Skip to main content

Metabolomic Profiling of #Plasma Reveals Differential #Disease Severity #Markers in avian #influenza A(#H7N9) infection Patients

Image
By G.M.

Highlights

• The characteristics of plasma metabolome in H7N9 patients were first revealed.

• It was discovered that lipid-like molecules were downregulated in death group.

• Metabolites of the tryptophan metabolic pathway were elevated in death group.

• The metabolite-based machine learning achieved an AUC of 0.929 on the test set.


Abstract

Objectives

Avian influenza such as H7N9 is currently a major global public health risk, and at present, there is a lack of relevant diagnostic and treatment markers.

Methods

We collected plasma samples from 104 confirmed H7N9 patients, 31 of whom died. Plasma metabolites were detected by UHPLC-HRMS, and a survival prediction model based on metabolites was constructed by machine learning models.

Results

A total of 1536 metabolites were identified in the plasma samples of H7N9 patients, of which 64 metabolites were up-regulated and 35 metabolites were down-regulated in the death group. The enrichment analysis of Tryptophan metabolism, Porphyrin metabolism and Riboflavin metabolism were significantly up-regulated in the death group. We found that most Lipids and lipid−like molecules were down-regulated in the death group, and Organoheterocyclic compounds were significantly up-regulated in the death group. A machine learning model was constructed for predicting mortality based on Porphobilinogen, 5-Hydroxyindole-3-acetic acid, L-Kynurenine, Biliverdin, and D-Dimer. The AUC on the test set was 0.929.

Conclusions

We first revealed the plasma metabolomic characteristics of H7N9 patients and found that a machine learning model based on plasma metabolites could predict the risk of death for H7N9 in the early stage of admission.

Source: International Journal of Infectious Diseases, https://www.ijidonline.com/article/S1201-9712(25)00181-X/fulltext

____

Labels:

Comments

Post a Comment

Popular posts from this blog

#Neuroinvasive #Oropouche virus in a patient with #HIV from extra-Amazonian #Brazil

By G.M.
{Excerpt} A novel reassortant Oropouche virus (OROV) lineage (with medium [M], large [L], and small [S] RNA segments : M1L2S2) has driven Brazil's largest and most geographically widespread OROV epidemic , expanding beyond the endemic Amazon basin to establish local transmission across multiple Brazilian states and other previously unaffected Latin American countries . The rapid spread of this lineage underscores its evolutionary potential and reinforces its significance as a public health threat .1 Similar to chikungunya and Zika viruses, expanding arboviruses can exhibit unexpected clinical and epidemiological shifts , including vertical transmissions , neuroinvasive effects, and potentially fatal outcomes.2–4 Although OROV typically causes self-limited febrile illness, accumulating clinical and experimental evidence suggests neurotropic potential .5 This Correspondence describes the first confirmed case of neuroinvasive OROV infection caused by the emergent M1L2S2 lineage in ext...
Post a Comment
Read more »

Stability of #influenza viruses in the #milk of #cows and #sheep

By G.M.
Abstract In late 2023, H5N1 high pathogenicity avian influenza (HPAIV) started circulating in dairy cattle in the USA . High viral titres were detected in milk from infected cows , raising concerns about onwards human infections . Although pasteurisation was shown to effectively inactivate influenza viruses in milk, unpasteurised milk still poses a risk of infection, both from occupational exposure in dairies and from the consumption of raw milk. We therefore assessed how long influenza viruses could remain infectious for in milk without heat inactivation. We examined the stability of a panel of influenza viruses in milk , including a contemporary H5N1 HPAIV and a variety of other influenza A and D viruses. We incubated viruses in cows' milk under laboratory conditions : at room temperature to simulate exposure in dairies and at 4°C to simulate exposure to refrigerated raw milk. Following an isolated report of H5N1 viral RNA being detected in milk from a sheep in the UK , we also c...
Post a Comment
Read more »

#Evidence of #Viremia in Dairy #Cows Naturally Infected with #Influenza A {#H5N1} Virus, #California, #USA

By G.M.
Abstract We confirmed influenza A virus (IAV) by PCR in serum from 18 cows on 3 affected dairy farms in California, USA . Our findings indicate the presence of viremia and might help explain IAV transmission dynamics and shedding patterns in cows. An understanding of those dynamics could enable development of IAV mitigation strategies. Source: US Centers for Disease Control and Prevention,  https://wwwnc.cdc.gov/eid/article/31/7/25-0134_article ____
Post a Comment
Read more »