ETIDIOH - NEW

{Excerpts} Avian Influenza Report - VOLUME 22, NUMBER 11 - Reporting period: March 8, 2026 – March 14, 2026 (Week 11) (Published on March 17, 2026)   (...) -- Date of report :  14/03/2026 -- Country :  Cambodia -- Province / Region District / City :  Banteay Meanchey province, Preah Netr, Preah district -- Sex :  Female -- Age :  45 -- Condition at time of reporting :  Hospitalised -- Subtype of virus : H5N1   (...) Source:  Link:  https://www.chp.gov.hk/files/pdf/2026_avian_influenza_report_vol22_wk11.pdf ____

  Abstract Background   Tropical low – and middle –income countries are highly vulnerable to zoonoses and vector-borne diseases , with risks amplified by climatic events , environmental change , and limited surveillance capacity. Cambodia is particularly exposed due to its ecological diversity, seasonal flooding , and rapidly changing land use . Globally, however, field based One Health approaches remain under –implemented, limiting practical evidence on how to address these complex threats.  Methods   This protocol describes a longitudinal One Health study conducted in three villages of Battambang province, Cambodia , designed to investigate the prevalence and transmission dynamics of zoonotic and potentially zoonotic pathogens at the human –animal –environment interface . The study examines how vector density, diversity, and pathogen circulation are influenced by hydrological variation and seasonality , and assesses the sociodemographic, behavioral, and environment...

  Abstract Influenza A viruses (IAV) remain a persistent One Health threat, and whole-genome sequencing from wastewater offers a promising surveillance tool . However, IAV is at low abundance in wastewater , making it difficult to sequence . We benchmarked four targeted enrichment methods suited for whole-genome sequencing including custom and off-the-shelf amplicon and probe-based methods . Our custom HA tiled-amplicon panel was sensitive, fast, and cost-effective, making it suitable for monitoring low-abundance seasonal variants of known subtypes . However, its reliance on conserved and intact primer-binding sites limited primer design to fewer subtypes. A previously published universal amplicon method targeted all IAV subtypes, but it performed poorly in wastewater due to its reliance on intact genome segments. Probe-capture methods were resilient to RNA degradation and mismatches, potentially enabling broader surveillance and detection of emerging strains. However, probes were ...