Characterization of a reassortant #H3N2 swine #influenza virus with 2009 pandemic internal #genes and enhanced potential for zoonotic #risk
Highlights • A swine influenza virus H3N2 subtype was isolated during epidemiological survey. • It is a complex and novel reassortant , and acquired accumulation of adaptive mutations. • Both rescue and parent strains demonstrated efficient replication in mammalian cells. • Key residues of the H3N2 HA collectively enhance the binding preference for human-type receptor. • The rescued H3N2 cause significant pulmonary pathological damage in mice. Abstract Pigs serve as key "mixing vessels" for influenza A viruses, playing a critical role in cross-species transmission , while the H3N2 subtype represents an important lineage within the swine influenza virus (SIV) family. In this study, a novel reassortant H3N2 SIV strain , designated A/Swine/Jiangsu/YZ07/2024 , was isolated from pigs exhibiting clinical symptoms in Northern Jiangsu , China during epidemiological survey . Genetic analysis revealed that the virus is a complex reassortant, with the internal ...