#Remdesivir as a potent #antiviral against prototype and current #epidemic #Oropouche virus #strains (BeAn19991 and PE-IAM4637)
Highlights • We generated a recombinant reporter OROV that expresses the eGFP fluorescent protein in infected cells. • We found that remdesivir efficiently inhibited the replication of Oropouche virus (OROV) using this reporter OROV. • We demonstrated strain-dependent differences in the replication efficiency of OROV. Abstract The Oropouche virus (OROV), an orthobunyavirus transmitted by biting midges, is the causative agent of Oropouche fever , which has caused multiple outbreaks in South and Central America . During the most recent epidemic in 2023–2025, more than 25,000 laboratory-confirmed cases were reported in Brazil , and no licensed antivirals have been reported to be effective date. In this study, we generated a recombinant OROV-expressing enhanced green fluorescent protein (rOROV/GFP) to facilitate rapid and sensitive antiviral evaluation . Growth kinetics demonstrated that rOROV/GFP replicated less efficiently than wild-type rOROV and that the histori...