Skip to main content

Immunogenicity and protective #efficacy of an #intranasal NA-based #influenza virus #vaccine adjuvanted ...

Image
By G.M.

Abstract

Influenza virus neuraminidase (NA) has emerged as a promising vaccine candidate due to its relatively stable antigenic structure and the ability of NA-specific antibodies to provide cross-protection within influenza virus subtypes. Since the influenza virus causes respiratory infections in humans, developing mucosal vaccines to protect the entry site of the virus is of high importance. Recombinant NA requires adjuvants to induce a protective immune response after mucosal administration. In the current study, we analyze the immunogenicity and protective efficacy of a recombinant NA-based influenza virus vaccine administered intranasally in combination with adjuvants consisting of outer membrane proteins from Neisseria meningitidis complexed with exogenous lipopolysaccharides (LPS) from Shigella flexneri or endogenous LPS from N. meningitidis. We evaluated the local and systemic humoral and cellular immune responses to adjuvanted recombinant N1 NA, analyzing the dynamics of local follicular T-helper (Tfh) cells and germinal center B cells (GCB) in nasal-associated lymphoid tissue (NALT) and tissue-resident memory T cells in lungs, as well as the levels of IgA and IgG in the upper and lower respiratory tracts. Finally, we performed a heterologous challenge study to test the ability of the investigated vaccine formulations to induce cross-protection. The study demonstrates that bacterial cell membrane-derived adjuvants significantly improve the immunogenicity and protective efficacy of the recombinant N1 NA-based influenza vaccine leading to protection against clade 2.3.4.4b H5N1 challenge. This finding supports the potential of these adjuvanted vaccines in providing effective mucosal immunity against influenza virus.

Source: BioRxIV, https://www.biorxiv.org/content/10.1101/2025.02.26.640278v1

____

Labels:

Comments

Post a Comment

Popular posts from this blog

#USA, APHIS Confirms {Avian #Influenza #H5N1} #D11 #Genotype in Dairy #Cattle in #Nevada

By G.M.
On January 31, 2025, the USDA Animal and Plant Health Inspection Service (APHIS) National Veterinary Services Laboratories (NVSL) confirmed by whole genome sequence the first detection of highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.4b, genotype D1.1 in dairy cattle .  This confirmation was a result of State tracing and investigation, following an initial detection on silo testing under the USDA’s National Milk Testing Strategy (NMTS) in Nevada.  USDA APHIS continues to work with the Nevada Department of Agriculture by conducting additional on-farm investigation, testing, and gathering additional epidemiological information to better understand this detection and limit further disease spread.  This is the first detection of this virus genotype in dairy cattle (all previous detections in dairy cattle have been HPAI H5N1 clade 2.3.4.4b, genotype B3.13 ).  Genotype D1.1 represents the predominant genotype in the North American flyways this past fall an...
Post a Comment
Read more »

#USA, After #Birdflu Detected in Local #Cat, County #Health Officials Say #Pet Owners Should Contact Veterinarian When Their Pets are Sick

By G.M.
Redwood City — State veterinary and health officials have confirmed a case of H5N1 (bird flu) in a domestic stray cat in San Mateo County.  The infection, which is not related to the recent instance of bird flu in a backyard flock , was found in a stray cat in Half Moon Bay that had been taken in by a family .  When it showed symptoms, they took it to Peninsula Humane Society, whose veterinarians examined it and requested testing. Lab results confirmed H5N1.  It is not known how the cat was infected and it was euthanized due to its condition. Cats may be exposed to bird flu by consuming infected bird , being in environments contaminated with the virus and consuming unpasteurized milk from infected cows or raw food. Inside domestic animals, such as cats and dogs, that go outside are also at risk of infection.​​​​​​​ According to the Centers for Disease Control and Prevention, the risk of cats spreading H5N1 to people is extremely low, though it is possible for cats to spre...
Post a Comment
Read more »

#USA, Novel #Influenza A #H5N1 Virus: One Pediatric Case in #California {FluView}

By G.M.
 {Excerpt} One confirmed human infection with influenza A(H5) virus was reported to CDC this week. To date, human-to-human transmission of influenza A(H5) virus has not been identified in the United States. This case was reported by the California Department of Public Health and occurred in a child less than 18 years old with no known contact with influenza A(H5N1) virus-infected animals or humans . The investigation into the source of infection for this case is ongoing , and no human-to-human transmission has been identified. A specimen from the individual was tested at a public health laboratory using the CDC influenza A(H5) assay before being sent to CDC for further testing. The specimen was positive for influenza A(H5) virus using diagnostic RT-PCR at CDC. Additional analysis including genetic sequencing is underway. In response to this detection, additional case investigation and contact monitoring are being conducted by public health officials in California. There have now be...
Post a Comment
Read more »