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Showing posts from December 28, 2024

Molecular #Evolution of the #H5 and #H7 Highly Pathogenic Avian #Influenza Virus #Haemagglutinin Cleavage Site Motif

ABSTRACT Avian influenza viruses are ubiquitous in the Anatinae subfamily of aquatic birds and occasionally spill over to poultry . Infection with low pathogenicity avian influenza viruses generally leads to subclinical or mild clinical disease. In contrast, highly pathogenic avian influenza viruses emerge from low pathogenic forms and can cause severe disease associated with extraordinarily high mortality rates. Here, we describe the natural history of avian influenza virus , with a focus on H5Nx and H7Nx subtypes , and the emergence of highly pathogenic forms; we review the biology of AIV; we examine cleavage of haemagglutinin by host cell enzymes with a particular emphasis on the biochemical properties of the proprotein convertases, and trypsin and trypsin-like proteases ; we describe mechanisms implicated in the functional evolution of the haemagglutinin cleavage site motif that leads to emergence of HPAIVs; and finally, we discuss the diversity of H5 and H7 haemagglutinin cleavage...

#Coronavirus Disease Research #References (by AMEDEO, December 28 2024)

  Ann Intern Med Annals Video Summary - Anticoagulation Among Patients Hospitalized for COVID-19. Ann Intern Med. 2024 Dec 24:e2403257VS. doi: 10.7326/ANNALS-24-03257. PubMed          VALE CL, Godolphin PJ, Fisher DJ, Higgins JPT, et al Anticoagulation Among Patients Hospitalized for COVID-19 : A Systematic Review and Prospective Meta-analysis. Ann Intern Med. 2024 Dec 24. doi: 10.7326/ANNALS-24-00800. PubMed           Abstract available SHAPPELL CN, Anesi GL Anticoagulation for COVID-19: Seeking Clarity and Finding Yet More Gray. Ann Intern Med. 2024 Dec 24. doi: 10.7326/ANNALS-24-03244. PubMed          Clin Chem FALAK S, O'Sullivan DM, Cleveland MH, Cowen S, et al The Application of Digital PCR as a Reference Measurement Procedure to Support the Accuracy of Quality Assurance for Infectious Disease Molecular Diagnostic Testing. Clin Chem. 2024 Dec 26:hvae187...

#Influenza and Other Respiratory Viruses Research #References (by AMEDEO, Dec. 28 ' 24)

  Antiviral Res WANG B, Xia H, Peng BH, Choi EJ, et al Pellino-1, a therapeutic target for control of SARS-CoV-2 infection and disease severity. Antiviral Res. 2024;233:106059. PubMed           Abstract available SCHRELL L, Fuchs HL, Dickmanns A, Scheibner D, et al Inhibitors of dihydroorotate dehydrogenase synergize with the broad antiviral activity of 4'-fluorouridine. Antiviral Res. 2024 Dec 3:106046. doi: 10.1016/j.antiviral.2024.106046. PubMed           Abstract available Epidemiol Infect SUTER J, Devos I, Matthes KL, Staub K, et al The health and demographic impacts of the "Russian flu" pandemic in Switzerland in 1889/1890 and in the years thereafter. Epidemiol Infect. 2024;152:e174. PubMed           Abstract available JAMA ANDERER S CDC Calls Attention to Underuse of Antiviral Treatment in Higher-Risk Youth With Influenza. JAMA. 2024 Dec 27. ...

Virological and #antigenic characteristics of #SARS-CoV-2 #variants #LF721, #NP1, and #LP81

Abstract XEC and KP.3.1.1 have surpassed KP.3 to become the globally dominant lineages due to their unique NTD mutations. However, several emerging JN.1 sublineages , such as LF.7.2.1, MC.10.1, NP.1 , and, especially, LP.8.1, have demonstrated superior growth advantages compared to XEC . It is critical to access the virological and antigenic characteristics of these emerging SARS-CoV-2 variants. Here, we found that LF.7.2.1 is significantly more immune invasive than XEC, primarily due to the A475V mutation, which enabled the evasion of Class 1 neutralizing antibodies . However, LF.7.2.1's weak ACE2 binding affinity substantially impaired its fitness . Likewise, MC.10.1 and NP.1 exhibited strong antibody immune evasion due to the A435S mutation, but their limited ACE2 engagement efficiency restricted their growth advantage , suggesting that A435S may regulate the Spike conformation, similar to the NTD glycosylation mutations found in KP.3.1.1 and XEC . Most importantly, we found tha...