ETIDIOH - NEW

Highlights •  The present analysis addresses the paucity of genetic information available for the recently emerged KFDV strains. •  As the virus is classified as a highly dangerous pathogen, it is essential to expand the existing genetic information. •  Continuous surveillance of the virus is essential for the development of a vaccine. •  The present study presents new findings on the KFD virus strains that were introduced into circulation in the period 2018-2020. •  The nanopore sequencing technology is presented as a proof of concept for the provision of early warnings in the field. Abstract The future of infectious agent detection and molecular characterization lies in field-forward, on-site strategies . The lack of genomic information for recently circulating Kyasanur Forest Disease virus strains is critical. Kyasanur Forest Virus Disease virus PCR-positive samples from 2018 to 2020 were selected for sequencing. Detailed molecular phylogenetic analyses were ...

Abstract Influenza A viruses (IAV) are primarily transmitted between mammals by the respiratory route , and encounter bacteria in the respiratory tract before infecting susceptible epithelial cells. Previous studies have shown that mammalian-origin IAV can bind to the surface of different bacterial species and purified bacterial lipopolysaccharides (LPS), but despite the broad host range of IAV, few studies have included avian-origin IAV in these assessments. Since IAV that circulate in humans and birds are well-adapted to replication in the human respiratory and avian gastrointestinal tracts , respectively, we investigated the ability of multiple human and avian A(H1N1) IAV to associate with bacteria and their surface components isolated from both host niches. Binding interactions were assessed with microbial glycan microarrays, revealing that seasonal and avian IAV strains exhibited binding diversity to multiple bacterial glycans at the level of the virus and the bacterium, independe...