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Showing posts from December 27, 2025

A #Market-Based #Sentinel #Surveillance for an Early #Detection of Viral #Outbreaks

  Abstract Mexico has experienced recurrent viral epidemics of substantial intensity, including hyperendemic dengue, COVID-19, and recent reports of avian influenza A (H5N1) infections in birds , which pose an ongoing risk of zoonotic transmission . Mexico was also the location for the earliest detection of the pdmH1N1 virus during the 2009 influenza A pandemic. Under a One Health framework , markets represent a unique opportunity for low-cost virus monitoring at the human-animal interface . Under the hypothesis that these represent sentinel sites for an early virus detection , we implemented a pilot surveillance program at the central market of Merida city, Yucatan , Mexico, considered a regional hotspot for multiple and recent viral outbreaks . Longitudinal sampling was carried out over 11 months at 1-to-6-week intervals from April 2022 to February 2023 . We used multi-type surveillance in mosquitoes, live poultry, and wastewater . All samples were screened using RT-qPCR . Positi...

History of Mass Transportation: The Spoornet Class 14E Electric Locomotive

Von Col AndrĂ© Kritzinger, CC BY-SA 3.0,  https://commons.wikimedia.org/w/index.php?curid=11506789 Source:  Link:  https://de.wikipedia.org/wiki/Spoornet-Klasse_14E ____

History of Mass Transportation: The EC 250 ''Giruno'' Electric Multiple Unit Train

Par Daniel Wipf — Travail personnel, CC BY-SA 4.0,  https://commons.wikimedia.org/w/index.php?curid=59022313 Source:  Link:  https://fr.wikipedia.org/wiki/EC_250_%C2%AB_Giruno_%C2%BB ____

A #VSV #vector #vaccine simultaneously targeting #H5N1 HA & M2 induces robust neutralizing and ADCC #antibody responses & provides full protection vs lethal #H5N1 infection in mouse model

  Abstract Human (avian) influenza A viruses, especially highly pathogenic avian influenza (HPAI) viruses, pose a significant public health threat , and a multivalent vaccine is the primary prophylactic measure to control these viruses. To establish such a vaccine, we generated two multivalent vesicular stomatitis virus (VSV)-based vaccine candidates (V-EtM2e/H505 and V-EtM2e/H522) and characterized their ability to induce protective immune responses. Our results revealed that vaccine immunization in mice induced high humoral immune responses against both the HPAI hemagglutinin (HA) protein and the ectodomain of M2 (M2e) protein . Intriguingly, vaccine-immunized mouse sera exhibited highly efficient neutralizing activity against the corresponding H5 pseudovirus and mediated potent and broad antibody-dependent cellular cytotoxicity (ADCC) activity against M2e derived from human and avian influenza H5, H1, H3, and H7 viruses . Furthermore, both intranasal and intramuscular immunizati...