Abstract Influenza D virus (IDV), primarily found in livestock species, has demonstrated cross-species transmission potential , yet its threat to humans remains poorly understood . Here, we curated a panel of IDV isolates collected during field surveillance from 2011 to 2020 from swine and cattle to assess their ability to infect human airway cells as a proxy for zoonotic threat assessment. Using lung epithelial cell lines , primary well-differentiated airway epithelial cultures, and precision-cut lung slices , we demonstrated that IDV efficiently propagates in cells and tissues from the human respiratory tract , reaching titers comparable to human influenza A virus (IAV). Infection kinetics in primary porcine airway cultures and respiratory tissues mirrored those from human , suggesting similar infectivity across species. To define host responses to IDV infection, we evaluated innate immune sensing and downstream interferon signaling in human respiratory cells. IDV infection res...
Media Monitoring for Signals about Emerging Threats