and reduces fibrosis markers. These observations highlight the contribution of macrophages to lung inflammation and tissue injury leading to the pulmonary fibrosis observed in COVID-19 patients.

Source: Nature Microbiology, https://www.nature.com/articles/s41564-025-02090-9

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Friday, August 22, 2025

#Famine confirmed for first time in #Gaza (#WHO, August 22 '25)

 


More than half a million people in Gaza are trapped in famine, marked by widespread starvation, destitution and preventable deaths, according to a new Integrated Food Security Phase Classification (IPC) analysis released today

Famine conditions are projected to spread from Gaza Governorate to Deir Al Balah and Khan Younis Governorates in the coming weeks.

The Food and Agriculture Organization of the United Nations (FAO), UNICEF, the United Nations World Food Programme (WFP) and the World Health Organization (WHO) have collectively and consistently highlighted the extreme urgency for an immediate and full-scale humanitarian response given the escalating hunger-related deaths, rapidly worsening levels of acute malnutrition and plummeting levels of food consumption, with hundreds of thousands of people going days without anything to eat.

The agencies reinforced that famine must be stopped at all costs. An immediate ceasefire and end to the conflict is critical to allow unimpeded, large-scale humanitarian response that can save lives. 

The agencies are also gravely concerned about the threat of an intensified military offensive in Gaza City and any escalation in the conflict, as it would have further devastating consequences for civilians where famine conditions already exist. 

Many people – especially sick and malnourished children, older people and people with disabilities – may be unable to evacuate.

By the end of September, more than 640 000 people will face Catastrophic levels of food insecurity – classified as IPC Phase 5 – across the Gaza Strip. 

An additional 1.14 million people in the territory will be in Emergency (IPC Phase 4) and a further 396 000 people in Crisis (IPC Phase 3) conditions. 

Conditions in North Gaza are estimated to be as severe – or worse – than in Gaza City. However, limited data prevented an IPC classification, highlighting the urgent need for access to assess and assist. Rafah was not analyzed given indications that it is largely depopulated.

Classifying famine means that the most extreme category is triggered when three critical thresholds – extreme food deprivation, acute malnutrition and starvation-related deaths – have been breached. The latest analysis now affirms on the basis of reasonable evidence that these criteria have been met.

Almost two years of conflict, repeated displacement, and severe restrictions on humanitarian access, compounded by repeated interruptions and impediments to access to food, water, medical aid, support to agriculture, livestock and fisheries and the collapse of health, sanitation, and market systems, have pushed people into starvation.

Access to food in Gaza remains severely constrained. In July, the number of households reporting very severe hunger doubled across the territory compared to May and more than tripled in Gaza City. More than one in three people (39 percent) indicated they were going days at a time without eating, and adults regularly skip meals to feed their children.

Malnutrition among children in Gaza is accelerating at a catastrophic pace. In July alone, more than 12 000 children were identified as acutely malnourished – the highest monthly figure ever recorded and a six-fold increase since the start of the year. Nearly one in four of these children were suffering from severe acute malnutrition (SAM), the deadliest form with both short and long-term impacts.

Since the last IPC Analysis in May, the number of children expected to be at severe risk of death from malnutrition by the end of June 2026 has tripled from 14 100 to 43 400. Similarly, for pregnant and breastfeeding women, the number of estimated cases has tripled from 17 000 in May to 55 000 women expected to be suffering from perilous levels of malnutrition by mid-2026. The impact is visible: one in five babies are born prematurely or underweight.

The new assessment reports the most severe deterioration since the IPC began analyzing acute food insecurity and acute malnutrition in the Gaza Strip, and it marks the first time a famine has been officially confirmed in the Middle East region.

Since July, food and aid supplies entering Gaza increased slightly but remained vastly insufficient, inconsistent and inaccessible compared to the need.

Meanwhile, approximately 98 percent of cropland in the territory is damaged or inaccessible – decimating the agriculture sector and local food production – and nine of ten people have been serially displaced from homes. Cash is critically scarce, aid operations remain severely disrupted, with most UN trucks looted amid growing desperation. Food prices are extremely high and there are not enough fuel and water to cook and medicines and medical supplies.

Gaza’s health system has severely deteriorated, access to safe drinking water and sanitation services has been drastically reduced, while multi-drug resistant infections are surging and levels of morbidity – including diarrhoea, fever, acute respiratory and skin infections – are alarmingly high among children.

To enable lifesaving humanitarian operations, the U.N. agencies emphasized the importance of an immediate and sustained ceasefire to stop the killing, allow for the safe release of hostages and permit unimpeded access for a mass influx of assistance to reach people across Gaza. 

They stressed the urgent need for greater amounts of food aid, along with dramatically improved delivery, distribution and accessibility, as well as shelter, fuel, cooking gas and food production inputs. 

They emphasized that it is critical to support the rehabilitation of the health system, maintain and revive essential health services, including primary health care, and ensure sustained delivery of health supplies into and across Gaza. The restoration of commercial flows at scale, market systems, essential services, and local food production is also vital if the worst outcomes of the famine are to be avoided.

“People in Gaza have exhausted every possible means of survival. Hunger and malnutrition are claiming lives every day, and the destruction of cropland, livestock, greenhouses, fishery and food production systems has made the situation even more dire,” said FAO Director-General QU Dongyu. “Our priority must now be safe and sustained access for large-scale food assistance. Access to food is not a privilege – it is a basic human right.”

“Famine warnings have been clear for months,” said Cindy McCain, WFP Executive Director. “What’s urgently needed now is a surge of aid, safer conditions, and proven distribution systems to reach those most in need – wherever they are. Full humanitarian access and a ceasefire now are critical to save lives.”

“Famine is now a grim reality for children in Gaza Governorate, and a looming threat in Deir al-Balah and Khan Younis,” said UNICEF Executive Director Catherine Russell. “As we have repeatedly warned, the signs were unmistakable: children with wasted bodies, too weak to cry or eat; babies dying from hunger and preventable disease; parents arriving at clinics with nothing left to feed their children. There is no time to lose. Without an immediate ceasefire and full humanitarian access, famine will spread, and more children will die. Children on the brink of starvation need the special therapeutic feeding that UNICEF provides.”

“A ceasefire is an absolute and moral imperative now,” said WHO Director-General Dr Tedros Adhanom Ghebreyesus. “The world has waited too long, watching tragic and unnecessary deaths mount from this man-made famine. Widespread malnutrition means that even common and usually mild diseases like diarrhoea are becoming fatal, especially for children. The health system, run by hungry and exhausted health workers, cannot cope. Gaza must be urgently supplied with food and medicines to save lives and begin the process of reversing malnutrition. Hospitals must be protected so that they can continue treating patients. Aid blockages must end, and peace must be restored, so that healing can begin.”

 

Notes for editors

Access the IPC alert https://www.ipcinfo.org/fileadmin/user_upload/ipcinfo/docs/IPC_Famine_Review_Committee_Report_Gaza_Aug2025.pdf.

The Integrated Food Security Phase Classification (IPC) is an innovative 21-partner initiative – made up of UN agencies and international NGOs – for improving food security and nutrition analysis and decision-making. By using the IPC classification and analytical approach, governments, UN Agencies, NGOs, civil society and other relevant actors, work together to determine the severity and magnitude of acute and chronic food insecurity, and acute malnutrition situations in a country, according to internationally-recognized scientific standards. Find out more https://www.ipcinfo.org/ipcinfo-website/ipc-overview-and-classification-system/en/.

Source: World Health Organization, https://www.who.int/news/item/22-08-2025-famine-confirmed-for-first-time-in-gaza

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#USA, #Wastewater Data for Avian #Influenza #H5 (#CDC, August 22 '25)

 


{Summary}

Time Period: August 10, 2025 - August 16, 2025

-- H5 Detection1 site (0.2%)

-- No Detection422 sites (99.8%)

-- No samples in last week37 sites




(...)

Source: US Centers for Disease Control and Prevention, https://www.cdc.gov/nwss/rv/wwd-h5.html

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History of Mass Transportation: The Mekarski Tram System

 


By Gonioul - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=3584482

Source: Wikipedia, https://en.wikipedia.org/wiki/Mekarski_system

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Thursday, August 21, 2025

#Argentina - High pathogenicity avian #influenza #H5 viruses (#poultry) (Inf. with) - Immediate notification


The event occurred in a laying hen farm in the province of Buenos Aires. The birds involved showed mortality and clinical signs consistent with high pathogenicity avian influenza. The suspicion is officially addressed and samples were taken. The samples tested positive for HPAI H5.

The event occurred in a laying hen farm. On 17/08/25 National Service of Agri-Food Health and Quality (SENASA) received a notification concerning mortality and clinical signs consistent with high pathogenicity avian influenza (HPAI) (drooping and diarrhea). The suspicion is officially addressed, with restriction of the establishment and taking of samples for diagnosis. On 19/08/2025, samples tested positive for HPAI H5. The stamping out of all the birds in the establishment will be carried out. We will update the population data in the following follow-up reports.

Source: WOAH, https://wahis.woah.org/#/in-review/6737

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#Italy, Integrated #Surveillance for #WNV and #USUV - Weekly #Bulletin no. 6 - August 21 2025 (summary): 76 new cases, total so far 351



{Summary}

-- During current surveillance week (14 to 20 August), seventy-six new human confirmed cases of West Nile Virus infection have been reported;

-- So far this year, 351 human cases of WNV infection were confirmed (they were 275 last week); of these:

- 158 were WNND (West Nile Neuroinvasive Disease): 6 in Piedmont, 8 Lombardy, 10 Veneto, 1 Friuli-Venezia Giulia, 8 Emilia-Romagna, 59 Latium, 54 Campania, 2 Basilicata, 5 Calabria, 5 Sardinia;

- 27 were asymptomatic cases in blood donors, 

- 162 were West Nile Fever cases, 

- 2 asymptomatic and 

- 2 symptomatic (unspecified). 

-- Among confirmed cases, there were 22 fatalities: 1 in Piedmont, 1 Lombardy, 10 Latium, 9 Campania, 1 Calabria). 

- The Case-Fatality Rate in WNND cases is thus far at 13.9% (in 2018 it was 20%, and in 2024, 14%). 

-- No confirmed cases of Usutu Virus infection have been confirmed this week.

(...)

Source: High Institute of Health, https://www.epicentro.iss.it/westnile/bollettino/Bollettino_WND_2025_06.pdf

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