Abstract Many pathogens, both those with human spillover potential as well as avian-specific viruses, are maintained in wild bird populations . While much surveillance for influenza A viruses (IAVs) is performed annually, surveillance for other pathogens is limited. Sampling of wild birds is often time-consuming , labour-intensive, involves physically handling wild birds, often limited in sample size, and involves handling of potentially infected birds , posing an increased risk of direct exposure for personnel. Given this, additional methods for surveillance are needed. Longitudinal, bi-weekly fecal and sediment sampling was performed at various sites in southern Manitoba, Canada , particularly focused in Winnipeg from May - October 2025. Sites were chosen based on the suitability of the area for waterfowl habitat , the presence of waterfowl in the area, as well as sites in proximity to reported outbreaks of H5N1 influenza virus . Fecal and sediment samples were collected and sc...